PMID- 27461828
OWN - NLM
STAT- MEDLINE
DCOM- 20170323
LR  - 20190125
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 212
IP  - 8
DP  - 2016 Aug
TI  - Upregulation of long noncoding RNA ZFAS1 predicts poor prognosis and prompts 
      invasion and metastasis in colorectal cancer.
PG  - 690-5
LID - S0344-0338(16)30085-1 [pii]
LID - 10.1016/j.prp.2016.05.003 [doi]
AB  - PURPOSE: ZFAS1 is a newly identified long non-coding RNA. The present study 
      investigated the relationships of ZFAS1 expression with clinicalpathologic 
      parameters and the prognosis of patients with colorectal cancer (CRC). The 
      effects of ZFAS1 on cell migration, invasion and metastasis were also been 
      evaluated in CRC cell lines. METHODS: A total of 159 paired primary CRC tissues 
      and adjacent noncancerous tissues (NCTs) were collected and were tested for the 
      expression of ZFAS1. Cell migration and invasion were measured by wound-healing 
      and Transwell assays, respectively. Tumor metastasis was assessed using a lung 
      metastasis model of nude mouse. RESULTS: The ZFAS1 expression was significantly 
      upregulated in 47.8% (76 of 159) of cancerous tissues compared with 
      NCTs(p<0.0001), and was higher in metastatic tumors than in corresponding primary 
      CRC tumors (p=0.010). ZFAS1 expression in CRC was positively correlated with 
      lymphatic invasion (p=0.045) and TNM stage (p=0.003). Patients with increased 
      ZFAS1 expression showed shorter relapse-free survival and overall survival 
      (p<0.05). Cox multivariate analyses confirmed that ZFAS1 expression is an 
      independent prognostic factor in CRC (p<0.05). The knockdown of ZFAS1 decreased 
      cell migration and invasive ability of LoVo and HCT116 cells in vitro (p<0.05). 
      Moreover, silencing ZFAS1 expression inhibited the in vivo metastasis of CRC 
      cells (p<0.05). CONCLUSIONS: The expression of ZFAS1 is positively correlated 
      with TNM stage and appears to be a new prognostic factor in CRC. ZFAS1 promptes 
      cell migration, invasive and metastasis ability of CRC cells.
CI  - Copyright (c) 2016 Elsevier GmbH. All rights reserved.
FAU - Wang, Weili
AU  - Wang W
AD  - Department of Surgery, The Second Affiliated Hospital of Soochow University, 
      China.
FAU - Xing, Chungen
AU  - Xing C
AD  - Department of Surgery, The Second Affiliated Hospital of Soochow University, 
      China. Electronic address: xingcg@126.com.
LA  - eng
PT  - Journal Article
DEP - 20160512
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (ZFAS1 long non-coding RNA, human)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - Colorectal Neoplasms/*genetics/metabolism/mortality/pathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Mice
MH  - Mice, Nude
MH  - Middle Aged
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - Neoplasm Staging
MH  - Prognosis
MH  - RNA Interference
MH  - RNA, Long Noncoding/*genetics/metabolism
MH  - RNA, Small Interfering
MH  - Survival Rate
MH  - *Up-Regulation
OTO - NOTNLM
OT  - Colorectal cancer
OT  - Metastasis
OT  - Prognosis
OT  - ZFAS1
OT  - lncRNA
EDAT- 2016/07/28 06:00
MHDA- 2017/03/24 06:00
CRDT- 2016/07/28 06:00
PHST- 2016/01/11 00:00 [received]
PHST- 2016/04/26 00:00 [revised]
PHST- 2016/05/03 00:00 [accepted]
PHST- 2016/07/28 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2017/03/24 06:00 [medline]
AID - S0344-0338(16)30085-1 [pii]
AID - 10.1016/j.prp.2016.05.003 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2016 Aug;212(8):690-5. doi: 10.1016/j.prp.2016.05.003. Epub 
      2016 May 12.