PMID- 27460927
OWN - NLM
STAT- MEDLINE
DCOM- 20171106
LR  - 20191210
IS  - 1756-6606 (Electronic)
IS  - 1756-6606 (Linking)
VI  - 9
IP  - 1
DP  - 2016 Jul 26
TI  - Hippocampus-based contextual memory alters the morphological characteristics of 
      astrocytes in the dentate gyrus.
PG  - 72
LID - 10.1186/s13041-016-0253-z [doi]
LID - 72
AB  - Astrocytes have been reported to exist in two states, the resting and the 
      reactive states. Morphological changes in the reactive state of astrocytes 
      include an increase in thickness and number of processes, and an increase in the 
      size of the cell body. Molecular changes also occur, such as an increase in the 
      expression of glial fibrillary acidic protein (GFAP). However, the morphological 
      and molecular changes during the process of learning and memory have not been 
      elucidated. In the current study, we subjected Fvb/n mice to contextual fear 
      conditioning, and checked for morphological and molecular changes in astrocytes. 
      1 h after fear conditioning, type II and type III astrocytes exhibited a unique 
      status with an increased number of processes and decreased GFAP expression which 
      differed from the typical resting or reactive state. In addition, the protein 
      level of excitatory excitatory amino acid transporter 2 (EAAT2) was increased 1 h 
      to 24 h after contextual fear conditioning while EAAT1 did not show any 
      alterations. Connexin 43 (Cx43) protein was found to be increased at 24 h after 
      fear conditioning. These data suggest that hippocampus-based contextual memory 
      process induces changes in the status of astrocytes towards a novel status 
      different from typical resting or reactive states. These morphological and 
      molecular changes may be in line with functional changes.
FAU - Choi, Moonseok
AU  - Choi M
AD  - Department of Pharmacology and Biomedical Sciences, College of Medicine, Seoul 
      National University, 103 Daehakro, Jongro-gu, 110-799, Seoul, Republic of Korea.
FAU - Ahn, Sangzin
AU  - Ahn S
AD  - Department of Pharmacology and Biomedical Sciences, College of Medicine, Seoul 
      National University, 103 Daehakro, Jongro-gu, 110-799, Seoul, Republic of Korea.
FAU - Yang, Eun-Jeong
AU  - Yang EJ
AD  - Department of Pharmacology and Biomedical Sciences, College of Medicine, Seoul 
      National University, 103 Daehakro, Jongro-gu, 110-799, Seoul, Republic of Korea.
FAU - Kim, Hyunju
AU  - Kim H
AD  - Department of Pharmacology and Biomedical Sciences, College of Medicine, Seoul 
      National University, 103 Daehakro, Jongro-gu, 110-799, Seoul, Republic of Korea.
FAU - Chong, Young Hae
AU  - Chong YH
AD  - Department of Microbiology, School of Medicine, Ewha Womans University, 911-1, 
      Mok-6-dong, Yangcheonku, Seoul, 158-710, Republic of Korea.
FAU - Kim, Hye-Sun
AU  - Kim HS
AD  - Department of Pharmacology and Biomedical Sciences, College of Medicine, Seoul 
      National University, 103 Daehakro, Jongro-gu, 110-799, Seoul, Republic of Korea. 
      hyisun@snu.ac.kr.
AD  - Seoul National University College of Medicine, Seoul National University Bundang 
      Hospital, Sungnam, 463-707, Republic of Korea. hyisun@snu.ac.kr.
AD  - Seoul National University College of Medicine, Bundang Hospital, Sungnam, 
      Bundang-Gu, Republic of Korea. hyisun@snu.ac.kr.
AD  - Neuroscience Research Institute, College of Medicine, Seoul National University, 
      103 Daehakro, Jongro-gu, Seoul, Republic of Korea. hyisun@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160726
PL  - England
TA  - Mol Brain
JT  - Molecular brain
JID - 101468876
RN  - 0 (Connexin 43)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg4 protein, mouse)
RN  - 0 (Excitatory Amino Acid Transporter 2)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Membrane Proteins)
RN  - 1K7B1OED4N (2-Aminoadipic Acid)
RN  - EC 2.7.4.8 (Guanylate Kinases)
SB  - IM
MH  - 2-Aminoadipic Acid/administration & dosage
MH  - Animals
MH  - Astrocytes/*pathology
MH  - *Cell Shape
MH  - Conditioning, Psychological
MH  - Connexin 43/metabolism
MH  - Dentate Gyrus/*pathology
MH  - Disks Large Homolog 4 Protein
MH  - Excitatory Amino Acid Transporter 2/metabolism
MH  - Fear
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Guanylate Kinases/metabolism
MH  - Hippocampus/*physiology
MH  - Membrane Proteins/metabolism
MH  - Memory/*physiology
MH  - Mice
PMC - PMC4962445
OTO - NOTNLM
OT  - Astrocyte
OT  - Connexin 43
OT  - EAATs
OT  - GFAP
OT  - Hippocampus-based contextual memory
OT  - Morphological changes
EDAT- 2016/07/28 06:00
MHDA- 2017/11/07 06:00
PMCR- 2016/07/26
CRDT- 2016/07/28 06:00
PHST- 2016/01/20 00:00 [received]
PHST- 2016/07/22 00:00 [accepted]
PHST- 2016/07/28 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/11/07 06:00 [medline]
PHST- 2016/07/26 00:00 [pmc-release]
AID - 10.1186/s13041-016-0253-z [pii]
AID - 253 [pii]
AID - 10.1186/s13041-016-0253-z [doi]
PST - epublish
SO  - Mol Brain. 2016 Jul 26;9(1):72. doi: 10.1186/s13041-016-0253-z.