PMID- 27466602 OWN - NLM STAT- MEDLINE DCOM- 20170627 LR - 20230216 IS - 1541-6100 (Electronic) IS - 0022-3166 (Print) IS - 0022-3166 (Linking) VI - 146 IP - 9 DP - 2016 Sep TI - Protein Supplementation Has Minimal Effects on Muscle Adaptations during Resistance Exercise Training in Young Men: A Double-Blind Randomized Clinical Trial. PG - 1660-9 LID - 10.3945/jn.116.231803 [doi] AB - BACKGROUND: To our knowledge the efficacy of soy-dairy protein blend (PB) supplementation with resistance exercise training (RET) has not been evaluated in a longitudinal study. OBJECTIVE: Our aim was to determine the effect of PB supplementation during RET on muscle adaptation. METHODS: In this double-blind randomized clinical trial, healthy young men [18-30 y; BMI (in kg/m(2)): 25 +/- 0.5] participated in supervised whole-body RET at 60-80% 1-repetition maximum (1-RM) for 3 d/wk for 12 wk with random assignment to daily receive 22 g PB (n = 23), whey protein (WP) isolate (n = 22), or an isocaloric maltodextrin (carbohydrate) placebo [(MDP) n = 23]. Serum testosterone, muscle strength, thigh muscle thickness (MT), myofiber cross-sectional area (mCSA), and lean body mass (LBM) were assessed before and after 6 and 12 wk of RET. RESULTS: All treatments increased LBM (P < 0.001). ANCOVA did not identify an overall treatment effect at 12 wk (P = 0.11). There tended to be a greater change in LBM from baseline to 12 wk in the PB group than in the MDP group (0.92 kg; 95% CI: -0.12, 1.95 kg; P = 0.09); however, changes in the WP and MDP groups did not differ. Pooling data from combined PB and WP treatments showed a trend for greater change in LBM from baseline to 12 wk compared with MDP treatment (0.69 kg; 95% CI: -0.08, 1.46 kg; P = 0.08). Muscle strength, mCSA, and MT increased (P < 0.05) similarly for all treatments and were not different (P > 0.10) between treatments. Testosterone was not altered. CONCLUSIONS: PB supplementation during 3 mo of RET tended to slightly enhance gains in whole-body and arm LBM, but not leg muscle mass, compared with RET without protein supplementation. Although protein supplementation minimally enhanced gains in LBM of healthy young men, there was no enhancement of gains in strength. This trial was registered at clinicaltrials.gov as NCT01749189. CI - (c) 2016 American Society for Nutrition. FAU - Reidy, Paul T AU - Reidy PT AD - Division of Rehabilitation Sciences; Departments of Nutrition and Metabolism. FAU - Borack, Michael S AU - Borack MS AD - Division of Rehabilitation Sciences; Departments of Nutrition and Metabolism. FAU - Markofski, Melissa M AU - Markofski MM AD - Sealy Center on Aging; and. FAU - Dickinson, Jared M AU - Dickinson JM AD - Division of Rehabilitation Sciences; Departments of Nutrition and Metabolism. FAU - Deer, Rachel R AU - Deer RR AD - Sealy Center on Aging; and. FAU - Husaini, Syed H AU - Husaini SH AD - Sealy Center on Aging; and. FAU - Walker, Dillon K AU - Walker DK AD - Division of Rehabilitation Sciences; Departments of Nutrition and Metabolism. FAU - Igbinigie, Sherry AU - Igbinigie S AD - School of Medicine, University of Texas Medical Branch, Galveston, TX; and. FAU - Robertson, Shay M AU - Robertson SM AD - Departments of Nutrition and Metabolism. FAU - Cope, Mark B AU - Cope MB AD - DuPont Nutrition & Health, St. Louis, MO. FAU - Mukherjea, Ratna AU - Mukherjea R AD - DuPont Nutrition & Health, St. Louis, MO. FAU - Hall-Porter, Janine M AU - Hall-Porter JM AD - DuPont Nutrition & Health, St. Louis, MO. FAU - Jennings, Kristofer AU - Jennings K AD - Preventive Medicine and Community Health, and. FAU - Volpi, Elena AU - Volpi E AD - Internal Medicine/Geriatrics; Sealy Center on Aging; and. FAU - Rasmussen, Blake B AU - Rasmussen BB AD - Division of Rehabilitation Sciences; Departments of Nutrition and Metabolism, Sealy Center on Aging; and blrasmus@utmb.edu. LA - eng SI - ClinicalTrials.gov/NCT01749189 GR - P30 AG024832/AG/NIA NIH HHS/United States GR - T32 HD007539/HD/NICHD NIH HHS/United States GR - UL1 TR001439/TR/NCATS NIH HHS/United States PT - Journal Article PT - Randomized Controlled Trial PT - Research Support, N.I.H., Extramural DEP - 20160727 PL - United States TA - J Nutr JT - The Journal of nutrition JID - 0404243 RN - 0 (Whey Proteins) RN - 3XMK78S47O (Testosterone) SB - IM MH - Adaptation, Physiological MH - Adolescent MH - Adult MH - Body Composition MH - Body Mass Index MH - Body Weight MH - *Dietary Supplements MH - Double-Blind Method MH - *Exercise MH - Humans MH - Male MH - Muscle Strength/drug effects MH - Muscle, Skeletal/*drug effects MH - *Resistance Training MH - Testosterone/blood MH - Whey Proteins/*administration & dosage MH - Young Adult PMC - PMC4997282 OTO - NOTNLM OT - animal proteins OT - function OT - milk proteins OT - muscle mass OT - plant protein OT - strength training OT - weight training COIS- 2 Author disclosures: PT Reidy, MS Borack, MM Markofski, JM Dickinson, RR Deer, SH Husaini, DK Walker, S Igbinigie, SM Robertson, MB Cope, R Mukherjea, JM Hall-Porter, K Jennings, E Volpi, and BB Rasmussen, no conflicts of interest. MB Cope and R Mukherjea were not involved with the research or laboratory analysis. Representatives from DuPont Nutrition & Health were not involved with data collection or laboratory analysis; however, JM Hall-Porter from DuPont Nutrition & Health was involved with the statistical analysis. EDAT- 2016/07/29 06:00 MHDA- 2017/06/28 06:00 PMCR- 2017/09/01 CRDT- 2016/07/29 06:00 PHST- 2016/02/19 00:00 [received] PHST- 2016/06/15 00:00 [accepted] PHST- 2016/07/29 06:00 [entrez] PHST- 2016/07/29 06:00 [pubmed] PHST- 2017/06/28 06:00 [medline] PHST- 2017/09/01 00:00 [pmc-release] AID - S0022-3166(23)00691-0 [pii] AID - 231803 [pii] AID - 10.3945/jn.116.231803 [doi] PST - ppublish SO - J Nutr. 2016 Sep;146(9):1660-9. doi: 10.3945/jn.116.231803. Epub 2016 Jul 27.