PMID- 27492123
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20171116
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 56
DP  - 2016 Sep
TI  - Pinocembrin attenuates lipopolysaccharide-induced inflammatory responses in Labeo 
      rohita macrophages via the suppression of the NF-kappaB signalling pathway.
PG  - 459-466
LID - S1050-4648(16)30473-9 [pii]
LID - 10.1016/j.fsi.2016.07.038 [doi]
AB  - Pinocembrin is a flavonoid that has been reported to exhibit various 
      pharmacological and biological activities including antimicrobial, antioxidant, 
      and anti-inflammatory. To explore the anti-inflammatory activity of pinocembrin 
      in a fish cell line, we investigated its ability to regulate the inflammatory 
      mediators elevated by lipopolysaccharide (LPS) in Labeo rohita head-kidney (HK) 
      macrophages. HK macrophages of L. rohita were treated with LPS (1 mug mL(-1)) in 
      the presence or absence of pinocembrin. We examined the inhibitory effect of 
      pinocembrin on LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) 
      production. The inhibitory effect of pinocembrin on nitric oxide synthase (iNOS) 
      and cyclooxygenase-2 (COX-2) was investigated by RT-PCR and western blot. The 
      effect of pinocembrin on pro-inflammatory cytokines (tumour necrosis factor alpha 
      (TNF-alpha) and interleukin-1beta (IL-1beta)) and anti-inflammatory cytokine IL-10 was 
      investigated by ELISA and RT-PCR. The phosphorylation of three mitogen activated 
      protein kinases (MAPKs) ERK, JNK, and p38 was analysed by western blot. 
      Pinocembrin inhibited LPS-induced productions of NO and PGE2, and also markedly 
      inhibited TNF-alpha, IL-1beta, iNOS, and COX-2 production in a concentration-dependent 
      manner. In addition, TNF-alpha and IL-1beta mRNA expression levels decreased 
      significantly, while IL-10 mRNA expression increased (P < 0.05) with pinocembrin 
      pre-treatment. RT-PCR and western blot analysis showed that pinocembrin decreased 
      both the mRNA and protein expression levels of LPS-induced iNOS and COX-2 in HK 
      macrophages. Pinocembrin suppressed the phosphorylation of MAPK in LPS-stimulated 
      HK macrophages. Further, pinocembrin significantly inhibited LPS-induced NF-kappaB 
      transcriptional activity via the attenuation of IkappaBalpha degradation. Taken together, 
      pinocembrin reduced the levels of pro-inflammatory mediators, such as iNOS, 
      COX-2, TNF-alpha, and IL-1beta, by inhibiting NF-kappaB activation via the suppression of 
      ERK and p38 phosphorylation, and by attenuating the degradation of IkappaBalpha. These 
      results suggest that pinocembrin is a potential novel candidate for the treatment 
      of inflammatory conditions in L. rohita macrophages.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Giri, Sib Sankar
AU  - Giri SS
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, South Korea; 
      Dept. of Zoology, Kundavai Nachiyar Government Arts College for Women 
      (Autonomous), Thanjavur, Tamil Nadu, India.
FAU - Sen, Shib Sankar
AU  - Sen SS
AD  - School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
FAU - Sukumaran, Venkatachalam
AU  - Sukumaran V
AD  - Dept. of Zoology, Kundavai Nachiyar Government Arts College for Women 
      (Autonomous), Thanjavur, Tamil Nadu, India. Electronic address: 
      drvsukumar@gmail.com.
FAU - Park, Se Chang
AU  - Park SC
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul, South Korea. 
      Electronic address: parksec@snu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20160802
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Flavanones)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 8T7C8CH791 (pinocembrin)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - *Cyprinidae
MH  - Fish Diseases/chemically induced/*prevention & control
MH  - Flavanones/*pharmacology
MH  - Inflammation/chemically induced/prevention & control/*veterinary
MH  - Lipopolysaccharides/physiology
MH  - Macrophages/immunology
MH  - NF-kappa B/genetics/metabolism
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Labeo rohita
OT  - Lipopolysaccharide
OT  - NF-kappaB signalling pathway
OT  - Pinocembrin
OT  - macrophages
EDAT- 2016/08/06 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/08/06 06:00
PHST- 2016/04/05 00:00 [received]
PHST- 2016/07/13 00:00 [revised]
PHST- 2016/07/31 00:00 [accepted]
PHST- 2016/08/06 06:00 [entrez]
PHST- 2016/08/06 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
AID - S1050-4648(16)30473-9 [pii]
AID - 10.1016/j.fsi.2016.07.038 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Sep;56:459-466. doi: 10.1016/j.fsi.2016.07.038. Epub 
      2016 Aug 2.