PMID- 27498557
OWN - NLM
STAT- MEDLINE
DCOM- 20180620
LR  - 20231213
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Aug 8
TI  - A novel recombinant cell fluorescence biosensor based on toxicity of pathway for 
      rapid and simple evaluation of DON and ZEN.
PG  - 31270
LID - 10.1038/srep31270 [doi]
LID - 31270
AB  - During an exposure, humans and animals are most often exposed to a mixture rather 
      than individual mycotoxins. In this study, a Human Embryonic Kidney 293 cell 
      (HEK-293) fluorescence sensor was developed to detect and evaluate mycotoxins, 
      deoxynivalenol (DON) and zearalenone (ZEN) compounds, produced by Fusarium 
      culmorum that are common food contaminants. TRE-copGFP (green fluorescent 
      protein) and ERE-TagRFP (red fluorescent protein) plasmids were constructed and 
      cotransfected into HEK-293 cells through a highly efficient, lipid-mediated, 
      DNA-transfection procedure. Results show that fluorescence intensity was 
      proportional to DON and ZEN concentrations, ranging from 2 to 40 ng/mL and 10 to 
      100 ng/mL respectively, with a detection limit of 0.75 ng/mL and 3.2 ng/mL 
      respectively. The EC50 of DON and ZEN are 30.13 ng/mL and 76.63 ng/mL 
      respectively. Additionally, ZEN may have a synergistic effect on enhancing AP-1 
      activity of the toxicity pathway of DON. These data indicate the high sensitivity 
      and effectiveness of our biosensor system in the evaluation of the combined 
      toxicity of ZEN, DON and their derivatives. In addition, this approach is 
      suitable for an early warning method for the detection of ZEN and DON family 
      mycotoxins contamination without higher-priced, conventional analytical chemistry 
      methods.
FAU - Ji, Jian
AU  - Ji J
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
FAU - Gu, Wenshu
AU  - Gu W
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
FAU - Sun, Chao
AU  - Sun C
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
FAU - Sun, Jiadi
AU  - Sun J
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
FAU - Jiang, Hui
AU  - Jiang H
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
FAU - Zhang, Yinzhi
AU  - Zhang Y
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
FAU - Sun, Xiulan
AU  - Sun X
AD  - State Key Laboratory of Food Science and Technology, School of Food Science of 
      Jiangnan University, School of Food Science Synergetic Innovation Center of Food 
      Safety and Nutrition, Wuxi, Jiangsu, 214122, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160808
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Lipids)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Mycotoxins)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Trichothecenes)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 5W827M159J (Zearalenone)
RN  - 9007-49-2 (DNA)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - *Biosensing Techniques
MH  - Cell Separation
MH  - DNA/chemistry
MH  - Flow Cytometry
MH  - Food Contamination/*analysis
MH  - Fusarium/chemistry
MH  - Green Fluorescent Proteins/chemistry
MH  - HEK293 Cells
MH  - Humans
MH  - Lipids/chemistry
MH  - Luminescent Proteins/chemistry
MH  - Mycotoxins/*analysis
MH  - Reactive Oxygen Species/chemistry
MH  - Recombinant Proteins/*chemistry
MH  - Transfection
MH  - Trichothecenes/*analysis
MH  - Zearalenone/*analysis
MH  - Red Fluorescent Protein
PMC - PMC4976381
EDAT- 2016/08/09 06:00
MHDA- 2018/06/21 06:00
PMCR- 2016/08/08
CRDT- 2016/08/09 06:00
PHST- 2016/01/21 00:00 [received]
PHST- 2016/07/15 00:00 [accepted]
PHST- 2016/08/09 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2016/08/08 00:00 [pmc-release]
AID - srep31270 [pii]
AID - 10.1038/srep31270 [doi]
PST - epublish
SO  - Sci Rep. 2016 Aug 8;6:31270. doi: 10.1038/srep31270.