PMID- 27488499
OWN - NLM
STAT- MEDLINE
DCOM- 20180528
LR  - 20200730
IS  - 1559-1174 (Electronic)
IS  - 1535-1084 (Linking)
VI  - 19
IP  - 1
DP  - 2017 Mar
TI  - Identification of Novel SCIRR69-Interacting Proteins During ER Stress Using 
      SILAC-Immunoprecipitation Quantitative Proteomics Approach.
PG  - 81-93
LID - 10.1007/s12017-016-8431-9 [doi]
AB  - Spinal cord injury and regeneration-related protein #69 (SCIRR69),also known as 
      cAMP-responsive element-binding protein 3-like 2, belongs to the CREB/ATF family, 
      some members of which play significant roles in ER stress. However, it is still 
      not fully elucidated whether SCIRR69 involves in ER stress and its biochemical 
      and functional roles during ER stress. In this study, we firstly treated fetal 
      rat spinal cord neuron cells (SCN) and PC12 cells with ER stress activator 
      thapsigargin (TG) or tunicamycin (TM) and then detected the expression pattern of 
      SCIRR69 in response to ER stress at mRNA and protein levels using real-time PCR 
      assay and immunoblotting. Results showed that the expression pattern of SCIRR69 
      was largely consistent with those of ER stress marker (ATF6, BIP and CHOP) at 
      either mRNA level or protein level, implying that SCIRR69 may play important 
      roles in ER stress. Subsequently, we used stable isotope labeling by amino acids 
      in cell culture (SILAC)-immunoprecipitation quantitative proteomics to identify 
      interaction partners of SCIRR69 during TG-induced ER stress in PC12 cells and 
      found that transitional endoplasmic reticulum ATPase (TERA) and sideroflexin-1 
      (SFXN1) were potential SCIRR69-interacting proteins. The interaction between 
      SCIRR69 and TERA or SFXN1 was validated using co-immunoprecipitation. Those 
      results provide some clues for novel signaling nexuses that made by interactions 
      between SCIRR69 and TERA or SFXN1. Our findings may facilitate a better 
      understanding of the fundamental functions of SCIRR69 during ER stress.
FAU - Chen, Yujian
AU  - Chen Y
AD  - State Key Laboratory of Proteomics, Department of Neurobiology, Institute of 
      Basic Medical Sciences, Beijing, 100850, China.
FAU - Liu, Yong
AU  - Liu Y
AD  - State Key Laboratory of Proteomics, Department of Neurobiology, Institute of 
      Basic Medical Sciences, Beijing, 100850, China. liuyongxiao1225@hotmail.com.
FAU - Lin, Shide
AU  - Lin S
AD  - State Key Laboratory of Proteomics, Department of Neurobiology, Institute of 
      Basic Medical Sciences, Beijing, 100850, China.
AD  - Department of Spinal Cord Injury, the General Hospital of Jinan Military Command, 
      Jinan, 250031, China.
FAU - Yang, Shuguang
AU  - Yang S
AD  - State Key Laboratory of Proteomics, Department of Neurobiology, Institute of 
      Basic Medical Sciences, Beijing, 100850, China.
FAU - Que, Haiping
AU  - Que H
AD  - State Key Laboratory of Proteomics, Department of Neurobiology, Institute of 
      Basic Medical Sciences, Beijing, 100850, China.
FAU - Liu, Shaojun
AU  - Liu S
AD  - State Key Laboratory of Proteomics, Department of Neurobiology, Institute of 
      Basic Medical Sciences, Beijing, 100850, China. liusj@bmi.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20160803
PL  - United States
TA  - Neuromolecular Med
JT  - Neuromolecular medicine
JID - 101135365
RN  - 0 (Biomarkers)
RN  - 0 (Creb3l2 protein, rat)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transcription Factors)
RN  - 11089-65-9 (Tunicamycin)
RN  - 67526-95-8 (Thapsigargin)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Endoplasmic Reticulum Stress/drug effects/*physiology
MH  - Gene Expression Regulation/drug effects
MH  - Immunoprecipitation/*methods
MH  - PC12 Cells
MH  - Protein Interaction Mapping/*methods
MH  - Proteomics
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Recombinant Proteins/metabolism
MH  - Signal Transduction
MH  - Thapsigargin/pharmacology
MH  - Transcription Factors/*metabolism
MH  - Tunicamycin/pharmacology
OTO - NOTNLM
OT  - ER stress
OT  - Protein interaction
OT  - Quantitative proteomics
OT  - SCIRR69
OT  - SILAC
EDAT- 2016/08/05 06:00
MHDA- 2018/05/29 06:00
CRDT- 2016/08/05 06:00
PHST- 2016/04/15 00:00 [received]
PHST- 2016/07/29 00:00 [accepted]
PHST- 2016/08/05 06:00 [pubmed]
PHST- 2018/05/29 06:00 [medline]
PHST- 2016/08/05 06:00 [entrez]
AID - 10.1007/s12017-016-8431-9 [pii]
AID - 10.1007/s12017-016-8431-9 [doi]
PST - ppublish
SO  - Neuromolecular Med. 2017 Mar;19(1):81-93. doi: 10.1007/s12017-016-8431-9. Epub 
      2016 Aug 3.