PMID- 27498801
OWN - NLM
STAT- MEDLINE
DCOM- 20170127
LR  - 20191210
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 33
IP  - 4
DP  - 2016 Aug
TI  - A Stenohaline Medaka, Oryzias woworae, Increases Expression of Gill Na(+), 
      K(+)-ATPase and Na(+), K(+), 2Cl(-) Cotransporter 1 to Tolerate Osmotic Stress.
PG  - 414-25
LID - 10.2108/zs150157 [doi]
AB  - The present study aimed to evaluate the osmoregulatory mechanism of Daisy's 
      medaka, O. woworae,as well as demonstrate the major factors affecting the 
      hypo-osmoregulatory characteristics of euryhaline and stenohaline medaka. The 
      medaka phylogenetic tree indicates that Daisy's medaka belongs to the celebensis 
      species group. The salinity tolerance of Daisy's medaka was assessed. Our 
      findings revealed that 20 per thousand (hypertonic) saltwater (SW) was lethal to Daisy's 
      medaka. However, 62.5% of individuals survived 10 per thousand (isotonic) SW with 
      pre-acclimation to 5 per thousand SW for one week. This transfer regime, "Experimental (Exp.) 
      10 per thousand SW", was used in the following experiments. After 10 per thousand SW-transfer, the plasma 
      osmolality of Daisy's medaka significantly increased. The protein abundance and 
      distribution of branchial Na(+), K(+)-ATPase (NKA) and Na(+), K(+), 2Cl(-) 
      cotransporter 1 (NKCC1) were also examined after transfer to 10 per thousand SW for one week. 
      Gill NKA activity increased significantly after transfer to 10 per thousand SW. Meanwhile, 
      elevation of gill NKA alphaalpha-subunit protein-abundance was found in the 10 per thousand 
      SW-acclimated fish. In gill cross-sections, more and larger NKA-immunoreactive 
      (NKA-IR) cells were observed in the Exp. 10 per thousand SW medaka. The relative abundance of 
      branchial NKCC1 protein increased significantly after transfer to 10 per thousand SW. NKCC1 
      was distributed in the basolateral membrane of NKA-IR cells of the Exp. 10 per thousand SW 
      group. Furthermore, a higher abundance of NKCC1 protein was found in the gill 
      homogenates of the euryhaline medaka, O. dancena, than in that of the stenohaline 
      medaka, O. woworae.
FAU - Juo, Jiun-Jang
AU  - Juo JJ
AD  - 1 Department of Life Sciences, National Chung Hsing University, Taichung 402, 
      Taiwan.
AD  - dagger JJJ, CKK, and WKY contributed equally to this paper.
FAU - Kang, Chao-Kai
AU  - Kang CK
AD  - 2 Tainan Hydraulics Laboratory, National Cheng Kung University, Tainan 709, 
      Taiwan.
AD  - dagger JJJ, CKK, and WKY contributed equally to this paper.
FAU - Yang, Wen-Kai
AU  - Yang WK
AD  - 1 Department of Life Sciences, National Chung Hsing University, Taichung 402, 
      Taiwan.
AD  - dagger JJJ, CKK, and WKY contributed equally to this paper.
FAU - Yang, Shu-Yuan
AU  - Yang SY
AD  - 1 Department of Life Sciences, National Chung Hsing University, Taichung 402, 
      Taiwan.
FAU - Lee, Tsung-Han
AU  - Lee TH
AD  - 1 Department of Life Sciences, National Chung Hsing University, Taichung 402, 
      Taiwan.
AD  - 3 Department of Biological Science and Technology, China Medical 
      University,Taichung 404, Taiwan.
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 059QF0KO0R (Water)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Adaptation, Physiological/genetics/physiology
MH  - Animals
MH  - Gene Expression Regulation, Enzymologic
MH  - Gills/*enzymology
MH  - Oryzias/classification/*metabolism
MH  - Osmotic Pressure/*physiology
MH  - Phylogeny
MH  - Salinity
MH  - Sodium-Potassium-Chloride Symporters/genetics/*metabolism
MH  - Sodium-Potassium-Exchanging ATPase/genetics/*metabolism
MH  - Species Specificity
MH  - Water/chemistry
OTO - NOTNLM
OT  - 2Cl- cotransporter 1
OT  - K+
OT  - K+-ATPase
OT  - Na+
OT  - Oryzias woworae
OT  - gill
OT  - osmotic stress
OT  - stenohaline
EDAT- 2016/08/09 06:00
MHDA- 2017/01/28 06:00
CRDT- 2016/08/09 06:00
PHST- 2016/08/09 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2017/01/28 06:00 [medline]
AID - 10.2108/zs150157 [doi]
PST - ppublish
SO  - Zoolog Sci. 2016 Aug;33(4):414-25. doi: 10.2108/zs150157.