PMID- 27464669
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160916
LR  - 20181113
IS  - 2046-6390 (Print)
IS  - 2046-6390 (Electronic)
IS  - 2046-6390 (Linking)
VI  - 5
IP  - 9
DP  - 2016 Sep 15
TI  - Insulator speckles associated with long-distance chromatin contacts.
PG  - 1266-74
LID - 10.1242/bio.019455 [doi]
AB  - Nuclear foci of chromatin binding factors are, in many cases, discussed as sites 
      of long-range chromatin interaction in the three-dimensional nuclear space. 
      Insulator binding proteins have been shown to aggregate into insulator bodies, 
      which are large structures not involved in insulation; however, the more 
      diffusely distributed insulator speckles have not been analysed in this respect. 
      Furthermore, insulator binding proteins have been shown to drive binding sites 
      for Polycomb group proteins into Polycomb bodies. Here we find that insulator 
      speckles, marked by the insulator binding protein dCTCF, and Polycomb bodies show 
      differential association with the insulator protein CP190. They differ in number 
      and three-dimensional location with only 26% of the Polycomb bodies overlapping 
      with CP190. By using fluorescence in situ hybridization (FISH) probes to identify 
      long-range interaction (kissing) of the Hox gene clusters Antennapedia complex 
      (ANT-C) and Bithorax complex (BX-C), we found the frequency of interaction to be 
      very low. However, these rare kissing events were associated with insulator 
      speckles at a significantly shorter distance and an increased speckle number. 
      This suggests that insulator speckles are associated with long-distance 
      interaction.
CI  - (c) 2016. Published by The Company of Biologists Ltd.
FAU - Buxa, Melanie K
AU  - Buxa MK
AD  - Institute for Genetics, Justus-Liebig-University, Heinrich-Buff-Ring 58, Giessen 
      D35392, Germany.
FAU - Slotman, Johan A
AU  - Slotman JA
AD  - Department of Pathology, Josephine Nefkens Institute, Erasmus Optical Imaging 
      Centre, Erasmus MC, Postbus 2040, Rotterdam 3000 CA, The Netherlands.
FAU - van Royen, Martin E
AU  - van Royen ME
AD  - Department of Pathology, Josephine Nefkens Institute, Erasmus Optical Imaging 
      Centre, Erasmus MC, Postbus 2040, Rotterdam 3000 CA, The Netherlands.
FAU - Paul, Maarten W
AU  - Paul MW
AD  - Department of Pathology, Josephine Nefkens Institute, Erasmus Optical Imaging 
      Centre, Erasmus MC, Postbus 2040, Rotterdam 3000 CA, The Netherlands.
FAU - Houtsmuller, Adriaan B
AU  - Houtsmuller AB
AD  - Department of Pathology, Josephine Nefkens Institute, Erasmus Optical Imaging 
      Centre, Erasmus MC, Postbus 2040, Rotterdam 3000 CA, The Netherlands.
FAU - Renkawitz, Rainer
AU  - Renkawitz R
AUID- ORCID: 0000-0002-2591-1332
AD  - Institute for Genetics, Justus-Liebig-University, Heinrich-Buff-Ring 58, Giessen 
      D35392, Germany rainer.renkawitz@gen.bio.uni-giessen.de.
LA  - eng
PT  - Journal Article
DEP - 20160915
PL  - England
TA  - Biol Open
JT  - Biology open
JID - 101578018
PMC - PMC5051650
OTO - NOTNLM
OT  - CP190
OT  - Insulator
OT  - Polycomb bodies
OT  - Speckle
OT  - dCTCF
COIS- The authors declare no competing or financial interests.
EDAT- 2016/07/29 06:00
MHDA- 2016/07/29 06:01
PMCR- 2016/07/27
CRDT- 2016/07/29 06:00
PHST- 2016/07/29 06:00 [entrez]
PHST- 2016/07/29 06:00 [pubmed]
PHST- 2016/07/29 06:01 [medline]
PHST- 2016/07/27 00:00 [pmc-release]
AID - bio.019455 [pii]
AID - BIO019455 [pii]
AID - 10.1242/bio.019455 [doi]
PST - epublish
SO  - Biol Open. 2016 Sep 15;5(9):1266-74. doi: 10.1242/bio.019455.