PMID- 27462273
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160727
LR  - 20200930
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 7
DP  - 2016
TI  - Increased Hepatic Fatty Acids Uptake and Oxidation by LRPPRC-Driven Oxidative 
      Phosphorylation Reduces Blood Lipid Levels.
PG  - 270
LID - 10.3389/fphys.2016.00270 [doi]
LID - 270
AB  - Hyperlipidemia is one of the major risk factors of atherosclerosis and other 
      cardiovascular diseases. This study aimed to investigate the impact of leucine 
      rich pentatricopeptide repeat containing protein (LRPPRC)-driven hepatic 
      oxidative phoshorylation on blood lipid levels. The hepatic LRPPRC level was 
      modulated by liver-specific transgenic or adeno-associated virus 8 carried shRNA 
      targeting Lrpprc (aav-shLrpprc). Mice were fed with a high fat diet to induce 
      obesity. Gene expression was analyzed by quantitative real-time PCR and / or 
      western blot. The hepatic ATP level, hepatic and serum lipids contents, and 
      mitochondria oxidative phosphorylation (OxPhos) complex activities were measured 
      using specific assay kits. The uptake and oxidation of fatty acid by hepatocytes 
      were assessed using (14)C-palmitate. LRPPRC regulated the expression of genes 
      encoded by mitochondrial genome but not those by nuclear genome involved in 
      mitochondria biogenesis, OxPhos, and lipid metabolism. Increased OxPhos in liver 
      mediated by LRPPRC resulted in the increase of hepatic ATP level. Lrpprc promoted 
      palmitate uptake and oxidation by hypatocytes. The hepatic and serum triglyceride 
      and total cholesterol levels were inversely associated with the hepatic LRPPRC 
      level. These data demonstrated that LRPPRC-driven hepatic OxPhos could promote 
      fatty acids uptake and oxidation by hepatocytes and reduce both hepatic and 
      circulating triglyceride and cholesterol levels.
FAU - Lei, Shi
AU  - Lei S
AD  - College of Biological and Pharmaceutical Sciences, China Three Gorges University 
      Yichang, China.
FAU - Sun, Run-Zhu
AU  - Sun RZ
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University 
      Xi'an, China.
FAU - Wang, Di
AU  - Wang D
AD  - Department of Laboratory Medicine, Huashan Hospital North, Shanghai Medical 
      School, Fudan University Shanghai, China.
FAU - Gong, Mei-Zhen
AU  - Gong MZ
AD  - College of Biological and Pharmaceutical Sciences, China Three Gorges University 
      Yichang, China.
FAU - Su, Xiang-Ping
AU  - Su XP
AD  - College of Biological and Pharmaceutical Sciences, China Three Gorges University 
      Yichang, China.
FAU - Yi, Fei
AU  - Yi F
AD  - College of Biological and Pharmaceutical Sciences, China Three Gorges University 
      Yichang, China.
FAU - Peng, Zheng-Wu
AU  - Peng ZW
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University 
      Xi'an, China.
LA  - eng
PT  - Journal Article
DEP - 20160712
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC4941416
OTO - NOTNLM
OT  - LRPPRC
OT  - fatty acids
OT  - mitochondria
OT  - oxidative phosphorylation
OT  - triglyceride
EDAT- 2016/07/28 06:00
MHDA- 2016/07/28 06:01
PMCR- 2016/07/12
CRDT- 2016/07/28 06:00
PHST- 2016/02/27 00:00 [received]
PHST- 2016/06/16 00:00 [accepted]
PHST- 2016/07/28 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2016/07/28 06:01 [medline]
PHST- 2016/07/12 00:00 [pmc-release]
AID - 10.3389/fphys.2016.00270 [doi]
PST - epublish
SO  - Front Physiol. 2016 Jul 12;7:270. doi: 10.3389/fphys.2016.00270. eCollection 
      2016.