PMID- 27484889
OWN - NLM
STAT- MEDLINE
DCOM- 20180306
LR  - 20210109
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Print)
IS  - 1865-1674 (Linking)
VI  - 64
IP  - 5
DP  - 2017 Oct
TI  - Development of a Novel Reverse Transcription Loop-Mediated Isothermal 
      Amplification Assay for the Rapid Detection of African Horse Sickness Virus.
PG  - 1579-1588
LID - 10.1111/tbed.12549 [doi]
AB  - African horse sickness (AHS) is a disease of equids caused by African Horse 
      Sickness Virus (AHSV) and is transmitted by Culicoides midges. AHS is endemic in 
      sub-Saharan Africa, but during the past century, outbreaks of significant 
      economic importance and elevated mortality have been recorded in Northern African 
      countries, the Iberian and Arabian Peninsula, the Middle East and the Indian 
      subcontinent. Effective control combines the application of early warning 
      systems, accurate laboratory diagnosis and reporting, animal movement 
      restrictions, suitable vaccination and surveillance programs, and the 
      coordination of all these measures by efficient veterinary services. Conventional 
      reverse-transcriptase (RT) PCR (RT-PCR) and real-time RT-PCR (rRT-PCR) assays 
      have improved the sensitivity and rapidity of diagnosing AHS, resulting in the 
      adoption of these methods as recommended tests by the World Organisation for 
      Animal Health (OIE). However, currently these assays are only performed within 
      laboratory settings; therefore, the development of field diagnostics for AHS 
      would improve the fast implementation of control policies. Loop-mediated 
      isothermal amplification (LAMP) is an isothermal, autocycling, 
      strand-displacement nucleic acid amplification technique which can be performed 
      in the field. LAMP assays are attractive molecular assays because they are simple 
      to use, rapid, portable and have sensitivity and specificity within the range of 
      rRT-PCR. This study describes the development of a novel RT-LAMP assay for the 
      detection of AHSV. The AHSV RT-LAMP assay has an analytical sensitivity of 96.1% 
      when considering an rRT-PCR cut-off value of C(T)  > 36, or 91.3% when no rRT-PCR 
      cut-off is applied. Diagnostic sensitivity and specificity were 100%. This assay 
      provides for a rapid and low cost AHS diagnostic for use in the field.
CI  - (c) 2016 The Authors. Transboundary and Emerging Diseases Published by Blackwell 
      Verlag GmbH.
FAU - Fowler, V L
AU  - Fowler VL
AD  - The Pirbright Institute, Pirbright, Woking, Surrey, UK.
FAU - Howson, E L A
AU  - Howson ELA
AD  - The Pirbright Institute, Pirbright, Woking, Surrey, UK.
FAU - Flannery, J
AU  - Flannery J
AD  - The Pirbright Institute, Pirbright, Woking, Surrey, UK.
FAU - Romito, M
AU  - Romito M
AD  - ARC-Onderstepoort Veterinary Institute, Onderstepoort, South Africa.
FAU - Lubisi, A
AU  - Lubisi A
AD  - ARC-Onderstepoort Veterinary Institute, Onderstepoort, South Africa.
FAU - Aguero, M
AU  - Aguero M
AD  - Laboratorio Central de Veterinaria-Sanidad Animal, Algete (Madrid), Spain.
FAU - Mertens, P
AU  - Mertens P
AD  - The Pirbright Institute, Pirbright, Woking, Surrey, UK.
FAU - Batten, C A
AU  - Batten CA
AD  - The Pirbright Institute, Pirbright, Woking, Surrey, UK.
FAU - Warren, H R
AU  - Warren HR
AD  - OptiGene Limited, Horsham, West Sussex, UK.
FAU - Castillo-Olivares, J
AU  - Castillo-Olivares J
AD  - The Pirbright Institute, Pirbright, Woking, Surrey, UK.
LA  - eng
GR  - BBS/E/I/00001455/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
DEP - 20160802
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
SB  - IM
MH  - African Horse Sickness/*diagnosis/virology
MH  - African Horse Sickness Virus/genetics/*isolation & purification
MH  - Animals
MH  - Ceratopogonidae/*virology
MH  - Horses
MH  - Nucleic Acid Amplification Techniques/methods/*veterinary
MH  - Sensitivity and Specificity
PMC - PMC5600106
OTO - NOTNLM
OT  - African horse sickness virus
OT  - RT-LAMP
OT  - diagnostics
OT  - pen side
OT  - rapid detection
EDAT- 2016/08/04 06:00
MHDA- 2018/03/07 06:00
PMCR- 2017/09/15
CRDT- 2016/08/04 06:00
PHST- 2016/05/10 00:00 [received]
PHST- 2016/08/04 06:00 [pubmed]
PHST- 2018/03/07 06:00 [medline]
PHST- 2016/08/04 06:00 [entrez]
PHST- 2017/09/15 00:00 [pmc-release]
AID - TBED12549 [pii]
AID - 10.1111/tbed.12549 [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2017 Oct;64(5):1579-1588. doi: 10.1111/tbed.12549. Epub 
      2016 Aug 2.