PMID- 27496194
OWN - NLM
STAT- MEDLINE
DCOM- 20170306
LR  - 20181113
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 53
IP  - 1
DP  - 2017 Jan
TI  - Murine Flt3 ligand-generated plasmacytoid and conventional dendritic cells 
      display functional differentiation in activation, inflammation, and antigen 
      presentation during BCG infection in vitro.
PG  - 67-76
LID - 10.1007/s11626-016-0076-3 [doi]
AB  - Dendritic cells (DCs) are composed of distinct subsets. Their immunologic 
      functions (especially in pathogenic infection, such as with mycobacteria) are 
      poorly understood, largely because of their rarity and difficulty of preparation. 
      We used the murine Fms-like tyrosine kinase 3 (Flt3) ligand to generate 
      conventional DCs (FL-cDCs) and plasmacytoid DCs (FL-pDCs) and further evaluated 
      their immunological responses to bacillus Calmette-Guerin (BCG) infection in 
      vitro. BCG cells were observed inside both FL-cDCs and FL-pDCs by confocal 
      microscopy, as confirmed by flow cytometric analysis showing a low infection rate 
      of approximately 6 %, which was similar to in vivo results. The CD40, CD80, CD86, 
      and MHC-II proteins were significantly upregulated in both FL-cDCs and -pDCs 
      beginning at 4 h post-BCG exposure. FL-pDCs secreted TNF-alpha and IL-6 earlier and 
      at significantly higher levels in the first 12 h following infection, but 
      demonstrated delayed and weak activation and maturation compared to FL-cDCs. 
      Although both subsets proved capable of presenting a mycobacterial antigen, 
      FL-pDCs exhibited weaker activity in this respect than did FL-cDCs. In summary, 
      the existence of FL-generated cDCs and pDCs imply functional differentiation in 
      activation, inflammation, and antigen presentation, although both cells types 
      participated extensively in the immune response to BCG infection.
FAU - Meng, Chuang
AU  - Meng C
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Xu, Zhengzhong
AU  - Xu Z
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Hu, Maozhi
AU  - Hu M
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China.
AD  - Testing Center, Yangzhou University, Yangzhou, Jiangsu, China.
FAU - Liu, Jiaying
AU  - Liu J
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Pan, Zhiming
AU  - Pan Z
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China.
FAU - Chen, Xiang
AU  - Chen X
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China. chenxiang@yzu.edu.cn.
FAU - Jiao, Xinan
AU  - Jiao X
AD  - Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention 
      and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 
      University, Yangzhou, Jiangsu, China. jiao@yzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160805
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Proteins)
RN  - 0 (flt3 ligand protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Antigen Presentation/drug effects/*immunology
MH  - Biomarkers/metabolism
MH  - *Cell Differentiation/drug effects
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/drug effects/*microbiology/*pathology
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescence
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Green Fluorescent Proteins/metabolism
MH  - Inflammation/microbiology/*pathology
MH  - Membrane Proteins/*metabolism
MH  - Mice, Inbred C57BL
MH  - Mycobacterium bovis/drug effects/*physiology
MH  - Phenotype
OTO - NOTNLM
OT  - BCG
OT  - Dendritic cells
OT  - Flt3 ligand
OT  - Immune response
OT  - In vitro
OT  - Subset
EDAT- 2016/08/09 06:00
MHDA- 2017/03/07 06:00
CRDT- 2016/08/07 06:00
PHST- 2016/05/25 00:00 [received]
PHST- 2016/07/22 00:00 [accepted]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2017/03/07 06:00 [medline]
PHST- 2016/08/07 06:00 [entrez]
AID - 10.1007/s11626-016-0076-3 [pii]
AID - 10.1007/s11626-016-0076-3 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2017 Jan;53(1):67-76. doi: 
      10.1007/s11626-016-0076-3. Epub 2016 Aug 5.