PMID- 27478308
OWN - NLM
STAT- MEDLINE
DCOM- 20170605
LR  - 20181113
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2016
DP  - 2016
TI  - Serotonin-Exacerbated DSS-Induced Colitis Is Associated with Increase in MMP-3 
      and MMP-9 Expression in the Mouse Colon.
PG  - 5359768
LID - 10.1155/2016/5359768 [doi]
LID - 5359768
AB  - Background. 5-HT enhances dextran sulfate sodium- (DSS-) induced colitis and is 
      involved in inflammatory bowel disease (IBD). Matrix metalloproteinases (MMPs) 
      play roles in the process of intestinal inflammation. Aims. To examine whether 
      5-HT induces MMPs expression in mouse colon to enhance DSS-induced colitis. 
      Materials and Methods. C57BL/6J (B6) mice were treated with either low-dose 
      (1.0 mg/kg) or high-dose (2.0 mg/kg) 5-HT by enema, low-dose (1.0%) or high-dose 
      (2.5%) DSS, or combined low-dose (1.0%) DSS and (1.0 mg/kg) 5-HT. Mouse colitis 
      was analyzed. MMPs and tissue inhibitors of MMPs (TIMPs) mRNA were measured by 
      real-time quantitative RT-PCR in mouse colon and in human Caco-2 cells and 
      neutrophils. MMP-3 and MMP-9 protein levels were quantified from 
      immunohistochemistry (IHC) images of mouse colons. Results. 5-HT exacerbated 
      DSS-induced colitis, low-dose 5-HT induces both MMP-3 and MMP-9, and high-dose 
      5-HT only increased MMP-3 mRNA expression in mouse colon. Mouse colon MMP-3 and 
      MMP-9 protein levels were also elevated by 5-HT treatment. The MMP-2, TIMP-1, and 
      TIMP-2 mRNA levels were increased in the inflamed colon. 5-HT induced MMP-3 and 
      MMP-9 mRNA expression in Caco-2 and human neutrophils, respectively, in vitro. 
      Conclusion. 5-HT induced MMP-3 and MMP-9 expression in mouse colon; these 
      elevated MMPs may contribute to DSS-induced colitis.
FAU - Chen, Menglu
AU  - Chen M
AUID- ORCID: 0000-0002-1044-396X
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China; Department of Clinical Laboratory, The First Affiliated Hospital 
      and College of Clinical Medicine, Henan University of Science and Technology, 
      Luoyang 471003, China.
FAU - Gao, Lei
AU  - Gao L
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China.
FAU - Chen, Pan
AU  - Chen P
AUID- ORCID: 0000-0001-6562-5296
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China.
FAU - Feng, Dandan
AU  - Feng D
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China.
FAU - Jiang, Yalin
AU  - Jiang Y
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China.
FAU - Chang, Yongchao
AU  - Chang Y
AUID- ORCID: 0000-0001-9274-0812
AD  - Department of Clinical Laboratory, The First Affiliated Hospital and College of 
      Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, 
      China.
FAU - Jin, Jianjun
AU  - Jin J
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China.
FAU - Chu, Fong-Fong
AU  - Chu FF
AUID- ORCID: 0000-0002-0894-3033
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China; Department of Cancer Genetics and Epigenetics, Beckman Research 
      Institute of City of Hope, Duarte, CA 91010, USA.
FAU - Gao, Qiang
AU  - Gao Q
AUID- ORCID: 0000-0002-4159-7486
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital and 
      College of Clinical Medicine, Henan University of Science and Technology, Luoyang 
      471003, China; Department of Gastroenterology and Hepatology, Beijing 
      Rehabilitation Hospital of Capital Medical University, 1 Gongliao Road, 
      Shijingshan District, Beijing 100114, China.
LA  - eng
PT  - Journal Article
DEP - 20160705
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 333DO1RDJY (Serotonin)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Caco-2 Cells
MH  - Colitis/*chemically induced/*metabolism
MH  - Colon/drug effects/*metabolism
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Real-Time Polymerase Chain Reaction
MH  - Serotonin/*pharmacology
PMC - PMC4949340
EDAT- 2016/08/02 06:00
MHDA- 2017/06/06 06:00
PMCR- 2016/07/05
CRDT- 2016/08/02 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2016/04/24 00:00 [revised]
PHST- 2016/05/29 00:00 [accepted]
PHST- 2016/08/02 06:00 [entrez]
PHST- 2016/08/02 06:00 [pubmed]
PHST- 2017/06/06 06:00 [medline]
PHST- 2016/07/05 00:00 [pmc-release]
AID - 10.1155/2016/5359768 [doi]
PST - ppublish
SO  - Mediators Inflamm. 2016;2016:5359768. doi: 10.1155/2016/5359768. Epub 2016 Jul 5.