PMID- 27476725
OWN - NLM
STAT- MEDLINE
DCOM- 20180321
LR  - 20180321
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 571
DP  - 2016 Nov 15
TI  - Comparative liver accumulation of dioxin-like compounds in sheep and cattle: 
      Possible role of AhR-mediated xenobiotic metabolizing enzymes.
PG  - 1222-9
LID - S0048-9697(16)31602-3 [pii]
LID - 10.1016/j.scitotenv.2016.07.150 [doi]
AB  - PCDDs, PCDFs, and PCBs are persistent organic pollutants (POPs) that accumulate 
      in animal products and may pose serious health problems. Those able to bind the 
      aryl hydrocarbon receptor (AhR), eliciting a plethora of toxic responses, are 
      defined dioxin-like (DL) compounds, while the remainders are called non-DL (NDL). 
      An EFSA opinion has highlighted the tendency of ovine liver to specifically 
      accumulate DL-compounds to a greater extent than any other farmed ruminant 
      species. To examine the possible role in such an accumulation of xenobiotic 
      metabolizing enzymes (XME) involved in DL-compound biotransformation, liver 
      samples were collected from ewes and cows reared in an area known for low dioxin 
      contamination. A related paper reported that sheep livers had about 5-fold higher 
      DL-compound concentrations than cattle livers, while the content of the six 
      marker NDL-PCBs did not differ between species. Specimens from the same animals 
      were subjected to gene expression analysis for AhR, AhR nuclear translocator 
      (ARNT) and AhR-dependent oxidative and conjugative pathways; XME protein 
      expression and activities were also investigated. Both AhR and ARNT mRNA levels 
      were about 2-fold lower in ovine samples and the same occurred for CYP1A1 and 
      CYP1A2, being approximately 3- and 9-fold less expressed in sheep compared to 
      cattle, while CYP1B1 could be detectable in cattle only. The results of the 
      immunoblotting and catalytic activity (most notably EROD) measurements of the 
      CYP1A family enzymes were in line with the gene expression data. By contrast, 
      phase II enzyme expression and activities in sheep were higher (UGT1A) or similar 
      (GSTA1, NQO1) to those recorded in cattle. The overall low expression of CYP1 
      family enzymes in the sheep is in line with the observed liver accumulation of 
      DL-compounds and is expected to affect the kinetics and the dynamics of other 
      POPs such as many polycyclic aromatic hydrocarbons, as well as of toxins (e.g. 
      aflatoxins) or drugs (e.g. benzimidazole anthelmintics) known to be metabolized 
      by those enzymes.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Girolami, F
AU  - Girolami F
AD  - Department of Veterinary Sciences, University of Torino, Largo Braccini 2, 
      Grugliasco, Italy. Electronic address: flavia.girolami@unito.it.
FAU - Spalenza, V
AU  - Spalenza V
AD  - Department of Veterinary Sciences, University of Torino, Largo Braccini 2, 
      Grugliasco, Italy. Electronic address: veronica.spalenza@unito.it.
FAU - Benedetto, A
AU  - Benedetto A
AD  - Istituto Zooprofilattico di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 
      Torino, Italy. Electronic address: Alessandro.Benedetto@izsto.it.
FAU - Manzini, L
AU  - Manzini L
AD  - Department of Veterinary Sciences, University of Torino, Largo Braccini 2, 
      Grugliasco, Italy. Electronic address: livio.manzini@unito.it.
FAU - Badino, P
AU  - Badino P
AD  - Department of Veterinary Sciences, University of Torino, Largo Braccini 2, 
      Grugliasco, Italy. Electronic address: paola.badino@unito.it.
FAU - Abete, M C
AU  - Abete MC
AD  - Istituto Zooprofilattico di Piemonte, Liguria e Valle d'Aosta, Via Bologna 148, 
      Torino, Italy. Electronic address: MariaCesarina.Abete@izsto.it.
FAU - Nebbia, C
AU  - Nebbia C
AD  - Department of Veterinary Sciences, University of Torino, Largo Braccini 2, 
      Grugliasco, Italy. Electronic address: carlo.nebbia@unito.it.
LA  - eng
PT  - Journal Article
DEP - 20160729
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Dioxins and Dioxin-like Compounds)
RN  - 0 (Receptors, Aryl Hydrocarbon)
SB  - IM
MH  - Animals
MH  - Cattle/*metabolism
MH  - Dioxins and Dioxin-like Compounds/*metabolism
MH  - Female
MH  - Liver/*metabolism
MH  - Male
MH  - Receptors, Aryl Hydrocarbon/*genetics/metabolism
MH  - Sheep/*metabolism
OTO - NOTNLM
OT  - Cattle
OT  - Dioxin-like compounds
OT  - Liver
OT  - Sheep
OT  - Xenobiotic metabolizing enzymes
EDAT- 2016/08/02 06:00
MHDA- 2018/03/22 06:00
CRDT- 2016/08/02 06:00
PHST- 2016/05/18 00:00 [received]
PHST- 2016/07/21 00:00 [revised]
PHST- 2016/07/21 00:00 [accepted]
PHST- 2016/08/02 06:00 [entrez]
PHST- 2016/08/02 06:00 [pubmed]
PHST- 2018/03/22 06:00 [medline]
AID - S0048-9697(16)31602-3 [pii]
AID - 10.1016/j.scitotenv.2016.07.150 [doi]
PST - ppublish
SO  - Sci Total Environ. 2016 Nov 15;571:1222-9. doi: 10.1016/j.scitotenv.2016.07.150. 
      Epub 2016 Jul 29.