PMID- 27493075
OWN - NLM
STAT- MEDLINE
DCOM- 20170726
LR  - 20180416
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 630
DP  - 2016 Sep 6
TI  - Transgenic approach to express the channelrhodopsin 2 gene in arginine 
      vasopressin neurons of rats.
PG  - 194-198
LID - S0304-3940(16)30564-X [pii]
LID - 10.1016/j.neulet.2016.08.001 [doi]
AB  - Optogenetics provides a powerful tool to regulate neuronal activity by 
      light-sensitive ion channels such as channelrhodopsin 2 (ChR2). Arginine 
      vasopressin (AVP; also known as the anti-diuretic hormone) is a multifunctional 
      hormone which is synthesized in the magnocellular neurosecretory cells (MNCs) of 
      the hypothalamus. Here, we have generated a transgenic rat that expresses an 
      AVP-ChR2-enhanced green fluorescent protein (eGFP) fusion gene in the MNCs of the 
      hypothalamus. The eGFP fluorescence that indicates the expression of ChR2-eGFP 
      was observed in the supraoptic nucleus (SON) and in the magnocellular division of 
      the paraventricular nucleus (PVN) that is known to contain AVP-secreting neurons. 
      The eGFP fluorescence intensities in those nuclei and posterior pituitary were 
      markedly increased after chronic salt loading (2% NaCl in drinking water for 
      5days). ChR2-eGFP was localized mainly in the membrane of AVP-positive MNCs. 
      Whole-cell patch-clamp recordings were performed from single MNCs isolated from 
      the SON of the transgenic rats, and blue light evoked repetitive action 
      potentials. Our work provides for the first time an optogenetic approach to 
      selectively activate AVP neurons in the rat.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Ishii, Masahiro
AU  - Ishii M
AD  - Department of Physiology, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan; Department of Pediatrics, University of Occupational 
      and Environmental Health, Kitakyushu, 807-8555 Japan.
FAU - Hashimoto, Hirofumi
AU  - Hashimoto H
AD  - Department of Physiology, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan.
FAU - Ohkubo, Jun-Ichi
AU  - Ohkubo JI
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, University of 
      Occupational and Environmental Health, Kitakyushu, 807-8555 Japan.
FAU - Ohbuchi, Toyoaki
AU  - Ohbuchi T
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, University of 
      Occupational and Environmental Health, Kitakyushu, 807-8555 Japan.
FAU - Saito, Takeshi
AU  - Saito T
AD  - Department of Neurosurgery, School of Medicine, University of Occupational and 
      Environmental Health, Kitakyushu, 807-8555 Japan.
FAU - Maruyama, Takashi
AU  - Maruyama T
AD  - Department of Physiology, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan.
FAU - Yoshimura, Mitsuhiro
AU  - Yoshimura M
AD  - Department of Physiology, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan.
FAU - Yamamoto, Yukiyo
AU  - Yamamoto Y
AD  - Department of Pediatrics, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan.
FAU - Kusuhara, Koichi
AU  - Kusuhara K
AD  - Department of Pediatrics, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan.
FAU - Ueta, Yoichi
AU  - Ueta Y
AD  - Department of Physiology, University of Occupational and Environmental Health, 
      Kitakyushu, 807-8555 Japan. Electronic address: yoichi@med.uoeh-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20160801
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Channelrhodopsins)
RN  - 0 (enhanced green fluorescent protein)
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - Animals
MH  - Arginine Vasopressin/genetics/*metabolism
MH  - Channelrhodopsins
MH  - Female
MH  - Green Fluorescent Proteins/genetics
MH  - Male
MH  - Neurons/*metabolism/physiology
MH  - Optogenetics/*methods
MH  - Paraventricular Hypothalamic Nucleus/*metabolism
MH  - Pituitary Gland, Posterior/metabolism
MH  - Rats
MH  - Rats, Transgenic
MH  - Rats, Wistar
MH  - Sodium Chloride/administration & dosage
MH  - Supraoptic Nucleus/*metabolism/physiology
OTO - NOTNLM
OT  - Arginine vasopressin
OT  - Channelrhodopsin 2
OT  - Rat
OT  - Salt loading
OT  - Transgene
OT  - Whole-cell patch-clamp recording
EDAT- 2016/08/06 06:00
MHDA- 2017/07/27 06:00
CRDT- 2016/08/06 06:00
PHST- 2016/06/21 00:00 [received]
PHST- 2016/07/25 00:00 [revised]
PHST- 2016/08/01 00:00 [accepted]
PHST- 2016/08/06 06:00 [entrez]
PHST- 2016/08/06 06:00 [pubmed]
PHST- 2017/07/27 06:00 [medline]
AID - S0304-3940(16)30564-X [pii]
AID - 10.1016/j.neulet.2016.08.001 [doi]
PST - ppublish
SO  - Neurosci Lett. 2016 Sep 6;630:194-198. doi: 10.1016/j.neulet.2016.08.001. Epub 
      2016 Aug 1.