PMID- 27490210
OWN - NLM
STAT- MEDLINE
DCOM- 20170213
LR  - 20181202
IS  - 1872-7077 (Electronic)
IS  - 1382-6689 (Linking)
VI  - 46
DP  - 2016 Sep
TI  - Effects of inducers of cytochrome P450s on enrofloxacin N-deethylation in crucian 
      carp Carassius auratus gibelio.
PG  - 188-193
LID - S1382-6689(16)30193-4 [pii]
LID - 10.1016/j.etap.2016.07.015 [doi]
AB  - In this study with crucian carp (Carassius auratus gibelio), the effect on 
      enrofloxacin (EF) and its metabolite ciprofloxacin (CF) and on the activity of 
      cytochrome P450 1A (CYP1A) and cytochrome P450 3A (CYP3A) was estimated following 
      the oral administration of rifampicin (RIF) (12mg/kg) and beta-naphthoflavone (BNF) 
      (12mg/kg), respectively. First, reversed-phase high-performance liquid 
      chromatography (RP-HPLC) was used to detect the pharmacokinetics of EF with 
      continual blood sampling. In RIF-treated, BNF-treated and control groups, the 
      value of the CmaxCF/CmaxEF ratio was 4.41, 0.81 and 0.95, and the corresponding 
      value of the AUC0-t-CF/AUC0-t-EF ratio was 3.69, 1.84 and 1.76, respectively. In 
      the RIF-treated, BNF-treated and control groups, the MRT values of EF were 26.57, 
      27.45 and 30.88h, and the corresponding values for CF were 5.79, 35.18 and 
      38.11h, respectively. Based on these results for crucian carp, the accumulation 
      and elimination of EF and CF in the RIF-treated group were more rapid than in 
      BNF-treated and control groups. Second, liver microsomes were pretreated with the 
      inducer of CYP1A for BNF and that of CYP3A for RIF, and then the enzymatic 
      activities of CYP1A and CYP3A were measured, respectively. The activities of 
      ethoxyresorufin-O-deethylation (EROD) and erythromycin-N-demethylation (ERND) 
      increased significantly (P<0.05) for CYP1A and CYP3A, respectively. However, in 
      further experiments on the formation of CF, the level of EF N-deethylation was 
      significantly induced by RIF and inhibited by ketoconazole (KTZ) for CYP3A but 
      had no influence for CYP1A, BNF and berberine chloride (BER). We concluded that 
      CYP3A might be responsible for the N-deethylation of EF and because of this 
      activity, could also serve as a toxicity biomarker in crucian carp.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Fu, Guihong
AU  - Fu G
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China.
FAU - Zhou, Chang
AU  - Zhou C
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China.
FAU - Wang, Yuan
AU  - Wang Y
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China.
FAU - Fang, Wenhong
AU  - Fang W
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China. 
      Electronic address: fwenhong@163.com.
FAU - Zhou, Junfang
AU  - Zhou J
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China.
FAU - Zhao, Shu
AU  - Zhao S
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China.
FAU - Ma, Licai
AU  - Ma L
AD  - East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Key Laboratory of East China Sea and Oceanic Fishery Resources Exploitation and 
      Utilization, Ministry of Agriculture, 300 Jungong Road, Shanghai, 200090, China.
LA  - eng
PT  - Journal Article
DEP - 20160726
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Cytochrome P-450 Enzyme Inducers)
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP3A)
RN  - VJT6J7R4TR (Rifampin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/blood/*pharmacokinetics
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Cytochrome P-450 CYP3A/metabolism
MH  - Cytochrome P-450 Enzyme Inducers/*pharmacology
MH  - Drug Interactions
MH  - Enrofloxacin
MH  - Fluoroquinolones/blood/*pharmacokinetics
MH  - Goldfish/blood/*metabolism
MH  - Microsomes, Liver/drug effects/*enzymology
MH  - Rifampin/pharmacology
MH  - beta-Naphthoflavone/pharmacology
OTO - NOTNLM
OT  - Ciprofloxacin
OT  - Cytochrome P450s
OT  - Enrofloxacin
OT  - N-Deethylation
EDAT- 2016/08/05 06:00
MHDA- 2017/02/14 06:00
CRDT- 2016/08/05 06:00
PHST- 2016/04/08 00:00 [received]
PHST- 2016/07/18 00:00 [revised]
PHST- 2016/07/23 00:00 [accepted]
PHST- 2016/08/05 06:00 [entrez]
PHST- 2016/08/05 06:00 [pubmed]
PHST- 2017/02/14 06:00 [medline]
AID - S1382-6689(16)30193-4 [pii]
AID - 10.1016/j.etap.2016.07.015 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2016 Sep;46:188-193. doi: 10.1016/j.etap.2016.07.015. 
      Epub 2016 Jul 26.