PMID- 27484405
OWN - NLM
STAT- MEDLINE
DCOM- 20170406
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 14
IP  - 3
DP  - 2016 Sep
TI  - Semaphorin 3A-modified adipose-derived stem cell sheet may improve 
      osseointegration in a type 2 diabetes mellitus rat model.
PG  - 2449-56
LID - 10.3892/mmr.2016.5568 [doi]
AB  - Although titanium (Ti) implants are considered to be an optimal choice for the 
      replacement of missing teeth, it remains difficult to obtain sufficient 
      osseointegration in patients with type 2 diabetes mellitus (T2DM). The present 
      study aimed to investigate whether adipose-derived stem cells (ASCs) may be used 
      to improve Ti implant osseointegration in T2DM conditions with the addition of 
      semaphorin 3A (Sema3A), a recently identified osteoprotective protein. Cell 
      morphology was observed using a scanning electron microscope. Cell proliferation 
      was determined using Cell Counting Kit‑8. Osteogenic differentiation was 
      confirmed by the staining of alkaline phosphatase, collagen secretion and calcium 
      deposition. An in vivo evaluation was performed in the T2DM rat model, which was 
      induced by a high‑fat diet and a low‑dose streptozotocin intraperitoneal 
      injection. A Sema3A‑modified ASC sheet was wrapped around the Ti implant, which 
      was subsequently inserted into the tibia. The rats were then exposed to Sema3A 
      stimulation. The morphology and proliferation ability of ASCs remained unchanged; 
      however, their osteogenic differentiation ability was increased. Micro‑computed 
      tomography scanning and histological observations confirmed that formation of new 
      bone was improved with the use of the Sema3A-modified ASCs sheet. The present 
      study indicated that the Sema3A‑modified ASCs sheet may be used to improve 
      osseointegration under T2DM conditions.
FAU - Fang, Kaixiu
AU  - Fang K
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Song, Wen
AU  - Song W
AD  - Department of Prosthodontics, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Wang, Lifeng
AU  - Wang L
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Xu, Xiaoru
AU  - Xu X
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Tan, Naiwen
AU  - Tan N
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Zhang, Sijia
AU  - Zhang S
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Wei, Hongbo
AU  - Wei H
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
FAU - Song, Yingliang
AU  - Song Y
AD  - Department of Oral Implants, School of Stomatology, State Key Laboratory of 
      Military Stomatology, Fourth Military Medical University, Xi'an, Shaanxi 710032, 
      P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20160728
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Dental Implants)
RN  - 0 (Semaphorin-3A)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Dental Implants
MH  - Diabetes Mellitus, Type 2/genetics/*metabolism
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/drug effects
MH  - Male
MH  - Osseointegration/*drug effects/genetics
MH  - Osteogenesis/genetics
MH  - Rats
MH  - Semaphorin-3A/*pharmacology
MH  - Stem Cells/cytology/*drug effects/*metabolism
PMC - PMC4991673
EDAT- 2016/08/04 06:00
MHDA- 2017/04/07 06:00
PMCR- 2016/07/28
CRDT- 2016/08/04 06:00
PHST- 2015/08/25 00:00 [received]
PHST- 2016/07/04 00:00 [accepted]
PHST- 2016/08/04 06:00 [entrez]
PHST- 2016/08/04 06:00 [pubmed]
PHST- 2017/04/07 06:00 [medline]
PHST- 2016/07/28 00:00 [pmc-release]
AID - mmr-14-03-2449 [pii]
AID - 10.3892/mmr.2016.5568 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Sep;14(3):2449-56. doi: 10.3892/mmr.2016.5568. Epub 2016 Jul 
      28.