PMID- 27481284
OWN - NLM
STAT- MEDLINE
DCOM- 20170811
LR  - 20171226
IS  - 1521-6551 (Electronic)
IS  - 1521-6543 (Linking)
VI  - 68
IP  - 10
DP  - 2016 Oct
TI  - Sequential epitopes of Dermatophagoides farinae allergens identified using 
      peptide microarray-based immunoassay.
PG  - 792-8
LID - 10.1002/iub.1540 [doi]
AB  - House dust mites produce over 30 proteins proposed to induce immunoglobulin E 
      (IgE) antibody production in patients. Continued identification of IgE-binding 
      epitopes of these allergens is critical to advancing diagnosis and treatment of 
      allergic disease. To identify possible sequential IgE-binding epitopes of the 
      major- and mid-potency allergens from the house dust mite Dermatophagoides 
      farinae by peptide microarray-based immunoassay, nucleotide sequences of D. 
      farinae allergens (Der f) 1, 2, 4, 5, and 7 were used to generate overlapping 
      peptides covering the full protein sequences minus signal peptides. Short 
      peptides were printed onto microarray chips. Because asthma occurs as a symptom 
      of mite allergy more commonly among children than adults, the peptide chips were 
      exposed to sera pooled from six serum-positive pediatric patients with D. farinae 
      hypersensitivity and six serum-negative control children for screening sequential 
      IgE-binding epitopes by IgE immunolabeling. Higher-than-average immunolabel 
      signal intensity was observed for 21 short peptides in the serum-positive group 
      (P < 0.01). Due to sequence overlap, these 21 signals represented four fragments 
      of Der f 1 (amino acid positions 46-53, 71-78, 99-110, 179-186), three fragments 
      of Der f 2 (15-22, 80-89, 106-113), six fragments of Der f 4 (69-82, 107-116, 
      225-232, 261-268, 355-365, 483-496), one fragment of Der f 5 (102-109), and three 
      fragments of Der f 7 (32-39, 52-64, 100-107). These findings not only demonstrate 
      the utility of a peptide microarray immunoassay in identifying epitopes for these 
      allergens, but also provide a foundation for future exploration of specific 
      immunotherapies. (c) 2016 IUBMB Life, 68(10):792-798, 2016.
CI  - (c) 2016 International Union of Biochemistry and Molecular Biology.
FAU - Cui, Yubao
AU  - Cui Y
AD  - Department of Clinical Laboratory, The Third People's Hospital, Affiliated 
      Yancheng Hospital, School of Medicine, Southeast University, Yancheng 224001, 
      Jiangsu Province, People's Republic of China. ybcui1975@hotmail.Com.
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China. 
      ybcui1975@hotmail.Com.
FAU - Teng, Feixiang
AU  - Teng F
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China.
FAU - Yu, LiLi
AU  - Yu L
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China.
FAU - Zhou, Ying
AU  - Zhou Y
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China.
FAU - Wang, Nan
AU  - Wang N
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China.
FAU - Zhang, Chengbo
AU  - Zhang C
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China.
FAU - Yang, Li
AU  - Yang L
AD  - Department of Laboratory Medicine, Yancheng Health Vocational & Technical 
      College, Yancheng 224006, Jiangsu Province, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160801
PL  - England
TA  - IUBMB Life
JT  - IUBMB life
JID - 100888706
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Epitopes)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Dermatophagoides/chemistry/*immunology
MH  - Asthma/blood/*immunology
MH  - Binding Sites
MH  - Child
MH  - Child, Preschool
MH  - Epitope Mapping
MH  - Epitopes/immunology
MH  - Female
MH  - Humans
MH  - Immunoassay
MH  - Immunoglobulin E/blood/chemistry
MH  - Male
MH  - Protein Array Analysis
MH  - Pyroglyphidae/*immunology
OTO - NOTNLM
OT  - epitopes
OT  - immunoglobulin E (IgE)
OT  - mite allergens
OT  - peptide microarray
EDAT- 2016/08/03 06:00
MHDA- 2017/08/12 06:00
CRDT- 2016/08/03 06:00
PHST- 2016/06/02 00:00 [received]
PHST- 2016/07/21 00:00 [accepted]
PHST- 2016/08/03 06:00 [entrez]
PHST- 2016/08/03 06:00 [pubmed]
PHST- 2017/08/12 06:00 [medline]
AID - 10.1002/iub.1540 [doi]
PST - ppublish
SO  - IUBMB Life. 2016 Oct;68(10):792-8. doi: 10.1002/iub.1540. Epub 2016 Aug 1.