PMID- 27462461
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160727
LR  - 20200930
IS  - 2056-5968 (Print)
IS  - 2056-5968 (Electronic)
IS  - 2056-5968 (Linking)
VI  - 2
DP  - 2016
TI  - A genome-scale CRISPR-Cas9 screening method for protein stability reveals novel 
      regulators of Cdc25A.
PG  - 16014
LID - 10.1038/celldisc.2016.14 [doi]
AB  - The regulation of stability is particularly crucial for unstable proteins in 
      cells. However, a convenient and unbiased method of identifying regulators of 
      protein stability remains to be developed. Recently, a genome-scale CRISPR-Cas9 
      library has been established as a genetic tool to mediate loss-of-function 
      screening. Here, we developed a protein stability regulators screening assay 
      (Pro-SRSA) by combining the whole-genome CRISPR-Cas9 library with a 
      dual-fluorescence-based protein stability reporter and high-throughput sequencing 
      to screen for regulators of protein stability. Using Cdc25A as an example, 
      Cul4B-DDB1(DCAF8) was identified as a new E3 ligase for Cdc25A. Moreover, the 
      acetylation of Cdc25A at lysine 150, which was acetylated by p300/CBP and 
      deacetylated by HDAC3, prevented the ubiquitin-mediated degradation of Cdc25A by 
      the proteasome. This is the first study to report that acetylation, as a novel 
      posttranslational modification, modulates Cdc25A stability, and we suggest that 
      this unbiased CRISPR-Cas9 screening method at the genome scale may be widely used 
      to globally identify regulators of protein stability.
FAU - Wu, Yuanzhong
AU  - Wu Y
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Zhou, Liwen
AU  - Zhou L
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Wang, Xin
AU  - Wang X
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Lu, Jinping
AU  - Lu J
AD  - Clinical Laboratory and Medical Research Center, Zhuhai Hospital, Jinan 
      University, Zhuhai People's Hospital , Zhuhai, China.
FAU - Zhang, Ruhua
AU  - Zhang R
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Liang, Xiaoting
AU  - Liang X
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Wang, Li
AU  - Wang L
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Deng, Wuguo
AU  - Deng W
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Zeng, Yi-Xin
AU  - Zeng YX
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine , Guangzhou, China.
FAU - Huang, Haojie
AU  - Huang H
AD  - Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine 
      , Rochester, MN, USA.
FAU - Kang, Tiebang
AU  - Kang T
AD  - Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South 
      China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, China; 
      Guangdong Provincial Key Laboratory of Tumor Targeted Drugs, Guangzhou Doublle 
      Bioproducts Co. Ltd., Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20160524
PL  - England
TA  - Cell Discov
JT  - Cell discovery
JID - 101661034
CIN - Chin J Cancer. 2016 Jun 29;35(1):60. PMID: 27357860
PMC - PMC4877570
OTO - NOTNLM
OT  - CRISPR-Cas9 screening
OT  - Cdc25A
OT  - acetylation
OT  - protein stability
OT  - ubiquitination
EDAT- 2016/07/28 06:00
MHDA- 2016/07/28 06:01
PMCR- 2016/05/24
CRDT- 2016/07/28 06:00
PHST- 2016/03/11 00:00 [received]
PHST- 2016/03/17 00:00 [accepted]
PHST- 2016/07/28 06:00 [entrez]
PHST- 2016/07/28 06:00 [pubmed]
PHST- 2016/07/28 06:01 [medline]
PHST- 2016/05/24 00:00 [pmc-release]
AID - celldisc201614 [pii]
AID - 10.1038/celldisc.2016.14 [doi]
PST - epublish
SO  - Cell Discov. 2016 May 24;2:16014. doi: 10.1038/celldisc.2016.14. eCollection 
      2016.