PMID- 27475038
OWN - NLM
STAT- MEDLINE
DCOM- 20170602
LR  - 20170602
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1859
IP  - 10
DP  - 2016 Oct
TI  - Activation of Tag1 transposable elements in Arabidopsis dedifferentiating cells 
      and their regulation by CHROMOMETHYLASE 3-mediated CHG methylation.
PG  - 1289-98
LID - S1874-9399(16)30162-6 [pii]
LID - 10.1016/j.bbagrm.2016.07.012 [doi]
AB  - Dedifferentiation, that is, the acquisition of stem cell-like state, commonly 
      induced by stress (e.g., protoplasting), is characterized by open chromatin 
      conformation, a chromatin state that could lead to activation of transposable 
      elements (TEs). Here, we studied the activation of the Arabidopsis class II TE 
      Tag1, in which two copies, situated close to each other (near genes) on 
      chromosome 1 are found in Landsberg erecta (Ler) but not in Columbia (Col). We 
      first transformed protoplasts with a construct in which a truncated Tag1 (DeltaTag1 
      non-autonomous) blocks the expression of a reporter gene AtMBD5-GFP and found a 
      relatively high ectopic excision of DeltaTag1 accompanied by expression of AtMBD5-GFP 
      in protoplasts derived from Ler compared to Col; further increase was observed in 
      ddm1 (decrease in DNA methylation1) protoplasts (Ler background). Ectopic 
      excision was associated with transcription of the endogenous Tag1 and changes in 
      histone H3 methylation at the promoter region. Focusing on the endogenous Tag1 
      elements we found low level of excision in Ler protoplasts, which was slightly 
      and strongly enhanced in ddm1 and cmt3 (chromomethylase3) protoplasts, 
      respectively, concomitantly with reduction in Tag1 gene body (GB) CHG methylation 
      and increased Tag1 transcription; strong activation of Tag1 was also observed in 
      cmt3 leaves. Notably, in cmt3, but not in ddm1, Tag1 elements were excised out 
      from their original sites and transposed elsewhere in the genome. Our results 
      suggest that dedifferentiation is associated with Tag1 activation and that CMT3 
      rather than DDM1 plays a central role in restraining Tag1 activation via inducing 
      GB CHG methylation.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Khan, Asif
AU  - Khan A
AD  - French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob 
      Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 
      Midreshet Ben Gurion 84990, Israel.
FAU - Yadav, Narendra Singh
AU  - Yadav NS
AD  - French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob 
      Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 
      Midreshet Ben Gurion 84990, Israel.
FAU - Morgenstern, Yaakov
AU  - Morgenstern Y
AD  - French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob 
      Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 
      Midreshet Ben Gurion 84990, Israel.
FAU - Zemach, Assaf
AU  - Zemach A
AD  - Department of Molecular Biology and Ecology of Plants, Tel-Aviv University, 69978 
      Tel Aviv, Israel.
FAU - Grafi, Gideon
AU  - Grafi G
AD  - French Associates Institute for Agriculture and Biotechnology of Drylands, Jacob 
      Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, 
      Midreshet Ben Gurion 84990, Israel. Electronic address: ggrafi@bgu.ac.il.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160728
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (DDM1 protein, Arabidopsis)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Histones)
RN  - 0 (MBD5 protein, Arabidopsis)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - EC 2.1.1.- (DNA-Cytosine Methylases)
RN  - EC 2.1.1.37 (CMT3 protein, Arabidopsis)
RN  - EC 2.7.7.- (TAG1 transposase)
RN  - EC 2.7.7.- (Transposases)
SB  - IM
MH  - Arabidopsis/cytology/*genetics/metabolism
MH  - Arabidopsis Proteins/*genetics/metabolism
MH  - Cell Dedifferentiation
MH  - DNA Methylation
MH  - *DNA Transposable Elements
MH  - DNA-Binding Proteins/*genetics/metabolism
MH  - DNA-Cytosine Methylases/*genetics/metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Reporter
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Histones/genetics/metabolism
MH  - Plant Cells/metabolism
MH  - Plant Leaves/cytology/genetics/metabolism
MH  - Protoplasts/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Transcription Factors/*genetics/metabolism
MH  - Transcription, Genetic
MH  - Transformation, Genetic
MH  - Transposases/*genetics/metabolism
OTO - NOTNLM
OT  - Chromomethylase3 (CMT3)
OT  - DNA methylation
OT  - Decrease in DNA methylation 1 (DDM1)
OT  - Dedifferentiation
OT  - Protoplasts
OT  - TAG1
OT  - Transposable elements
EDAT- 2016/08/01 06:00
MHDA- 2017/06/03 06:00
CRDT- 2016/08/01 06:00
PHST- 2016/05/29 00:00 [received]
PHST- 2016/07/13 00:00 [revised]
PHST- 2016/07/25 00:00 [accepted]
PHST- 2016/08/01 06:00 [entrez]
PHST- 2016/08/01 06:00 [pubmed]
PHST- 2017/06/03 06:00 [medline]
AID - S1874-9399(16)30162-6 [pii]
AID - 10.1016/j.bbagrm.2016.07.012 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2016 Oct;1859(10):1289-98. doi: 
      10.1016/j.bbagrm.2016.07.012. Epub 2016 Jul 28.