PMID- 27465496
OWN - NLM
STAT- MEDLINE
DCOM- 20170428
LR  - 20181113
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 186
IP  - 2
DP  - 2016 Nov
TI  - Porphyromonas gingivalis infection exacerbates the onset of rheumatoid arthritis 
      in SKG mice.
PG  - 177-189
LID - 10.1111/cei.12847 [doi]
AB  - Epidemiological studies have linked periodontitis to rheumatoid arthritis (RA). 
      Porphyromonas gingivalis (Pg) was reported recently to produce citrullinated 
      protein (CP) and increase anti-cyclic CP antibody (ACPA), both of which have been 
      identified as causative factors of RA. In the present study, we determined the 
      effects of Pg infection on the exacerbation of RA in a mouse model. RA model mice 
      (SKG mice) were established by an intraperitoneal (i.p.) injection of laminarin 
      (LA). Mice were divided into six groups, Ctrl (PBS injection), LA (LA injection), 
      Pg/LA (Pg + LA injection), Pg (Pg injection), Ec/LA (Escherichia coli and LA 
      injection) and Ec (E. coli injection). In order to evaluate RA, joint swelling by 
      the arthritis score, bone morphology by microcomputed tomography (microCT), 
      haematoxylin and eosin staining, ACPA, matrix metalloproteinase-3 (MMP-3) and 
      cytokine level in serum by enzyme-linked immunosorbent assay were determined. 
      Osteoclast differentiation from bone marrow mononuclear cells (BMCs) was examined 
      to clarify the underlying mechanisms of RA. The presence of Pg and CP in joint 
      tissue was also investigated. The arthritis score was threefold higher in the 
      Pg/LA group than in the LA group. Severe bone destruction was observed in joint 
      tissue of the Pg/LA group. A microCT analysis of the Pg/LA group revealed a 
      decrease in bone density. ACPA, MMP-3, interleukin (IL)-2, IL-6, CXCL1 and 
      macrophage inflammatory protein (MIP)-1alpha levels from the Pg/LA group were the 
      highest. The osteoclastogenesis of BMCs was enhanced in the Pg/LA group. 
      Furthermore, large amounts of Pg components and CP were detected in the Pg/LA 
      group. In conclusion, Pg infection has the potential to exacerbate RA.
CI  - (c) 2016 British Society for Immunology.
FAU - Yamakawa, M
AU  - Yamakawa M
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Ouhara, K
AU  - Ouhara K
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University. 
      kouhara@hiroshima-u.ac.jp.
FAU - Kajiya, M
AU  - Kajiya M
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Munenaga, S
AU  - Munenaga S
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Kittaka, M
AU  - Kittaka M
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Yamasaki, S
AU  - Yamasaki S
AD  - Department of Clinical Immunology and Rheumatology, Hiroshima University 
      Hospital, Hiroshima, Japan.
FAU - Takeda, K
AU  - Takeda K
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Takeshita, K
AU  - Takeshita K
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Mizuno, N
AU  - Mizuno N
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Fujita, T
AU  - Fujita T
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
FAU - Sugiyama, E
AU  - Sugiyama E
AD  - Department of Clinical Immunology and Rheumatology, Hiroshima University 
      Hospital, Hiroshima, Japan.
FAU - Kurihara, H
AU  - Kurihara H
AD  - Department of Periodontal Medicine, Division of Applied Life Sciences, Institute 
      of Biomedical and Health Sciences, Hiroshima University.
LA  - eng
PT  - Journal Article
DEP - 20160829
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Ankle Joint/diagnostic imaging/pathology
MH  - Arthritis, Rheumatoid/diagnostic imaging/*etiology/*pathology
MH  - Bacteroidaceae Infections/*complications/*microbiology
MH  - Biomarkers
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Female
MH  - Gene Expression
MH  - Mice
MH  - Osteoclasts/cytology/metabolism
MH  - *Porphyromonas gingivalis
MH  - X-Ray Microtomography
PMC - PMC5054573
OTO - NOTNLM
OT  - Porphyromonas gingivalis
OT  - anti-CCP
OT  - citrullinated protein
OT  - periodontitis
OT  - rheumatoid arthritis
EDAT- 2016/07/29 06:00
MHDA- 2017/04/30 06:00
PMCR- 2017/11/01
CRDT- 2016/07/29 06:00
PHST- 2016/07/25 00:00 [accepted]
PHST- 2016/07/29 06:00 [pubmed]
PHST- 2017/04/30 06:00 [medline]
PHST- 2016/07/29 06:00 [entrez]
PHST- 2017/11/01 00:00 [pmc-release]
AID - CEI12847 [pii]
AID - 10.1111/cei.12847 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2016 Nov;186(2):177-189. doi: 10.1111/cei.12847. Epub 2016 Aug 
      29.