PMID- 27473784
OWN - NLM
STAT- MEDLINE
DCOM- 20171109
LR  - 20171120
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 65
DP  - 2016 Dec
TI  - The role of porcine reproductive and respiratory syndrome virus infection in 
      immune phenotype and Th1/Th2 balance of dendritic cells.
PG  - 245-252
LID - S0145-305X(16)30229-4 [pii]
LID - 10.1016/j.dci.2016.07.012 [doi]
AB  - The aim of this study was to characterize the immune response of dendritic cells 
      derived from monocytes (Mo-DCs) in the porcine peripheral blood following 
      infection with porcine reproductive and respiratory syndrome virus (PRRSV). Viral 
      load assays indicated that PRRSV efficiently infected Mo-DCs but failed to 
      replicate, whereas PRRSV infection of Mo-DCs decreased the expression of SLA-I, 
      SLA-II, CD80 and CD40 compared with those of mock Mo-DCs. Furthermore, we 
      analyzed the cytokine profiles using quantitative RT-PCR and ELISA. Results 
      indicated apparent changes in IL-10 and IL-12 p40 expression but not in IFN-gamma and 
      TNF-alpha among Mo-DCs infected with PRRSV and uninfected Mo-DCs. Additionally, flow 
      cytometry analysis of the altered Mo-DCs together with IL-4 and GM-CSF induction 
      for 7days revealed the typical morphology and phenotype with 91.73% purity before 
      infection with PRRSV. Overall, our data demonstrate that PRRSV impaired the 
      normal antigen presentation of Mo-DCs and led to inadequate adaptive immune 
      response by down-regulating the expression of SLA-I,SLA-II, CD80 and CD40. 
      Enhanced Th2 -type cytokine IL-10 secretion and reduced Th1-type cytokines 
      IL-12p40,IFN-gamma and TNF-alpha secretion results in Th1/Th2 imbalance.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Liu, Jinling
AU  - Liu J
AD  - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & 
      Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR 
      China.
FAU - Wei, Shu
AU  - Wei S
AD  - The Preventive Center of Animal Disease of LiaoNing Province, No.95, Renhe Road, 
      Shenbei District, Shenyang 110164, PR China.
FAU - Liu, Lixia
AU  - Liu L
AD  - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & 
      Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR 
      China.
FAU - Shan, Fengping
AU  - Shan F
AD  - Department of Immunology, Basic School of Medicine, China Medical University, No. 
      92, North Second Road, Shenyang 110001, PR China.
FAU - Zhao, Yujun
AU  - Zhao Y
AD  - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & 
      Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR 
      China.
FAU - Shen, Guoshun
AU  - Shen G
AD  - Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & 
      Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, PR 
      China. Electronic address: Shenguoshun@sohu.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160726
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Cytokines)
RN  - 0 (Histocompatibility Antigens)
SB  - IM
MH  - *Adaptive Immunity
MH  - Animals
MH  - Antigen Presentation
MH  - Arterivirus/*physiology
MH  - Arterivirus Infections/*immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/genetics/metabolism
MH  - Dendritic Cells/*immunology/virology
MH  - Histocompatibility Antigens/metabolism
MH  - Immune Evasion
MH  - Monocytes/*immunology
MH  - Swine
MH  - Th1-Th2 Balance
MH  - Transcriptome
MH  - Viral Load
OTO - NOTNLM
OT  - Dendritic cell
OT  - Immunophenotype
OT  - PRRSV
OT  - Thl /Th2
EDAT- 2016/07/31 06:00
MHDA- 2017/11/10 06:00
CRDT- 2016/07/31 06:00
PHST- 2016/02/23 00:00 [received]
PHST- 2016/07/19 00:00 [revised]
PHST- 2016/07/19 00:00 [accepted]
PHST- 2016/07/31 06:00 [entrez]
PHST- 2016/07/31 06:00 [pubmed]
PHST- 2017/11/10 06:00 [medline]
AID - S0145-305X(16)30229-4 [pii]
AID - 10.1016/j.dci.2016.07.012 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2016 Dec;65:245-252. doi: 10.1016/j.dci.2016.07.012. Epub 2016 
      Jul 26.