PMID- 27497699
OWN - NLM
STAT- MEDLINE
DCOM- 20170718
LR  - 20181202
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 18
IP  - 10
DP  - 2016 Oct
TI  - Overexpression of tropomyosin receptor kinase A improves the survival and 
      Schwann-like cell differentiation of bone marrow stromal cells in nerve grafts 
      for bridging rat sciatic nerve defects.
PG  - 1256-69
LID - S1465-3249(16)30449-2 [pii]
LID - 10.1016/j.jcyt.2016.06.015 [doi]
AB  - BACKGROUND AIMS: Bone marrow stromal cells (BMSCs) can differentiate into 
      Schwann-like cells in vivo and effectively promote nerve regeneration and 
      functional recovery as the seed cells for peripheral nerve repair. However, the 
      survival rate and neural differentiation rate of the transplanted BMSCs are very 
      low, which would limit their efficacy. METHODS: In this work, rat BMSCs were 
      infected by recombinant lentiviruses to construct tropomyosin receptor kinase A 
      (TrkA)-overexpressing BMSCs and TrkA-shRNA-expressing BMSCs, which were then used 
      in transplantation for rat sciatic nerve defects. RESULTS: We showed that 
      lentivirus-mediated overexpression of TrkA in BMSCs can promote cell survival and 
      protect against serum-starve-induced apoptosis in vitro. At 8 weeks after 
      transplantation, the Schwann-like differentiated ratio of the existing implanted 
      cells had reached 74.8 +/- 1.6% in TrkA-overexpressing BMSCs-laden nerve grafts, 
      while 40.7 +/- 2.3% and 42.3 +/- 1.5% in vector and control BMSCs-laden nerve grafts, 
      but only 8.2 +/- 1.8% in TrkA-shRNA-expressing BMSCs-laden nerve grafts. The cell 
      apoptosis ratio of the existing implanted cells in TrkA-overexpressing 
      BMSCs-laden nerve grafts was 16.5 +/- 1.2%, while 33.9 +/- 1.9% and 42.6 +/- 2.9% in 
      vector and control BMSCs-laden nerve grafts, but 87.2 +/- 2.5% in 
      TrkA-shRNA-expressing BMSCs-laden nerve grafts. CONCLUSIONS: These results 
      demonstrate that TrkA overexpression can improve the survival and Schwann-like 
      cell differentiation of BMSCs and prevent cell death in nerve grafts, which may 
      have potential implication in advancing cell transplantation for peripheral nerve 
      repair.
CI  - Copyright (c) 2016 International Society for Cellular Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Zheng, Meige
AU  - Zheng M
AD  - Department of Orthopedic and Microsurgery, The First Affiliated Hospital of Sun 
      Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou, China; Key Laboratory of 
      Optoelectronic Devices and Systems of Ministry of Education and Guangdong 
      Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, 
      China.
FAU - Duan, Junxiu
AU  - Duan J
AD  - School of Medicine, Shenzhen University, Shenzhen, China.
FAU - He, Zhendan
AU  - He Z
AD  - School of Medicine, Shenzhen University, Shenzhen, China.
FAU - Wang, Zhiwei
AU  - Wang Z
AD  - Department of Neurology, Shenzhen Shekou People's Hospital, Shenzhen, China.
FAU - Mu, Shuhua
AU  - Mu S
AD  - Psychology & Social College, Shenzhen University, Shenzhen, China.
FAU - Zeng, Zhiwen
AU  - Zeng Z
AD  - School of Medicine, Shenzhen University, Shenzhen, China.
FAU - Qu, Junle
AU  - Qu J
AD  - Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and 
      Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, 
      Shenzhen, China.
FAU - Zhang, Jian
AU  - Zhang J
AD  - School of Medicine, Shenzhen University, Shenzhen, China. Electronic address: 
      jzhanghappy@163.com.
FAU - Wang, Dong
AU  - Wang D
AD  - Department of Orthopedic and Microsurgery, The First Affiliated Hospital of Sun 
      Yat-Sen University, No. 58 Zhongshan Road 2, Guangzhou, China. Electronic 
      address: david74429@126.com.
LA  - eng
PT  - Journal Article
DEP - 20160803
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - EC 2.7.10.1 (Receptor, trkA)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*physiology
MH  - Cell Differentiation/*genetics
MH  - Cell Survival/genetics
MH  - Cells, Cultured
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*physiology
MH  - Nerve Regeneration/*genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptor, trkA/*genetics/metabolism
MH  - Schwann Cells/cytology/*physiology
MH  - Sciatic Nerve/pathology/*physiology
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - BMSCs
OT  - Schwann-like cell differentiation
OT  - TrkA overexpression
OT  - nerve transplantation
OT  - shRNA interfere
OT  - survival
EDAT- 2016/08/09 06:00
MHDA- 2017/07/19 06:00
CRDT- 2016/08/08 06:00
PHST- 2016/05/24 00:00 [received]
PHST- 2016/06/28 00:00 [accepted]
PHST- 2016/08/08 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2017/07/19 06:00 [medline]
AID - S1465-3249(16)30449-2 [pii]
AID - 10.1016/j.jcyt.2016.06.015 [doi]
PST - ppublish
SO  - Cytotherapy. 2016 Oct;18(10):1256-69. doi: 10.1016/j.jcyt.2016.06.015. Epub 2016 
      Aug 3.