PMID- 27484619
OWN - NLM
STAT- MEDLINE
DCOM- 20180424
LR  - 20240210
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Aug 3
TI  - Macromolecular crowding explains overflow metabolism in cells.
PG  - 31007
LID - 10.1038/srep31007 [doi]
LID - 31007
AB  - Overflow metabolism is a metabolic phenotype of cells characterized by mixed 
      oxidative phosphorylation (OxPhos) and fermentative glycolysis in the presence of 
      oxygen. Recently, it was proposed that a combination of a protein allocation 
      constraint and a higher proteome fraction cost of energy generation by OxPhos 
      relative to fermentation form the basis of overflow metabolism in the bacterium, 
      Escherichia coli. However, we argue that the existence of a maximum or optimal 
      macromolecular density is another essential requirement. Here we re-evaluate our 
      previous theory of overflow metabolism based on molecular crowding following the 
      proteomic fractions formulation. We show that molecular crowding is a key factor 
      in explaining the switch from OxPhos to overflow metabolism.
FAU - Vazquez, Alexei
AU  - Vazquez A
AD  - Beatson Institute for Cancer Research, Glasgow, UK.
FAU - Oltvai, Zoltan N
AU  - Oltvai ZN
AD  - Departments of Pathology and Computational &Systems Biology, University of 
      Pittsburgh School of Medicine, Pittsburgh, PA, USA.
LA  - eng
GR  - 21140/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
DEP - 20160803
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
SB  - IM
MH  - *Biobehavioral Sciences
MH  - Escherichia coli/*metabolism
MH  - Glycolysis/*physiology
MH  - *Oxidative Phosphorylation
PMC - PMC4971534
EDAT- 2016/08/04 06:00
MHDA- 2018/04/25 06:00
PMCR- 2016/08/03
CRDT- 2016/08/04 06:00
PHST- 2016/04/20 00:00 [received]
PHST- 2016/07/11 00:00 [accepted]
PHST- 2016/08/04 06:00 [entrez]
PHST- 2016/08/04 06:00 [pubmed]
PHST- 2018/04/25 06:00 [medline]
PHST- 2016/08/03 00:00 [pmc-release]
AID - srep31007 [pii]
AID - 10.1038/srep31007 [doi]
PST - epublish
SO  - Sci Rep. 2016 Aug 3;6:31007. doi: 10.1038/srep31007.