PMID- 27486690
OWN - NLM
STAT- MEDLINE
DCOM- 20170720
LR  - 20180320
IS  - 2168-6084 (Electronic)
IS  - 2168-6068 (Linking)
VI  - 152
IP  - 10
DP  - 2016 Oct 1
TI  - Proliferative Nodules vs Melanoma Arising in Giant Congenital Melanocytic Nevi 
      During Childhood.
PG  - 1147-1151
LID - 10.1001/jamadermatol.2016.2667 [doi]
AB  - IMPORTANCE: The differential diagnosis between proliferative nodules (PNs) and 
      melanoma arising in congenital melanocytic nevi (CMN) is crucial, as patients 
      with PNs most often experience no increased risk of melanoma with metastases and 
      death. OBJECTIVE: To analyze the utility of immunohistochemistry and fluorescence 
      in situ hybridization (FISH) in distinguishing PNs from childhood and adult-onset 
      melanoma arising in CMN. DESIGN, SETTING, AND PARTICIPANTS: A case series was 
      conducted from June 29, 1989, to November 12, 2009, of 13 children with PNs 
      arising in CMN in childhood and 5 children with melanomas arising in CMN in 
      childhood. Five patients with giant CMN with no nodules were included as negative 
      controls, and 6 patients with melanomas arising in CMN in adulthood were included 
      as positive controls. Follow-up ranged from 3 to 21 years in all children (mean, 
      9.9 years) and from 3 months to 7 years in adults. Specimens were selected for 
      immunohistochemistry and FISH. All histopathologic sections were reviewed by 2 
      dermatopathologists who examined all nodules arising at different ages in the 
      same patient and, in the case of melanoma, all locations. Data analysis was 
      performed from January 1, 2013, to January 31, 2015. MAIN OUTCOMES AND MEASURES: 
      The ability to distinguish melanoma from PN using immunohistochemistry and/or 
      FISH. RESULTS: Of the 13 patients (5 boys and 8 girls) with PNs present at birth, 
      all PNs were stable (mean follow-up, 9 years). Eight patients with PNs and 4 of 5 
      patients with childhood-onset melanoma showed homogeneous staining for HMB45, 
      while heterogeneous staining for HMB45 was seen in 3 of 6 patients with 
      adult-onset melanoma. Expression of p16 was strongly positive in most patients 
      with childhood-onset PNs (10 of 11 patients) and melanoma (all patients) but 
      negative in 4 patients with adult-onset melanoma. Patients with PNs and the 5 
      patients with childhood-onset melanoma had numerical chromosomal aberrations 
      never observed in the adjacent CMN. The 2 children with FISH-positive PNs are 
      melanoma free after 7 and 4 years. Only 1 patient with childhood-onset melanoma 
      had a FISH aberration compared with 4 patients with adult-onset melanoma. 
      CONCLUSIONS AND RELEVANCE: Immunohistochemistry and the 4-probe FISH melanoma 
      analysis are not useful for distinguishing PN from childhood-onset melanoma as 
      opposed to adult-onset melanoma. Numerical anomalies seen in PNs but not in the 
      adjacent CMN could be the result of a chromosomal segregation malfunction 
      resulting in the development of nodules.
FAU - Vergier, Beatrice
AU  - Vergier B
AD  - Department of Pathology and Molecular Pathology, Hopital Haut-Leveque, Centre 
      Hospitalier Universitaire Bordeaux, Pessac, France2Institut National de la Sante 
      et de la Recherche Medicale U1053-UMR, Bordeaux Research In Translational 
      Oncology, Bordeaux University, Bordeaux, France.
FAU - Laharanne, Elodie
AU  - Laharanne E
AD  - Department of Pathology and Molecular Pathology, Hopital Haut-Leveque, Centre 
      Hospitalier Universitaire Bordeaux, Pessac, France2Institut National de la Sante 
      et de la Recherche Medicale U1053-UMR, Bordeaux Research In Translational 
      Oncology, Bordeaux University, Bordeaux, France.
FAU - Prochazkova-Carlotti, Martina
AU  - Prochazkova-Carlotti M
AD  - Department of Pathology and Molecular Pathology, Hopital Haut-Leveque, Centre 
      Hospitalier Universitaire Bordeaux, Pessac, France2Institut National de la Sante 
      et de la Recherche Medicale U1053-UMR, Bordeaux Research In Translational 
      Oncology, Bordeaux University, Bordeaux, France.
FAU - de la Fouchardiere, Arnaud
AU  - de la Fouchardiere A
AD  - Department of Pathology, Centre Leon Berard, Lyon, France.
FAU - Merlio, Jean-Philippe
AU  - Merlio JP
AD  - Department of Pathology and Molecular Pathology, Hopital Haut-Leveque, Centre 
      Hospitalier Universitaire Bordeaux, Pessac, France2Institut National de la Sante 
      et de la Recherche Medicale U1053-UMR, Bordeaux Research In Translational 
      Oncology, Bordeaux University, Bordeaux, France.
FAU - Kadlub, Natacha
AU  - Kadlub N
AD  - Unit of Maxillofacial and Plastic Surgery, Necker-Enfants Malades Hospital, 
      Paris, France.
FAU - Avril, Marie-Francoise
AU  - Avril MF
AD  - Department of Dermatology, Cochin Hospital, Assistance Publique-Hopitaux de 
      Paris, Paris-Descartes-Sorbonne University, Paris, France.
FAU - Bodemer, Christine
AU  - Bodemer C
AD  - Department of Dermatology, Cochin Hospital, Assistance Publique-Hopitaux de 
      Paris, Paris-Descartes-Sorbonne University, Paris, France6Department of 
      Dermatology, Necker-Enfants Malades Hospital, Paris, France.
FAU - Lacoste, Caroline
AU  - Lacoste C
AD  - Department of Dermatology, Cochin Hospital, Assistance Publique-Hopitaux de 
      Paris, Paris-Descartes-Sorbonne University, Paris, France7Department of 
      Pathology, Necker-Enfants Malades Hospital, Paris, France.
FAU - Boralevi, Franck
AU  - Boralevi F
AD  - Department of Dermatology, Centre Hospitalier Universitaire Bordeaux, Bordeaux 
      University, Pessac, France.
FAU - Taieb, Alain
AU  - Taieb A
AD  - Department of Dermatology, Centre Hospitalier Universitaire Bordeaux, Bordeaux 
      University, Pessac, France.
FAU - Ezzedine, Khaled
AU  - Ezzedine K
AD  - Department of Dermatology, Centre Hospitalier Universitaire Bordeaux, Bordeaux 
      University, Pessac, France.
FAU - Fraitag, Sylvie
AU  - Fraitag S
AD  - Department of Dermatology, Cochin Hospital, Assistance Publique-Hopitaux de 
      Paris, Paris-Descartes-Sorbonne University, Paris, France7Department of 
      Pathology, Necker-Enfants Malades Hospital, Paris, France.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Multicenter Study
PL  - United States
TA  - JAMA Dermatol
JT  - JAMA dermatology
JID - 101589530
RN  - 0 (Biomarkers, Tumor)
RN  - Melanocytic nevus syndrome, congenital
SB  - IM
MH  - Adult
MH  - Biomarkers, Tumor/*metabolism
MH  - *Cell Proliferation
MH  - Cell Transformation, Neoplastic
MH  - Child
MH  - Child, Preschool
MH  - Diagnosis, Differential
MH  - Female
MH  - Follow-Up Studies
MH  - France
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Medical Records Systems, Computerized
MH  - Melanoma/metabolism/mortality/*pathology
MH  - Nevus, Intradermal/pathology
MH  - Nevus, Pigmented/metabolism/*pathology
MH  - Retrospective Studies
MH  - Skin Neoplasms/metabolism/mortality/*pathology
EDAT- 2016/08/04 06:00
MHDA- 2017/07/21 06:00
CRDT- 2016/08/04 06:00
PHST- 2016/08/04 06:00 [pubmed]
PHST- 2017/07/21 06:00 [medline]
PHST- 2016/08/04 06:00 [entrez]
AID - 2538520 [pii]
AID - 10.1001/jamadermatol.2016.2667 [doi]
PST - ppublish
SO  - JAMA Dermatol. 2016 Oct 1;152(10):1147-1151. doi: 10.1001/jamadermatol.2016.2667.