PMID- 27497785
OWN - NLM
STAT- MEDLINE
DCOM- 20170126
LR  - 20181202
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 133
DP  - 2016 Nov
TI  - Identification of interacting proteins with aryl hydrocarbon receptor in scallop 
      Chlamys farreri by yeast two hybrid screening.
PG  - 381-9
LID - S0147-6513(16)30275-5 [pii]
LID - 10.1016/j.ecoenv.2016.07.013 [doi]
AB  - The aryl hydrocarbon receptor (AhR) belongs to the basic-helix-loop helix (bHLH) 
      Per-Arnt-Sim (PAS) family of transcription factors. AhR has been known primarily 
      for its role in the regulation of several drug and xenobiotic metabolizing 
      enzymes, as well as the mediation of the toxicity of certain xenobiotics, 
      including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Although the AhR is 
      well-studied as a mediator of the toxicity of certain xenobiotics in marine 
      bivalves, the normal physiological function remains unknown. In order to explore 
      the function of the AhR, the bait protein expression plasmid pGBKT7-CfAhR and the 
      cDNA library of gill from Chlamys farreri were constructed. By yeast two hybrid 
      system, after multiple screening with the high screening rate medium, rotary 
      verification, sequencing and bioinformatics analysis, the interactions of the 
      CfAhR with receptor for activated protein kinase C 1 (RACK1), thyroid 
      peroxidase-like protein (TPO), Toll-like receptor 4(TLR 4), androglobin-like, 
      store-operated Ca(2+) entry (SocE), ADP/ATP carrier protein, cytochrome b, 
      thioesterase, actin, ferritin subunit 1, poly-ubiquitin, short-chain collagen 
      C4-like and one hypothetical protein in gill cells were identified. This study 
      suggests that the CfAhR played fundamental roles in immune system homeostasis, 
      oxidative stress response, and in grow and development of C. farreri. The 
      elucidation of these protein interactions is of much importance both in 
      understanding the normal physiological function of AhR, and as potential targets 
      for further research on protein function in AhR interactions.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Cai, Yuefeng
AU  - Cai Y
AD  - Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 
      Qingdao 266003, PR China.
FAU - Pan, Luqing
AU  - Pan L
AD  - Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 
      Qingdao 266003, PR China. Electronic address: panlq@ouc.edu.cn.
FAU - Miao, Jingjing
AU  - Miao J
AD  - Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 
      Qingdao 266003, PR China.
FAU - Liu, Tong
AU  - Liu T
AD  - Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 
      Qingdao 266003, PR China.
LA  - eng
PT  - Journal Article
DEP - 20160804
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (DNA, Complementary)
RN  - 0 (Proteins)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Xenobiotics)
SB  - IM
MH  - Animals
MH  - DNA, Complementary
MH  - Gills/metabolism
MH  - Pectinidae/*metabolism
MH  - Proteins/*metabolism
MH  - Receptors, Aryl Hydrocarbon/genetics/*metabolism
MH  - Saccharomyces cerevisiae/metabolism
MH  - Xenobiotics/metabolism
OTO - NOTNLM
OT  - AhR
OT  - Chlamys farreri
OT  - Protein interaction
OT  - Receptor for activated protein kinase C 1 (RACK1)
OT  - Thyroid peroxidase-like protein (TPO)
OT  - Toll-like receptor (TLR)
EDAT- 2016/08/09 06:00
MHDA- 2017/01/27 06:00
CRDT- 2016/08/08 06:00
PHST- 2016/04/25 00:00 [received]
PHST- 2016/07/08 00:00 [revised]
PHST- 2016/07/11 00:00 [accepted]
PHST- 2016/08/08 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2017/01/27 06:00 [medline]
AID - S0147-6513(16)30275-5 [pii]
AID - 10.1016/j.ecoenv.2016.07.013 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2016 Nov;133:381-9. doi: 10.1016/j.ecoenv.2016.07.013. 
      Epub 2016 Aug 4.