PMID- 27474616 OWN - NLM STAT- MEDLINE DCOM- 20180115 LR - 20180407 IS - 1879-1344 (Electronic) IS - 0144-8617 (Linking) VI - 151 DP - 2016 Oct 20 TI - Effect of oxidization and chitosan on the surface activity of soy protein isolate. PG - 700-706 LID - S0144-8617(16)30666-X [pii] LID - 10.1016/j.carbpol.2016.06.004 [doi] AB - The objective of this research was to study the effect of oxidization of performic acid and chitosan on the structure and surface properties of soy protein isolate. As the degree of oxidization increased, the emulsifying capacity and stability of all the oxidized soy protein isolate and chitosan (SPI/CHI) systems increased substantially, which were 29.7%, 31.7%, 34.1%, 31.9% and 31.9% respectively compared. Fluorescent spectrum showed that the fluorescence intensity of SPI/CHI conjugates decreased and the higher the oxidized degree was, the lower the fluorescence intensity. Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the location of acidic bands of SPI/CHI conjugates moved upwards and broadened. Meanwhile, the basic bands lightened or even disappeared gradually as the oxidization increased. Scanning electron microscope (SEM) showed that the particles became lager as the degree of oxidization increased. Better thermostability of the oxidized SPI/CHI systems was shown in the differential scanning calorimetry (DSC). CI - Copyright (c) 2016 Elsevier Ltd. All rights reserved. FAU - Wang, Wei AU - Wang W AD - Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: weiwangbiochem@163.com. FAU - Li, Junsheng AU - Li J AD - Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. FAU - Yan, Liujuan AU - Yan L AD - Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: 274187443@qq.com. FAU - Huang, Guoxia AU - Huang G AD - Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: 251493952@qq.com. FAU - Dong, Zhen AU - Dong Z AD - Faculty of Biological and Chemical Engineering, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China; Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Donghuan Road 268, Liuzhou, Guangxi 545006, PR China. Electronic address: 497257411@qq.com. LA - eng PT - Journal Article DEP - 20160603 PL - England TA - Carbohydr Polym JT - Carbohydrate polymers JID - 8307156 RN - 0 (Soybean Proteins) RN - 9012-76-4 (Chitosan) SB - IM MH - Chitosan/*chemistry MH - Oxidation-Reduction MH - Particle Size MH - Protein Stability MH - Soybean Proteins/*chemistry MH - Surface Properties MH - Temperature OTO - NOTNLM OT - Chitosan OT - Glycosylation OT - Oxidization OT - Performic acid OT - Soy protein EDAT- 2016/07/31 06:00 MHDA- 2018/01/16 06:00 CRDT- 2016/07/31 06:00 PHST- 2016/01/09 00:00 [received] PHST- 2016/05/21 00:00 [revised] PHST- 2016/06/01 00:00 [accepted] PHST- 2016/07/31 06:00 [entrez] PHST- 2016/07/31 06:00 [pubmed] PHST- 2018/01/16 06:00 [medline] AID - S0144-8617(16)30666-X [pii] AID - 10.1016/j.carbpol.2016.06.004 [doi] PST - ppublish SO - Carbohydr Polym. 2016 Oct 20;151:700-706. doi: 10.1016/j.carbpol.2016.06.004. Epub 2016 Jun 3.