PMID- 27478274
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240325
IS  - 0044-8249 (Print)
IS  - 1521-3757 (Electronic)
IS  - 0044-8249 (Linking)
VI  - 128
IP  - 10
DP  - 2016 Mar 1
TI  - Insights into 6-Methylsalicylic Acid Bio-assembly by Using Chemical Probes.
PG  - 3524-3528
AB  - Chemical probes capable of reacting with KS (ketosynthase)-bound biosynthetic 
      intermediates were utilized for the investigation of the model type I iterative 
      polyketide synthase 6-methylsalicylic acid synthase (6-MSAS) in vivo and in 
      vitro. From the fermentation of fungal and bacterial 6-MSAS hosts in the presence 
      of chain termination probes, a full range of biosynthetic intermediates was 
      isolated and characterized for the first time. Meanwhile, in vitro studies of 
      recombinant 6-MSA synthases with both nonhydrolyzable and hydrolyzable substrate 
      mimics have provided additional insights into substrate recognition, providing 
      the basis for further exploration of the enzyme catalytic activities.
FAU - Parascandolo, James S
AU  - Parascandolo JS
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK.
FAU - Havemann, Judith
AU  - Havemann J
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK.
FAU - Potter, Helen K
AU  - Potter HK
AD  - Department of Chemistry University of Cambridge Lensfield Road Cambridge CB2 1EW 
      UK.
FAU - Huang, Fanglu
AU  - Huang F
AD  - Department of Chemistry University of Cambridge Lensfield Road Cambridge CB2 1EW 
      UK; Department of Biochemistry University of Cambridge 80 Tennis Court Road 
      Cambridge CB2 1GA UK.
FAU - Riva, Elena
AU  - Riva E
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK.
FAU - Connolly, Jack
AU  - Connolly J
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK; 
      School of Biosciences The University of Birmingham Birmingham B15 2TT UK.
FAU - Wilkening, Ina
AU  - Wilkening I
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK.
FAU - Song, Lijiang
AU  - Song L
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK.
FAU - Leadlay, Peter F
AU  - Leadlay PF
AD  - Department of Biochemistry University of Cambridge 80 Tennis Court Road Cambridge 
      CB2 1GA UK.
FAU - Tosin, Manuela
AU  - Tosin M
AD  - Department of Chemistry University of Warwick Library Road Coventry CV4 7AL UK.
LA  - eng
GR  - BB/J007250/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
DEP - 20160202
PL  - Germany
TA  - Angew Chem Weinheim Bergstr Ger
JT  - Angewandte Chemie (Weinheim an der Bergstrasse, Germany)
JID - 100955692
PMC - PMC4950124
OTO - NOTNLM
OT  - Chemische Sonden
OT  - Dehydratase
OT  - Iterative Polyketidkatalyse
EDAT- 2016/08/02 06:00
MHDA- 2016/08/02 06:01
PMCR- 2016/07/19
CRDT- 2016/08/02 06:00
PHST- 2015/09/26 00:00 [received]
PHST- 2015/11/19 00:00 [revised]
PHST- 2016/08/02 06:00 [entrez]
PHST- 2016/08/02 06:00 [pubmed]
PHST- 2016/08/02 06:01 [medline]
PHST- 2016/07/19 00:00 [pmc-release]
AID - ANGE201509038 [pii]
AID - 10.1002/ange.201509038 [doi]
PST - ppublish
SO  - Angew Chem Weinheim Bergstr Ger. 2016 Mar 1;128(10):3524-3528. doi: 
      10.1002/ange.201509038. Epub 2016 Feb 2.