PMID- 27471359
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20160729
LR  - 20201001
IS  - 1011-6842 (Print)
IS  - 1011-6842 (Linking)
VI  - 32
IP  - 4
DP  - 2016 Jul
TI  - Gentiana scabra Reduces SR-A Expression and Oxidized-LDL Uptake in Human 
      Macrophages.
PG  - 460-6
AB  - BACKGROUND: Macrophages can imbibe low-density lipoprotein (LDL) through 
      scavenger receptors to become foam cells, which is critical in the initiation and 
      progression of atherosclerosis. Mounting evidence suggests that the 
      anti-inflammatory nature of Chinese herbs have the capacity to halt the complex 
      mechanisms underlying atherosclerosis. This study examined the effects of Chinese 
      herbs on foam cell formation. METHODS: Chinese herbs were obtained from the Sun 
      Ten pharmaceutic company. Using oxidized LDL (OxLDL) uptake and a cell toxicity 
      assay, we screened more than 30 types of Chinese herbs. Western blotting was used 
      to determine expressions of scavenger receptors (SRs) and 
      extracellular-signal-regulated kinase (ERK) activities. RESULTS: We found that 
      Gentiana scabra reduced oxidized LDL uptake effectively in THP-1 macrophages (p < 
      0.05 vs. OxLDL treated control). Moreover, treatment with Gentiana scabra in 
      THP-1 macrophages resulted in decreased expression of scavenger receptor- A 
      (SR-A) (p < 0.05 vs. control). Molecular investigation revealed that Gentiana 
      scabra inhibited SR-A protein expression, possibly by regulating ERK signaling 
      pathways (p < 0.05 vs. control). CONCLUSIONS: By regulating SR-A expression, 
      Gentiana scabra reduced oxidized LDL uptake in human macrophages. These results 
      support the potential use of Gentiana scabra in treating atherosclerosis.
FAU - Lin, Chin-Sheng
AU  - Lin CS
AD  - Division of Cardiology, Department of Medicine, Tri-Service General Hospital, 
      National Defense Medical Center, Taipei, Taiwan;
FAU - Liu, Pang-Yen
AU  - Liu PY
AD  - Division of Cardiology, Department of Medicine, Tri-Service General Hospital, 
      National Defense Medical Center, Taipei, Taiwan;
FAU - Lian, Chen-Hao
AU  - Lian CH
AD  - Division of General Laboratory, Ministry of Health and Welfare, KinMen Hospital, 
      Kinmen;
FAU - Lin, Ching-Heng
AU  - Lin CH
AD  - Best Health Chinese Medicine Clinic, Taipei;
FAU - Lai, Jenn-Haung
AU  - Lai JH
AD  - Division of Allergy, Immunology and Rheumatology, Department of Internal 
      Medicine, Chang Gung Memorial Hospital, Tao-Yuan;
FAU - Ho, Ling-Jun
AU  - Ho LJ
AD  - Institute of Cellular and System Medicine, National Health Research Institute, 
      Zhunan, Taiwan.
FAU - Yang, Shih-Ping
AU  - Yang SP
AD  - Division of Cardiology, Department of Medicine, Tri-Service General Hospital, 
      National Defense Medical Center, Taipei, Taiwan;
FAU - Cheng, Shu-Meng
AU  - Cheng SM
AD  - Division of Cardiology, Department of Medicine, Tri-Service General Hospital, 
      National Defense Medical Center, Taipei, Taiwan;
LA  - eng
PT  - Journal Article
PL  - China (Republic : 1949- )
TA  - Acta Cardiol Sin
JT  - Acta Cardiologica Sinica
JID - 101687085
PMC - PMC4963422
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Foam cells
OT  - Gentiana scabra
OT  - Macrophage
OT  - Oxidized LDL
EDAT- 2016/07/30 06:00
MHDA- 2016/07/30 06:01
PMCR- 2016/07/01
CRDT- 2016/07/30 06:00
PHST- 2016/07/30 06:00 [entrez]
PHST- 2016/07/30 06:00 [pubmed]
PHST- 2016/07/30 06:01 [medline]
PHST- 2016/07/01 00:00 [pmc-release]
AID - 10.6515/acs20150416a [doi]
PST - ppublish
SO  - Acta Cardiol Sin. 2016 Jul;32(4):460-6. doi: 10.6515/acs20150416a.