PMID- 27462365
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160727
LR  - 20200930
IS  - 1750-9378 (Print)
IS  - 1750-9378 (Electronic)
IS  - 1750-9378 (Linking)
VI  - 11
DP  - 2016
TI  - Human herpesvirus multiplex ddPCR detection in brain tissue from low- and 
      high-grade astrocytoma cases and controls.
PG  - 32
LID - 10.1186/s13027-016-0081-x [doi]
LID - 32
AB  - BACKGROUND: Glioblastoma (GBM) is a fatal CNS malignancy, representing 50 % of 
      all gliomas with approximately 12-18 months survival time after initial 
      diagnosis. Recently, the human herpesvirus cytomegalovirus (CMV) has been 
      suggested to have an oncogenic role, yet this association remains controversial. 
      In addition, human herpesvirus 6 (HHV-6) and Epstein-Barr virus (EBV) have also 
      been associated with low-grade gliomas, but few studies have examined HHV-6 and 
      EBV in glioblastomas. Droplet digital PCR (ddPCR) is a highly precise diagnostic 
      tool that enables the absolute quantification of target DNA. This study examines 
      the association between multiple human herpesviruses and astrocytomas. METHODS: 
      This study analyzed 112 brain tissue specimens, including 45 glioblastoma, 12 
      astrocytoma grade III, 2 astrocytoma grade II, 4 astrocytoma grade I, and 49 
      controls. All brain tissue samples were de-identified and pathologically 
      confirmed. Each tissue block was sectioned for DNA extraction and CMV, EBV, 
      HHV-6A and HHV-6B, and a cellular housekeeping gene were amplified by ddPCR. 
      RESULTS: Neither CMV nor HHV-6A were detected in any of the astrocytoma samples. 
      However, HHV-6B (p = 0.147) and EBV (p = 0.049) had a higher positivity frequency 
      in the GBM compared to the controls. CONCLUSION: The undetectable CMV DNA in the 
      astrocytoma cohort does not support the observation of an increased prevalence of 
      CMV DNA in GBM, as reported in other studies. EBV has a significantly higher 
      positivity in the GBM cohort compared to the controls, while HHV-6B has a higher 
      but not statistically significant positivity in the case cohort. Whether these 
      viruses play an oncogenic role in GBM remains to be further investigated.
FAU - Lin, Cheng-Te Major
AU  - Lin CT
AD  - The National Institute of Neurological Disorders and Stroke, National Institutes 
      of Health, BG 10 RM 5C103 10 Center Dr., Bethesda, MD 20892 USA ; School of 
      Medicine and Health Sciences, The George Washington University, Ross Hall 2300 
      Eye Street, NW, Washington, DC 20037 USA.
FAU - Leibovitch, Emily C
AU  - Leibovitch EC
AD  - The National Institute of Neurological Disorders and Stroke, National Institutes 
      of Health, BG 10 RM 5C103 10 Center Dr., Bethesda, MD 20892 USA ; School of 
      Medicine and Health Sciences, The George Washington University, Ross Hall 2300 
      Eye Street, NW, Washington, DC 20037 USA.
FAU - Almira-Suarez, M Isabel
AU  - Almira-Suarez MI
AD  - School of Medicine and Health Sciences, The George Washington University, Ross 
      Hall 2300 Eye Street, NW, Washington, DC 20037 USA.
FAU - Jacobson, Steven
AU  - Jacobson S
AD  - The National Institute of Neurological Disorders and Stroke, National Institutes 
      of Health, BG 10 RM 5C103 10 Center Dr., Bethesda, MD 20892 USA.
LA  - eng
PT  - Journal Article
DEP - 20160726
PL  - England
TA  - Infect Agent Cancer
JT  - Infectious agents and cancer
JID - 101276559
PMC - PMC4960850
OTO - NOTNLM
OT  - Astrocytoma
OT  - CMV
OT  - EBV
OT  - Glioblastoma
OT  - HHV-6A
OT  - HHV-6B
OT  - Herpesvirus
OT  - ddPCR
EDAT- 2016/07/28 06:00
MHDA- 2016/07/28 06:01
PMCR- 2016/07/26
CRDT- 2016/07/28 06:00
PHST- 2016/03/31 00:00 [received]
PHST- 2016/05/25 00:00 [accepted]
PHST- 2016/07/28 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2016/07/28 06:01 [medline]
PHST- 2016/07/26 00:00 [pmc-release]
AID - 81 [pii]
AID - 10.1186/s13027-016-0081-x [doi]
PST - epublish
SO  - Infect Agent Cancer. 2016 Jul 26;11:32. doi: 10.1186/s13027-016-0081-x. 
      eCollection 2016.