PMID- 27477784
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20180719
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 436
DP  - 2016 Nov 15
TI  - Mumps virus induces innate immune responses in mouse ovarian granulosa cells 
      through the activation of Toll-like receptor 2 and retinoic acid-inducible gene 
      I.
PG  - 183-94
LID - S0303-7207(16)30273-8 [pii]
LID - 10.1016/j.mce.2016.07.033 [doi]
AB  - Mumps virus (MuV) infection may lead to oophoritis and perturb ovarian function. 
      However, the mechanisms underlying the activation of innate immune responses to 
      MuV infection in the ovary have not been investigated. This study showed that 
      Toll-like receptor 2 (TLR2) and retinoic acid-inducible gene I (RIG-I) 
      cooperatively initiate innate immune responses to MuV infection in mouse ovarian 
      granulosa cells. Ovarian granulosa cells infected with MuV significantly produced 
      pro-inflammatory cytokines and chemokines, including interleukin-1beta (IL-1beta), 
      tumor necrosis factor alpha (TNF-alpha), monocyte chemotactic protein 1 (MCP-1), and type 
      1 interferons (IFN-alpha and IFN-beta). Knockdown of RIG-I significantly decreased 
      MuV-induced cytokine expression. TLR2 deficiency reduced the expression of IL-1beta, 
      TNF-alpha, and MCP-1 but did not affect the expression of IFN-alpha and IFN-beta in 
      granulosa cells after infection with MuV. Intraperitoneal injection of MuV 
      induced the ovarian innate immune responses in vivo, which suppressed estradiol 
      synthesis and induced granulosa cell apoptosis. The results provide novel 
      insights into the mechanisms underlying MuV-induced innate immune responses in 
      the mouse ovary.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Wang, Qing
AU  - Wang Q
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Wu, Han
AU  - Wu H
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Cheng, Lijing
AU  - Cheng L
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Yan, Keqin
AU  - Yan K
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Shi, Lili
AU  - Shi L
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Zhao, Xiang
AU  - Zhao X
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Jiang, Qian
AU  - Jiang Q
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Wang, Fei
AU  - Wang F
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Chen, Yongmei
AU  - Chen Y
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China.
FAU - Li, Qihan
AU  - Li Q
AD  - Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, 
      650118, China.
FAU - Han, Daishu
AU  - Han D
AD  - School of Basic Medicine, Peking Union Medical College, Institute of Basic 
      Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China. Electronic 
      address: dshan@ibms.pumc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160728
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Cytokines)
RN  - 0 (Interferon Regulatory Factor-3)
RN  - 0 (Irf3 protein, mouse)
RN  - 0 (NF-kappa B)
RN  - 0 (Toll-Like Receptor 2)
RN  - EC 3.6.1.- (Ddx58 protein, mouse)
RN  - EC 3.6.4.13 (DEAD Box Protein 58)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Cytokines/metabolism
MH  - DEAD Box Protein 58/*metabolism
MH  - Female
MH  - Granulosa Cells/*immunology/*virology
MH  - Humans
MH  - *Immunity, Innate
MH  - Interferon Regulatory Factor-3/metabolism
MH  - Mice, Inbred C57BL
MH  - Mumps/immunology/virology
MH  - Mumps virus/*physiology
MH  - NF-kappa B/metabolism
MH  - Toll-Like Receptor 2/*metabolism
OTO - NOTNLM
OT  - Innate immune response
OT  - Mumps virus
OT  - Ovarian granulosa cell
OT  - Pattern recognition receptor
EDAT- 2016/08/02 06:00
MHDA- 2017/11/29 06:00
CRDT- 2016/08/02 06:00
PHST- 2016/03/16 00:00 [received]
PHST- 2016/07/26 00:00 [revised]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/08/02 06:00 [entrez]
PHST- 2016/08/02 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
AID - S0303-7207(16)30273-8 [pii]
AID - 10.1016/j.mce.2016.07.033 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2016 Nov 15;436:183-94. doi: 10.1016/j.mce.2016.07.033. Epub 
      2016 Jul 28.