PMID- 27476491
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20171127
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 478
IP  - 2
DP  - 2016 Sep 16
TI  - Indispensable role for mouse ELP3 in embryonic stem cell maintenance and early 
      development.
PG  - 631-6
LID - S0006-291X(16)31242-6 [pii]
LID - 10.1016/j.bbrc.2016.07.120 [doi]
AB  - ELP3, a core component of Elongator, has been implicated in translational 
      regulation via modification of tRNA at the wobble position. However, the precise 
      biological function of ELP3 in early mouse development has not yet been defined. 
      We here provide evidence that ELP3 plays crucial roles in mouse embryonic stem 
      cell (ESC) maintenance and early development. ELP3 was detected ubiquitously in 
      blastocysts and E10.5 embryos and shown to be increased during ESC 
      differentiation. Depletion of ELP3 in ESC led to aberrant cell cycle progression, 
      along with reduced expression of genes for pluripotency. Interestingly, our 
      analyses revealed that, although the mRNA levels of the genes related to cell 
      cycle were increased, protein levels were diminished in knockdown (KD) ESCs. The 
      data, therefore, suggest that ELP3 function is critical for translational 
      efficiency of the genes. Consistent with a proliferation defect in KD cells, Elp3 
      knockout (KO) embryos suffered from severe growth retardation and failed to 
      develop beyond E12.5. In conclusion, we have demonstrated that ELP3 plays an 
      indispensable role in ESC survival, differentiation and embryonic development in 
      mouse.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Yoo, Hyunjin
AU  - Yoo H
AD  - Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for 
      Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea.
FAU - Son, Dabin
AU  - Son D
AD  - Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for 
      Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea.
FAU - Jang, Young-Joo
AU  - Jang YJ
AD  - Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for 
      Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea.
FAU - Hong, Kwonho
AU  - Hong K
AD  - Department of Nanobiomedical Science & BK21 PLUS NBM Global Research Center for 
      Regenerative Medicine, Dankook University, Cheonan, 330-714, Republic of Korea. 
      Electronic address: kwonho.hong@dankook.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160729
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - EC 2.3.1.48 (Elp3 protein, mouse)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
SB  - IM
MH  - Animals
MH  - Cell Cycle
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Proliferation
MH  - Embryo Loss/genetics/metabolism
MH  - Embryo, Mammalian/embryology/metabolism
MH  - Fetal Growth Retardation/genetics/metabolism
MH  - *Gene Expression Regulation, Developmental
MH  - Gene Knockdown Techniques
MH  - Histone Acetyltransferases/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mouse Embryonic Stem Cells/*cytology/metabolism
OTO - NOTNLM
OT  - ELP3
OT  - Elongator
OT  - Embryonic stem cell
OT  - Mouse development
EDAT- 2016/08/02 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/08/02 06:00
PHST- 2016/06/07 00:00 [received]
PHST- 2016/07/28 00:00 [accepted]
PHST- 2016/08/02 06:00 [entrez]
PHST- 2016/08/02 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
AID - S0006-291X(16)31242-6 [pii]
AID - 10.1016/j.bbrc.2016.07.120 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Sep 16;478(2):631-6. doi: 
      10.1016/j.bbrc.2016.07.120. Epub 2016 Jul 29.