PMID- 27484116
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20170406
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 3
DP  - 2016 Sep
TI  - Peripheral KATP activation inhibits pain sensitization induced by skin/muscle 
      incision and retraction via the nuclear factor-kappaB/c-Jun N-terminal kinase 
      signaling pathway.
PG  - 2632-8
LID - 10.3892/mmr.2016.5546 [doi]
AB  - The aim of the current study was to assess the effect of pinacidil activation of 
      ATP‑sensitive potassium (KATP) channels prior to skin/muscle incision and 
      retraction (SMIR) surgery on peripheral and central sensitization, and 
      investigate molecular interferential targets for preventive analgesia. Male 
      Sprague-Dawley rats were randomly assigned to one of the following five groups: 
      Control, incision (sham surgery), incision plus retraction (SMIR) group, SMIR 
      plus pinacidil (pinacidil) group and the SMIR plus pyrrolidine dithiocarbamate 
      (PDTC) group. The rats in the pinacidil and PDTC groups were intraperitoneally 
      injected with pinacidil or PDTC, respectively, prior to the SMIR procedure. The 
      mechanical withdrawal threshold (MWT) was determined. Western blotting was 
      performed to detect the alterations in the subunits of the KATP channels, Kir6.1 
      and SUR2, levels of nuclear factor‑kappaB (NF‑kappaB) in the tissue around the incision 
      and c‑Jun N‑terminal kinase (JNK) in the spinal cord. There was a significant 
      increase observed in the levels of NF‑kappaB and JNK following SMIR surgery compared 
      with the control group, and a significant reduction in MWT and the levels of 
      Kir6.1 and SUR2. Additionally, intraperitoneal administration of pinacidil 
      inhibited the reduction in MWT, and Kir6.1 and SUR2 levels. SMIR was observed to 
      result in increases in the levels of NF‑kappaB and JNK. In addition, in the PDTC 
      group, the alterations in MWT, NF‑kappaB, JNK, Kir6.1 and SUR2 resulting from SMIR 
      were blocked. The results of the current study suggest that the deteriorations in 
      the microenvironment resulting from the SMIR procedure can induce peripheral and 
      central sensitization, and that the activation of peripheral KATP by pinacidil 
      prior to SMIR is able to inhibit peripheral and central sensitization via the 
      NF‑kappaB/JNK signaling pathway, thus resulting in preventive analgesia.
FAU - Qian, Li-Ping
AU  - Qian LP
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, P.R. China.
FAU - Shen, Shi-Ren
AU  - Shen SR
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, P.R. China.
FAU - Chen, Jun-Jie
AU  - Chen JJ
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, P.R. China.
FAU - Ji, Lu-Lu
AU  - Ji LL
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, P.R. China.
FAU - Cao, Su
AU  - Cao S
AD  - Department of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, 
      Jiangsu 226001, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160727
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (KATP Channels)
RN  - 0 (NF-kappa B)
RN  - 0 (Transcription Factor RelA)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - *Ion Channel Gating
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - KATP Channels/genetics/*metabolism
MH  - Male
MH  - NF-kappa B/*metabolism
MH  - *Pain Threshold
MH  - Rats
MH  - *Signal Transduction
MH  - Surgical Wound
MH  - Transcription Factor RelA/genetics/metabolism
MH  - Wounds and Injuries/genetics/metabolism
EDAT- 2016/08/04 06:00
MHDA- 2017/04/07 06:00
CRDT- 2016/08/04 06:00
PHST- 2015/04/02 00:00 [received]
PHST- 2016/02/15 00:00 [accepted]
PHST- 2016/08/04 06:00 [entrez]
PHST- 2016/08/04 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
AID - 10.3892/mmr.2016.5546 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Sep;14(3):2632-8. doi: 10.3892/mmr.2016.5546. Epub 2016 Jul 27.