PMID- 27480927
OWN - NLM
STAT- MEDLINE
DCOM- 20170529
LR  - 20171127
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 478
IP  - 2
DP  - 2016 Sep 16
TI  - Crystal structure of the kinase domain of human protein tyrosine kinase 6 (PTK6) 
      at 2.33 A resolution.
PG  - 637-42
LID - S0006-291X(16)31243-8 [pii]
LID - 10.1016/j.bbrc.2016.07.121 [doi]
AB  - Human Protein tyrosine kinase 6 (PTK6) (EC:2.7.10.2), also known as the breast 
      tumor kinase (BRK), is an intracellular non-receptor Src-related tyrosine kinase 
      expressed in a majority of human breast tumors and breast cancer cell lines, but 
      its expression is low or completely absent in normal mammary glands. In the 
      recent past, several studies have suggested that PTK6 is a potential therapeutic 
      target in cancer. To understand its structural and functional properties, the 
      PTK6 kinase domain (PTK6-KD) gene was cloned, overexpressed in a baculo-insect 
      cell system, purified and crystallized at room temperature. X-ray diffraction 
      data to 2.33 A resolution was collected on a single PTK6-KD crystal, which 
      belonged to the triclinic space group P1. The Matthews coefficient calculation 
      suggested the presence of four protein molecules per asymmetric unit, with a 
      solvent content of  approximately 50%.The structure has been solved by molecular replacement 
      and crystal structure data submitted to the protein data bank under the accession 
      number 5D7V. This is the first report of apo PTK6-KD structure crystallized in 
      DFG-in and alphaC-helix-out conformation.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Thakur, Manish Kumar
AU  - Thakur MK
AD  - Department of Biochemistry, University of Mysore, Mysore, 570005, India; 
      Department of Structural Biology, Jubilant Biosys Ltd, Bangalore, 560022, India.
FAU - Kumar, Amit
AU  - Kumar A
AD  - Department of Structural Biology, Jubilant Biosys Ltd, Bangalore, 560022, India.
FAU - Birudukota, Swarnakumari
AU  - Birudukota S
AD  - Department of Structural Biology, Jubilant Biosys Ltd, Bangalore, 560022, India.
FAU - Swaminathan, Srinivasan
AU  - Swaminathan S
AD  - Department of Structural Biology, Jubilant Biosys Ltd, Bangalore, 560022, India.
FAU - Tyagi, Rajiv
AU  - Tyagi R
AD  - Department of Structural Biology, Jubilant Biosys Ltd, Bangalore, 560022, India.
FAU - Gosu, Ramachandraiah
AU  - Gosu R
AD  - Department of Biochemistry, University of Mysore, Mysore, 570005, India; 
      Department of Structural Biology, Jubilant Biosys Ltd, Bangalore, 560022, India. 
      Electronic address: ramachandraiah_gosu@jubilantbiosys.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160730
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (PTK6 protein, human)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Baculoviridae/genetics/metabolism
MH  - Cloning, Molecular
MH  - Crystallography, X-Ray
MH  - Enzyme Assays
MH  - Gene Expression
MH  - Humans
MH  - Kinetics
MH  - Models, Molecular
MH  - *Mutation
MH  - Neoplasm Proteins/*chemistry/genetics/metabolism
MH  - Protein Conformation, alpha-Helical
MH  - Protein Domains
MH  - Protein-Tyrosine Kinases/*chemistry/genetics/metabolism
MH  - Recombinant Proteins/chemistry/genetics/metabolism
MH  - Sequence Alignment
MH  - Sf9 Cells
MH  - Spodoptera
MH  - Structure-Activity Relationship
OTO - NOTNLM
OT  - BRK
OT  - Cancer
OT  - Kinase domain
OT  - Non-receptor tyrosine kinase
OT  - PTK6
OT  - X-ray crystal structure
EDAT- 2016/08/03 06:00
MHDA- 2017/05/30 06:00
CRDT- 2016/08/03 06:00
PHST- 2016/07/20 00:00 [received]
PHST- 2016/07/28 00:00 [accepted]
PHST- 2016/08/03 06:00 [entrez]
PHST- 2016/08/03 06:00 [pubmed]
PHST- 2017/05/30 06:00 [medline]
AID - S0006-291X(16)31243-8 [pii]
AID - 10.1016/j.bbrc.2016.07.121 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2016 Sep 16;478(2):637-42. doi: 
      10.1016/j.bbrc.2016.07.121. Epub 2016 Jul 30.