PMID- 27497528
OWN - NLM
STAT- MEDLINE
DCOM- 20161227
LR  - 20161230
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 113
DP  - 2016 Dec
TI  - Combined treatment with chondroitinase ABC and treadmill rehabilitation for 
      chronic severe spinal cord injury in adult rats.
PG  - 37-47
LID - S0168-0102(16)30108-0 [pii]
LID - 10.1016/j.neures.2016.07.005 [doi]
AB  - There are more than 50 times the number of chronic-phase spinal cord injury (SCI) 
      patients than there are acute patients, and over half of all SCI patients are 
      severely disabled. However, research focusing on chronic severe contusional SCI 
      remains very rare. Here, we evaluated whether chondroitinase ABC (C-ABC), a 
      degradative enzyme directed against chondroitin sulfate proteoglycans (CSPGs), 
      and treadmill rehabilitation could exert synergistic therapeutic actions against 
      chronic severe contusional SCI. First, we induced severe contusional SCI in adult 
      rats, and administered C-ABC intrathecally at 6 weeks post-injury for a period of 
      one week. Next, we performed treadmill rehabilitation from weeks 6 to 14 after 
      SCI, for a total period of eight weeks. The initiation of treadmill 
      rehabilitation triggered slight recovery between weeks 6 and 9, whereas C-ABC 
      administration stimulated a third phase of recovery between weeks 12 and 14. 
      Histologically, the C-ABC-treated rats showed an increase in the transverse 
      residual tissue area and the extent of neuronal fiber regeneration at a site 
      caudal to the lesion epicenter, and regrowth of putatively regenerating 
      serotonergic fibers was significantly increased at the epicenter. We suggest 
      that, when combined with intensive rehabilitation, C-ABC may play a beneficial 
      role, even in severe and chronic SCI.
CI  - Copyright A(c) 2016 Elsevier Ireland Ltd and Japan Neuroscience Society. All rights 
      reserved.
FAU - Shinozaki, Munehisa
AU  - Shinozaki M
AD  - Department of Physiology, Keio University School of Medicine, Shinanomachi 35, 
      Shinjuku-ku, Tokyo 1608582, Japan.
FAU - Iwanami, Akio
AU  - Iwanami A
AD  - Department of Orthopedic Surgery, Keio University School of Medicine, 
      Shinanomachi 35, Shinjuku-ku, Tokyo 1608582, Japan.
FAU - Fujiyoshi, Kanehiro
AU  - Fujiyoshi K
AD  - Department of Orthopedic Surgery, Keio University School of Medicine, 
      Shinanomachi 35, Shinjuku-ku, Tokyo 1608582, Japan.
FAU - Tashiro, Syoichi
AU  - Tashiro S
AD  - Department of Rehabilitation, Keio University School of Medicine, Shinanomachi 
      35, Shinjuku-ku, Tokyo 1608582, Japan.
FAU - Kitamura, Kazuya
AU  - Kitamura K
AD  - Department of Orthopedic Surgery, Keio University School of Medicine, 
      Shinanomachi 35, Shinjuku-ku, Tokyo 1608582, Japan.
FAU - Shibata, Shinsuke
AU  - Shibata S
AD  - Department of Physiology, Keio University School of Medicine, Shinanomachi 35, 
      Shinjuku-ku, Tokyo 1608582, Japan.
FAU - Fujita, Hiroshi
AU  - Fujita H
AD  - Department of Glycoresearch, Central Research Laboratories, Seikagaku 
      Corporation, 1253, Tateno 3-chome, Higashiyamato-shi, Tokyo 2070021, Japan.
FAU - Nakamura, Masaya
AU  - Nakamura M
AD  - Department of Orthopedic Surgery, Keio University School of Medicine, 
      Shinanomachi 35, Shinjuku-ku, Tokyo 1608582, Japan. Electronic address: 
      masa@a8.keio.jp.
FAU - Okano, Hideyuki
AU  - Okano H
AD  - Department of Physiology, Keio University School of Medicine, Shinanomachi 35, 
      Shinjuku-ku, Tokyo 1608582, Japan. Electronic address: hidokano@a2.keio.jp.
LA  - eng
PT  - Journal Article
DEP - 20160804
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Chondroitin Sulfate Proteoglycans)
RN  - EC 4.2.2.20 (Chondroitin ABC Lyase)
SB  - IM
MH  - Animals
MH  - Axons/drug effects/physiology
MH  - Chondroitin ABC Lyase/*therapeutic use
MH  - Chondroitin Sulfate Proteoglycans/metabolism
MH  - Combined Modality Therapy
MH  - Female
MH  - Nerve Regeneration
MH  - *Physical Conditioning, Animal
MH  - Rats, Sprague-Dawley
MH  - Spinal Cord Injuries/*drug therapy/physiopathology/*rehabilitation
OTO - NOTNLM
OT  - Axonal regeneration
OT  - Chondroitin sulfate proteoglycan
OT  - Glial scar
OT  - Neural plasticity
EDAT- 2016/08/09 06:00
MHDA- 2016/12/28 06:00
CRDT- 2016/08/08 06:00
PHST- 2016/05/18 00:00 [received]
PHST- 2016/07/20 00:00 [revised]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2016/12/28 06:00 [medline]
PHST- 2016/08/08 06:00 [entrez]
AID - S0168-0102(16)30108-0 [pii]
AID - 10.1016/j.neures.2016.07.005 [doi]
PST - ppublish
SO  - Neurosci Res. 2016 Dec;113:37-47. doi: 10.1016/j.neures.2016.07.005. Epub 2016 
      Aug 4.