PMID- 27497046
OWN - NLM
STAT- MEDLINE
DCOM- 20170213
LR  - 20171024
IS  - 1532-0456 (Print)
IS  - 1532-0456 (Linking)
VI  - 190
DP  - 2016 Dec
TI  - Effects of stocking density on antioxidant status, metabolism and immune response 
      in juvenile turbot (Scophthalmus maximus).
PG  - 1-8
LID - S1532-0456(16)30089-8 [pii]
LID - 10.1016/j.cbpc.2016.07.007 [doi]
AB  - This study was designed to evaluate the physiological and immune responses of 
      juvenile turbot to stocking density. Turbot (average weight 185.4g) were reared 
      for 120days in a land based recirculating aquaculture system (RAS) under three 
      stocking densities: low density (LD, ~9.3-26.1kg/m(2), initial to final density), 
      medium density (MD, ~13.6-38.2kg/m(2)) and high density (HD, ~19.1-52.3kg/m(2)). 
      Fish were sampled at days 0, 40, 80 and 120 to obtain growth parameters and liver 
      tissues. No significant difference was detected in growth, biochemical parameters 
      and gene expression among the three densities until at the final sampling (day 
      120). At the end of this trial, fish reared in HD group showed lower specific 
      growth rate (SGR) and mean weight than those reared in LD and MD groups. 
      Similarly, oxidative stress and metabolism analyses represented that antioxidants 
      (superoxide dismutase (SOD), catalase (CAT), glutathione (GSH)) and metabolic 
      enzymes (glycerol-3-phosphate dehydrogenase (G3PDH) and glucose-6-phosphate 
      dehydrogenase (G6PDH)) clearly reduced in the liver of turbot reared in HD group. 
      The gene expression data showed that glutathione S-transferase (GST), cytochrome 
      P450 1A (CYP1A), heat shock protein 70 (HSP 70) and metallothionein (MT) mRNA 
      levels were significantly up-regulated, and lysozyme (LYS) and hepcidin (HAMP) 
      mRNA levels were significantly down-regulated in HD group on day 120. Overall, 
      our results indicate that overly high stocking density might block the activities 
      of metabolic and antioxidant enzymes, and cause physiological stress and 
      immunosuppression in turbot.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Liu, Baoliang
AU  - Liu B
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, 
      Qingdao 266071, China. Electronic address: liubl@ysfri.ac.cn.
FAU - Jia, Rui
AU  - Jia R
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Han, Cen
AU  - Han C
AD  - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, 
      China.
FAU - Huang, Bin
AU  - Huang B
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, 
      Qingdao 266071, China. Electronic address: huangbin@ysfri.ac.cn.
FAU - Lei, Ji-Lin
AU  - Lei JL
AD  - Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Science, 
      Qingdao 266071, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20160803
PL  - United States
TA  - Comp Biochem Physiol C Toxicol Pharmacol
JT  - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
JID - 100959500
RN  - 0 (Antioxidants)
RN  - 0 (Fish Proteins)
SB  - IM
MH  - Age Factors
MH  - *Animal Husbandry
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - *Energy Metabolism
MH  - Fish Proteins/genetics/metabolism
MH  - *Fisheries
MH  - Flatfishes/genetics/immunology/metabolism/*physiology
MH  - Gene Expression Regulation
MH  - *Immune Tolerance
MH  - Lipid Peroxidation
MH  - Liver/enzymology
MH  - Myocardium/enzymology
MH  - *Oxidative Stress
MH  - Population Density
MH  - Time Factors
MH  - Weight Gain
OTO - NOTNLM
OT  - Antioxidative enzymes
OT  - Gene expression
OT  - Metabolism
OT  - Scophthalmus maximus
OT  - Stocking density
EDAT- 2016/08/09 06:00
MHDA- 2017/02/14 06:00
CRDT- 2016/08/07 06:00
PHST- 2016/03/16 00:00 [received]
PHST- 2016/07/09 00:00 [revised]
PHST- 2016/07/30 00:00 [accepted]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2017/02/14 06:00 [medline]
PHST- 2016/08/07 06:00 [entrez]
AID - S1532-0456(16)30089-8 [pii]
AID - 10.1016/j.cbpc.2016.07.007 [doi]
PST - ppublish
SO  - Comp Biochem Physiol C Toxicol Pharmacol. 2016 Dec;190:1-8. doi: 
      10.1016/j.cbpc.2016.07.007. Epub 2016 Aug 3.