PMID- 27497390
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20181202
IS  - 1872-8464 (Electronic)
IS  - 0165-5876 (Print)
IS  - 0165-5876 (Linking)
VI  - 88
DP  - 2016 Sep
TI  - Morphological changes in the round window membrane associated with Haemophilus 
      influenzae-induced acute otitis media in the chinchilla.
PG  - 74-81
LID - S0165-5876(16)30197-5 [pii]
LID - 10.1016/j.ijporl.2016.06.049 [doi]
AB  - OBJECTIVE: The round window membrane (RWM) encloses the round window, the opening 
      into the scala tympani (ST) from the middle ear. During the course of acute 
      otitis media (AOM), structural changes of the RWM can occur that potentially 
      affect sound transmission into and out of the cochlea. The relationship between 
      such structural changes and conductive hearing loss during AOM has remained 
      unclear. The focus of the current study was to compare the thickness distribution 
      across the RWM surface between normal ears and those with AOM in the chinchilla. 
      We assessed the occurrence of AOM-associated histological changes in this 
      membrane compared to uninfected control animals after AOM of two relatively short 
      durations. MATERIAL AND METHODS: AOM was induced by transbullar injection of the 
      nontypeable Haemophilus influenzae strain 86-028NP into two groups of adult 
      chinchillas (n = 3 each). Bullae were obtained from the two infected groups, at 4 
      days or 8 days post challenge. Structures and thickness of these RWMs were 
      compared between the two infected treatment groups and to RWMs from uninfected 
      control animals (n = 3) at seven different RWM locations. RESULTS: RWM thickness 
      in infected chinchillas increased significantly at locations along the central 
      line on the 4th day post bacterial challenge compared to values found for 
      uninfected control animals. Lymphocyte infiltration and edema were the primary 
      contributors to these thickness increases. No significant further increases in 
      RWM thickness were observed when RWMs from chinchillas ears infected for 4 and 8 
      days were compared. Thickness and structural changes at the RWM lateral and 
      medial areas were less visually obvious and not statistically significant among 
      the three treatment groups. These latter RWM regions clearly were less affected 
      during AOM than the central areas. CONCLUSIONS: This histological study 
      establishes that H. influenzae-induced AOM causes significant acute changes in 
      chinchilla RWM structure that are characterized by region-specific increases in 
      thickness. Our new morphological findings comparing normal and diseased 
      chinchilla RWMs identify yet another biomechanical mechanism by which nontypeable 
      H. influenzae may contribute to hearing loss in AOM.
CI  - Copyright (c) 2016 Elsevier Ireland Ltd. All rights reserved.
FAU - Jiang, Shangyuan
AU  - Jiang S
AD  - School of Aerospace and Mechanical Engineering and Biomedical Engineering Center, 
      University of Oklahoma, Norman, OK, USA.
FAU - Seale, Thomas W
AU  - Seale TW
AD  - Department of Pediatrics, University of Oklahoma Health Sciences Center, Oklahoma 
      City, OK, USA.
FAU - Gan, Rong Z
AU  - Gan RZ
AD  - School of Aerospace and Mechanical Engineering and Biomedical Engineering Center, 
      University of Oklahoma, Norman, OK, USA. Electronic address: rgan@ou.edu.
LA  - eng
GR  - R01 DC011585/DC/NIDCD NIH HHS/United States
PT  - Journal Article
DEP - 20160630
PL  - Ireland
TA  - Int J Pediatr Otorhinolaryngol
JT  - International journal of pediatric otorhinolaryngology
JID - 8003603
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Case-Control Studies
MH  - Chinchilla
MH  - Edema/pathology
MH  - Haemophilus Infections/*pathology
MH  - Haemophilus influenzae
MH  - Lymphocytes/pathology
MH  - Otitis Media/*pathology
MH  - Round Window, Ear/*pathology
PMC - PMC4978205
MID - NIHMS801908
OTO - NOTNLM
OT  - Acute otitis media
OT  - Chinchilla
OT  - Haemophilus influenzae
OT  - Histology
OT  - Round window membrane
EDAT- 2016/08/09 06:00
MHDA- 2017/03/14 06:00
PMCR- 2017/09/01
CRDT- 2016/08/07 06:00
PHST- 2016/03/30 00:00 [received]
PHST- 2016/06/28 00:00 [revised]
PHST- 2016/06/28 00:00 [accepted]
PHST- 2016/08/07 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
PHST- 2017/09/01 00:00 [pmc-release]
AID - S0165-5876(16)30197-5 [pii]
AID - 10.1016/j.ijporl.2016.06.049 [doi]
PST - ppublish
SO  - Int J Pediatr Otorhinolaryngol. 2016 Sep;88:74-81. doi: 
      10.1016/j.ijporl.2016.06.049. Epub 2016 Jun 30.