PMID- 27473999
OWN - NLM
STAT- MEDLINE
DCOM- 20170213
LR  - 20181202
IS  - 1532-2661 (Electronic)
IS  - 0034-5288 (Linking)
VI  - 107
DP  - 2016 Aug
TI  - Evaluation of protective immune response against fowl typhoid in chickens 
      vaccinated with the attenuated strain Salmonella Gallinarum DeltacobSDeltacbiA.
PG  - 220-227
LID - S0034-5288(16)30118-7 [pii]
LID - 10.1016/j.rvsc.2016.06.011 [doi]
AB  - Salmonella enterica serovar Gallinarum biovar Gallinarum (SG) causes fowl typhoid 
      in chickens, a septicemic infection which results in high mortality rates. This 
      disease causes high economic impact to the poultry industry worldwide because of 
      the mortality or elimination of positive flocks to control bacterial 
      dissemination. Live vaccines are used in the fields, however the characterization 
      of immune mechanisms important for protection are being studied to improve the 
      efficacy of vaccination schemes. In this study, we evaluated the immune response 
      in brown layer-hens, vaccinated or not, during the most critical period of 
      infection. Cellular and humoral immunity were extensively evaluated until 7 days 
      post-infection (DPI), by flow cytometry and ELISA, respectively. Furthermore, we 
      evaluated the expression of important pro-inflammatory cytokines after infection 
      of bone marrow derived macrophages (BMDMs) with the live attenuated SG vaccine 
      and with the wild SG strain. The results showed an increasing production of IgG 
      and IgM during the first week post-infection, in vaccinated layer-hens, which was 
      absent in unvaccinated birds. The population of CD8(+)CD44(+) and CD4(+)CD44(+) T 
      cells in spleen and cecal tonsils constantly decreased in unvaccinated birds in 
      comparison with vaccinated layers. The expression of IFN-gamma and TNF-alpha in BMDMs was 
      induced by both SG strains (attenuated and wild) at similar levels (p>0.05). 
      Vaccination with live SG vaccine reduced systemic infection by challenge strain 
      of SG and prevented the mortality rate of 85% that occurred in unvaccinated 
      layer-hens during 30 dpi. Furthermore, the immunization enhanced the 
      proliferation of effector CD4(+) and CD8(+) T cells after challenge.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Penha Filho, Rafael Antonio Casarin
AU  - Penha Filho RAC
AD  - School of Agricultural and Veterinary Sciences, Sao Paulo State University 
      (UNESP), Jaboticabal Campus, SP, Brazil, 14884-900; College of Veterinary 
      Medicine, Cornell University, Ithaca, NY, USA. Electronic address: 
      rafaelpenha12@yahoo.com.br.
FAU - Diaz, Silvia Juliana Acelas
AU  - Diaz SJA
AD  - School of Agricultural and Veterinary Sciences, Sao Paulo State University 
      (UNESP), Jaboticabal Campus, SP, Brazil, 14884-900.
FAU - Medina, Tiago da Silva
AU  - Medina TDS
AD  - Ribeirao Preto Medical School, University of Sao Paulo (USP), Ribeirao Preto, SP, 
      Brazil, 14049-900.
FAU - Chang, Yung-Fu
AU  - Chang YF
AD  - College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
FAU - da Silva, Joao Santana
AU  - da Silva JS
AD  - Ribeirao Preto Medical School, University of Sao Paulo (USP), Ribeirao Preto, SP, 
      Brazil, 14049-900.
FAU - Berchieri, Angelo Jr
AU  - Berchieri A Jr
AD  - School of Agricultural and Veterinary Sciences, Sao Paulo State University 
      (UNESP), Jaboticabal Campus, SP, Brazil, 14884-900.
LA  - eng
PT  - Journal Article
DEP - 20160621
PL  - England
TA  - Res Vet Sci
JT  - Research in veterinary science
JID - 0401300
RN  - 0 (Salmonella Vaccines)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vaccines, Attenuated)
SB  - IM
MH  - Animals
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - *Chickens/immunology
MH  - Female
MH  - Immunity, Humoral
MH  - Immunization
MH  - Poultry Diseases/microbiology/*prevention & control
MH  - Salmonella Infections, Animal/microbiology/*prevention & control
MH  - Salmonella Vaccines/*immunology
MH  - Salmonella enterica/immunology
MH  - Tumor Necrosis Factor-alpha
MH  - Vaccines, Attenuated/immunology
OTO - NOTNLM
OT  - BMDM
OT  - T cells
OT  - acquired immunity
OT  - flow cytometry
OT  - live vaccine
EDAT- 2016/07/31 06:00
MHDA- 2017/02/14 06:00
CRDT- 2016/07/31 06:00
PHST- 2015/12/12 00:00 [received]
PHST- 2016/05/20 00:00 [revised]
PHST- 2016/06/18 00:00 [accepted]
PHST- 2016/07/31 06:00 [entrez]
PHST- 2016/07/31 06:00 [pubmed]
PHST- 2017/02/14 06:00 [medline]
AID - S0034-5288(16)30118-7 [pii]
AID - 10.1016/j.rvsc.2016.06.011 [doi]
PST - ppublish
SO  - Res Vet Sci. 2016 Aug;107:220-227. doi: 10.1016/j.rvsc.2016.06.011. Epub 2016 Jun 
      21.