PMID- 27465867
OWN - NLM
STAT- MEDLINE
DCOM- 20170110
LR  - 20181202
IS  - 0529-567X (Print)
IS  - 0529-567X (Linking)
VI  - 51
IP  - 7
DP  - 2016 Jul 25
TI  - [Clinical analysis of preimplantation genetic diagnosis with HLA matching for 
      beta-thalassemia].
PG  - 491-7
LID - 10.3760/cma.j.issn.0529-567X.2016.07.003 [doi]
AB  - OBJECTIVE: To investigate the efficacy and feasibility of preimplantation genetic 
      diagnosis(PGD)with human leukocyte antigen(HLA)matching for beta-thalassemia. 
      METHODS: A total of 43 referred beta-thalassemia couples, with at least on child 
      in need of hematopoietic stem cell transplantation(HSCT), underwent PGD for HLA 
      matching at the First Affiliated Hospital of Sun Yat-sen University from 2010 to 
      2015. PGD cycles of these couples were retrospectively analyzed, and 15 infants 
      born from PGD-HLA were followed up. RESULTS: A total of 84 oocyte retrieval 
      cycles were performed, providing 14+/-7 oocytes per cycle. Fifty nine embryos 
      biopsied cycles were done, including 24 cleavage stage and 35 blastocyst stage 
      biopsy cycles. In cleavage stage, 259 embryos were biopsied, 93.4%(242/259)of 
      them with conclusive molecular diagnosis, and the percentage of unaffected 
      embryos(normo-homozygote and heterozygote)was 71.4%(185/259). The percentage of 
      HLA matched unaffected embryos was 9.3%(24/259). In blastocyst stage, 306 embryos 
      were biopsied, while 93.8%(287/306)of them were conclusive, and the percentage of 
      unaffected embryos was 70.6%(216/306). The percentage of HLA matched unaffected 
      embryos in blastocyst stage biopsy was 14.4%(44/306), which was higher than in 
      cleavage stage biopsy(P< 0.05). Forty three female carriers underwent 48 embryo 
      transfer cycles including 3 fresh and 45 frozen-thawed embryo transfer cycles. 
      Three fresh embryo transfer cycles were done after cleavage stage biopsy, 
      resulted in a birth of healthy twins born at 36 weeks' gestation. All the embryos 
      were frozen after blastocyst biopsied. Totally, 54 frozen-thawed embryos that 
      were transferred in 45 frozen-thawed embryo transfer cycles included 25 embryo 
      from cleavage stage biopsy and 29 embryo from blastocyst stage biopsy, and 42 of 
      them were HLA matched. Clinical pregnancy rate and implantation rate per cycle in 
      frozen-thawed embryo transfer were 38%(17/45)and 37%(20/54)respectively. A total 
      of 15 infants were born, 2 were from a fresh embryo transfer cycle, and 13 were 
      from frozen-thawed embryo transfer cycles. RESULTS of prenatal diagnosis from 
      delivered cases were matched to that of PGD. Four sick children have been cured 
      by HSCT from these HLA matched born siblings. CONCLUSION: PGD with HLA matching 
      is an established method for conceiving a child who may donate hematopoietic stem 
      cells to save an ill sibling.
FAU - Liu, X Y
AU  - Liu XY
AD  - Reproductive Medical Center, the First Affiliated Hospital of Sun Yat-sen 
      University, Guangzhou 510080, China.
FAU - Wang, J
AU  - Wang J
FAU - Zeng, Y H
AU  - Zeng YH
FAU - Ding, C H
AU  - Ding CH
FAU - Shen, X T
AU  - Shen XT
FAU - Zhou, W
AU  - Zhou W
FAU - Li, R
AU  - Li R
FAU - Zhou, C Q
AU  - Zhou CQ
FAU - Xu, Y W
AU  - Xu YW
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Fu Chan Ke Za Zhi
JT  - Zhonghua fu chan ke za zhi
JID - 16210370R
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Biopsy
MH  - Blastocyst
MH  - Embryo Implantation
MH  - *Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - Genetic Testing/*methods
MH  - HLA Antigens/*immunology
MH  - Heterozygote
MH  - Humans
MH  - Male
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - *Preimplantation Diagnosis
MH  - Prenatal Diagnosis
MH  - Retrospective Studies
MH  - Twins
MH  - beta-Thalassemia/diagnosis/*genetics
EDAT- 2016/07/29 06:00
MHDA- 2017/01/11 06:00
CRDT- 2016/07/29 06:00
PHST- 2016/07/29 06:00 [entrez]
PHST- 2016/07/29 06:00 [pubmed]
PHST- 2017/01/11 06:00 [medline]
AID - 10.3760/cma.j.issn.0529-567X.2016.07.003 [doi]
PST - ppublish
SO  - Zhonghua Fu Chan Ke Za Zhi. 2016 Jul 25;51(7):491-7. doi: 
      10.3760/cma.j.issn.0529-567X.2016.07.003.