PMID- 27499068
OWN - NLM
STAT- MEDLINE
DCOM- 20180620
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 6
DP  - 2016 Aug 8
TI  - Increased EZH2 and decreased osteoblastogenesis during local irradiation-induced 
      bone loss in rats.
PG  - 31318
LID - 10.1038/srep31318 [doi]
LID - 31318
AB  - Radiation therapy is commonly used to treat cancer patients but exhibits adverse 
      effects, including insufficiency fractures and bone loss. Epigenetic regulation 
      plays an important role in osteogenic differentiation of bone marrow mesenchymal 
      stem cells (BMSCs). Here, we reported local bone changes after single-dose 
      exposure to (137)CS irradiation in rats. Femur bone mineral density (BMD) and 
      trabecular bone volume in the tibia were significantly decreased at 12 weeks 
      after irradiation. Micro-CT results showed that tBMD, Tb.h and Tb.N were also 
      significantly reduced at 12 weeks after irradiation exposure. ALP-positive 
      OB.S/BS was decreased by 42.3% at 2 weeks after irradiation and was decreased by 
      50.8% at 12 weeks after exposure. In contrast to the decreased expression of 
      Runx2 and BMP2, we found EZH2 expression was significantly increased at 2 weeks 
      after single-dose (137)CS irradiation in BMSCs. Together, our results 
      demonstrated that single-dose (137)CS irradiation induces BMD loss and the 
      deterioration of bone microarchitecture in the rat skeleton. Furthermore, EZH2 
      expression increased and osteoblastogenesis decreased after irradiation. The 
      underlying mechanisms warrant further investigation.
FAU - Guo, Changjun
AU  - Guo C
AD  - Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint 
      Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of 
      Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University 
      School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
FAU - Li, Changwei
AU  - Li C
AD  - Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint 
      Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of 
      Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University 
      School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
FAU - Yang, Kai
AU  - Yang K
AD  - Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint 
      Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of 
      Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University 
      School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
FAU - Kang, Hui
AU  - Kang H
AD  - Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint 
      Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of 
      Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University 
      School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
FAU - Xu, Xiaoya
AU  - Xu X
AD  - Department of Bone Metabolism, Institute of Radiation Medicine, Fudan University, 
      Shanghai 200032, China. Address: No. 2094, Xietu Road, Shanghai 200032 China.
FAU - Xu, Xiangyang
AU  - Xu X
AD  - Department of Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University School 
      of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
FAU - Deng, Lianfu
AU  - Deng L
AD  - Shanghai Key Laboratory for the Prevention and Treatment of Bone and Joint 
      Diseases with Integrated Chinese-Western Medicine, Shanghai Institute of 
      Traumatology and Orthopedics, Rui Jin Hospital, Shanghai Jiao Tong University 
      School of Medicine. Address: No. 197, Rui Jin Er Road, Shanghai 200025 China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160808
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - EC 2.1.1.43 (EZH2 protein, rat)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Bone Demineralization, Pathologic/etiology/*metabolism
MH  - Bone Density
MH  - Bone Marrow Cells/cytology
MH  - Cell Differentiation
MH  - Enhancer of Zeste Homolog 2 Protein/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epigenesis, Genetic
MH  - Femur/diagnostic imaging/radiation effects
MH  - Male
MH  - Neoplasms/metabolism
MH  - Osteoblasts/*metabolism
MH  - Osteogenesis
MH  - Rats
MH  - Tibia/diagnostic imaging/radiation effects
MH  - X-Ray Microtomography
PMC - PMC4976370
EDAT- 2016/08/09 06:00
MHDA- 2018/06/21 06:00
PMCR- 2016/08/08
CRDT- 2016/08/09 06:00
PHST- 2016/03/03 00:00 [received]
PHST- 2016/07/05 00:00 [accepted]
PHST- 2016/08/09 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2016/08/08 00:00 [pmc-release]
AID - srep31318 [pii]
AID - 10.1038/srep31318 [doi]
PST - epublish
SO  - Sci Rep. 2016 Aug 8;6:31318. doi: 10.1038/srep31318.