PMID- 27464625
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20170206
IS  - 1881-7823 (Electronic)
IS  - 1881-7815 (Linking)
VI  - 10
IP  - 4
DP  - 2016 Sep 5
TI  - The protective effects of human umbilical cord mesenchymal stem cells on damaged 
      ovarian function: A comparative study.
PG  - 265-76
LID - 10.5582/bst.2016.01125 [doi]
AB  - Numerous studies have reported that human umbilical cord mesenchymal stem cell 
      (hUCMSC) therapy can rescue the structure and function of injured tissues. The 
      aims of this study were to explore the protective role of hUCMSC transplantation 
      in a model of accelerated ovarian aging and to compare 2 methods of transplanting 
      hUCMSCs, i.e. i) via intravenous injection (IV) and ii) in situ ovarian micro 
      injection (MI). Female mice were subjected to superovulation and ozone inhalation 
      to create a model of accelerated ovarian aging with a decline in both the 
      quantity and quality of oocytes. Cells were transplanted via IV or MI, and 
      ovaries were removed after 2 weeks or 1 month of treatment. Ovarian reserve and 
      function were evaluated based on the follicle counts, hormone levels, the estrous 
      cycle, and reproductive performance. Cell tracking, terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL), real-time polymerase chain 
      reaction (PCR), and Western blot analysis were used to assess the inner 
      mechanisms of injury and repair. Results indicated that ovarian function 
      increased significantly after treatment with hUCMSCs. Immunofluorescence revealed 
      reduced TUNEL staining and a decreased percentage of apoptotic cells. A higher 
      level of expression of anti-apoptotic and antioxidant enzymes was noted in the 
      ovaries of groups treated with hUCMSCs. These parameters were enhanced more when 
      mice were treated with hUCMSCs for 1 month than when they treated with hUCMSCs 
      for 2 weeks. IV was better able to restore ovarian function than MI. These 
      results suggest that both methods of transplantation may improve ovarian function 
      and that IV transplantation of hUCMSCs can significantly improve ovarian function 
      and structural parameters more than MI transplantation of hUCMSCs can.
FAU - Zhang, Jinjin
AU  - Zhang J
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Huazhong University of 
      Science and Technology.
FAU - Xiong, Jiaqiang
AU  - Xiong J
FAU - Fang, Li
AU  - Fang L
FAU - Lu, Zhiyong
AU  - Lu Z
FAU - Wu, Meng
AU  - Wu M
FAU - Shi, Liangyan
AU  - Shi L
FAU - Qin, Xian
AU  - Qin X
FAU - Luo, Aiyue
AU  - Luo A
FAU - Wang, Shixuan
AU  - Wang S
LA  - eng
PT  - Journal Article
DEP - 20160726
PL  - Japan
TA  - Biosci Trends
JT  - Bioscience trends
JID - 101502754
RN  - 0 (Antioxidants)
SB  - IM
EIN - Biosci Trends. 2018;12(4):E1. PMID: 30232317
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Apoptosis/genetics
MH  - Cell Tracking
MH  - *Cell- and Tissue-Based Therapy
MH  - Estrous Cycle
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oocytes/pathology
MH  - Ovarian Diseases/*therapy
MH  - Ovarian Reserve
MH  - Real-Time Polymerase Chain Reaction
MH  - Umbilical Cord/*cytology
EDAT- 2016/07/29 06:00
MHDA- 2017/02/07 06:00
CRDT- 2016/07/29 06:00
PHST- 2016/07/29 06:00 [entrez]
PHST- 2016/07/29 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
AID - 10.5582/bst.2016.01125 [doi]
PST - ppublish
SO  - Biosci Trends. 2016 Sep 5;10(4):265-76. doi: 10.5582/bst.2016.01125. Epub 2016 
      Jul 26.