PMID- 27492854
OWN - NLM
STAT- MEDLINE
DCOM- 20180112
LR  - 20181113
IS  - 1476-5500 (Electronic)
IS  - 0929-1903 (Print)
IS  - 0929-1903 (Linking)
VI  - 23
IP  - 9
DP  - 2016 Sep
TI  - Overexpression of angiopoietin 2 promotes the formation of oral squamous cell 
      carcinoma by increasing epithelial-mesenchymal transition-induced angiogenesis.
PG  - 295-302
LID - 10.1038/cgt.2016.30 [doi]
AB  - Oral squamous cell carcinoma (OSCC) is the most common cancer of the head and 
      neck and is associated with a high rate of lymph node metastasis. The initial 
      step in the metastasis and transition of tumors is epithelial-mesenchymal 
      transition (EMT)-induced angiogenesis, which can be mediated by angiopoietin 2 
      (ANG2), a key regulatory factor in angiogenesis. In the present study, 
      immunohistochemistry and real-time quantitative reverse transcriptase (qRT-PCR) 
      were used to measure the expression of ANG2 in OSCC tissues. Plasmids encoding 
      ANG2 mRNA were used for increased ANG2 expression in the OSCC cell line TCA8113. 
      The short interfering RNA (siRNA)-targeting ANG2 mRNA sequences were used to 
      inhibit ANG2 expression in TCA8113 cells. Subsequently, transwell assays were 
      performed to examine the effects of ANG2 on TCA8113 cell migration and invasion. 
      Furthermore, in vivo assays were performed to assess the effect of ANG2 on tumor 
      growth. Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) 
      assays and immunohistochemistry were used to examine cell apoptosis and 
      angiogenesis in tumor tissues, respectively. Finally, western blot analysis was 
      performed to evaluate tumor formation-related proteins in OSCC tissues. We found 
      that protein expression of ANG2 was remarkably upregulated in OSCC tissues. 
      Overexpression of ANG2 increased the migration and invasion of TCA8113 cells by 
      regulating EMT. Further investigations showed that overexpression of ANG2 
      increased tumor growth in nude mice, and angiogenesis of OSCC tissues increased 
      in the presence of ANG2 overexpression. Overexpression of ANG2 also reduced cell 
      apoptosis in tumor tissue cells. Finally, we found that overexpression of ANG2 
      resulted in changes in the expression of tumor formation-related proteins 
      including vimentin, E-cadherin, Bim, PUMA, Bcl-2, Bax, Cyclin D1, PCNA and CD31. 
      Our findings show that ANG2 has an important role in the migration and invasion 
      of OSCC. More importantly, further investigations suggested that overexpression 
      of ANG2 might increase OSCC metastasis by promoting angiogenesis in nude mice. 
      This stimulatory effect could be achieved by inducing abnormal EMT and by 
      reducing apoptosis and increasing proliferation of cells.
FAU - Li, C
AU  - Li C
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - Li, Q
AU  - Li Q
AD  - Southwest Medical College, Luzhou, China.
FAU - Cai, Y
AU  - Cai Y
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - He, Y
AU  - He Y
AD  - Southwest Medical College, Luzhou, China.
FAU - Lan, X
AU  - Lan X
AD  - The Fifth People's Hospital of Chengdu, Chengdu, China.
FAU - Wang, W
AU  - Wang W
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - Liu, J
AU  - Liu J
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - Wang, S
AU  - Wang S
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - Zhu, G
AU  - Zhu G
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - Fan, J
AU  - Fan J
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
FAU - Zhou, Y
AU  - Zhou Y
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
AD  - Chengdu Medical College, Chengdu, China.
FAU - Sun, R
AU  - Sun R
AD  - Department of Head and Neck Surgery, Sichuan Cancer Hospital, Chengdu, China.
AD  - Chengdu Medical College, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20160805
PL  - England
TA  - Cancer Gene Ther
JT  - Cancer gene therapy
JID - 9432230
RN  - 0 (Angiopoietin-2)
SB  - IM
MH  - Angiopoietin-2/*genetics/metabolism
MH  - Animals
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/*pathology
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Disease Models, Animal
MH  - Epithelial-Mesenchymal Transition/*genetics
MH  - *Gene Expression
MH  - Heterografts
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Mouth Neoplasms/*genetics/metabolism/*pathology
MH  - Neovascularization, Pathologic/*genetics/metabolism
MH  - Tumor Burden/genetics
PMC - PMC5033983
EDAT- 2016/08/06 06:00
MHDA- 2018/01/13 06:00
PMCR- 2016/09/23
CRDT- 2016/08/06 06:00
PHST- 2016/03/17 00:00 [received]
PHST- 2016/06/22 00:00 [revised]
PHST- 2016/06/24 00:00 [accepted]
PHST- 2016/08/06 06:00 [entrez]
PHST- 2016/08/06 06:00 [pubmed]
PHST- 2018/01/13 06:00 [medline]
PHST- 2016/09/23 00:00 [pmc-release]
AID - cgt201630 [pii]
AID - 10.1038/cgt.2016.30 [doi]
PST - ppublish
SO  - Cancer Gene Ther. 2016 Sep;23(9):295-302. doi: 10.1038/cgt.2016.30. Epub 2016 Aug 
      5.