PMID- 27501106
OWN - NLM
STAT- MEDLINE
DCOM- 20180321
LR  - 20190216
IS  - 1536-5948 (Electronic)
IS  - 1076-2752 (Print)
IS  - 1076-2752 (Linking)
VI  - 58
IP  - 8 Suppl 1
DP  - 2016 Aug
TI  - Detection of Serum microRNAs From Department of Defense Serum Repository: 
      Correlation With Cotinine, Cytokine, and Polycyclic Aromatic Hydrocarbon Levels.
PG  - S62-71
LID - 10.1097/JOM.0000000000000742 [doi]
AB  - OBJECTIVE: The aim of this study was to investigate whether serum samples from 
      the Department of Defense Serum Repository (DoDSR) are of sufficient quality to 
      detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic 
      aromatic hydrocarbons (PAHs). METHODS: MiRNAs were isolated and quantified by 
      polymerase chain reaction (PCR) array. Cytokines and chemokines related to 
      inflammation were measured using multiplex immunoassays. Cotinine and IgE were 
      detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid 
      Chromatography/Mass Spectroscopy. RESULTS: We detected miRNAs, cytokines, IgE, 
      and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine 
      suggesting tobacco exposure. Significant associations between serum cotinine, 
      cytokine, IgE, PAHs, and miRNA were discovered. CONCLUSION: We successfully 
      quantified over 200 potential biomarkers of occupational exposure from DoDSR 
      samples. The stored serum samples were not affected by hemolysis and represent a 
      powerful tool for biomarker discovery and analysis in retrospective studies.
FAU - Woeller, Collynn F
AU  - Woeller CF
AD  - Department of Environmental Medicine (Dr Woeller, Mr Pollock, Ms Croasdell, Ms 
      Kim, Dr Utell, Dr Phipps), Department of Medicine (Drs Thatcher, Utell, and 
      Phipps), Microbiology and Immunology, University of Rochester Medical Center (Mr 
      Van Twisk, Drs Thakar and Phipps); Center for Air Resources Engineering and 
      Science, Clarkson University, Potsdam, New York (Drs Hopke and Xia); and 
      Professor, Department of Preventive Medicine and Biostatistics, Uniformed 
      Services University, Bethesda, MD 20814 (Dr Mallon).
FAU - Thatcher, Thomas H
AU  - Thatcher TH
FAU - Van Twisk, Daniel
AU  - Van Twisk D
FAU - Pollock, Stephen J
AU  - Pollock SJ
FAU - Croasdell, Amanda
AU  - Croasdell A
FAU - Kim, Nina
AU  - Kim N
FAU - Hopke, Philip K
AU  - Hopke PK
FAU - Xia, Xiaoyan
AU  - Xia X
FAU - Thakar, Juilee
AU  - Thakar J
FAU - Mallon, Col Timothy M
AU  - Mallon CT
FAU - Utell, Mark J
AU  - Utell MJ
FAU - Phipps, Richard P
AU  - Phipps RP
LA  - eng
GR  - P30 ES001247/ES/NIEHS NIH HHS/United States
GR  - T32 ES007026/ES/NIEHS NIH HHS/United States
GR  - T90 DE021985/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - J Occup Environ Med
JT  - Journal of occupational and environmental medicine
JID - 9504688
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - K5161X06LL (Cotinine)
SB  - IM
MH  - Biomarkers/blood
MH  - Blood Banks
MH  - Cotinine/*blood
MH  - Cytokines/*blood
MH  - Environmental Exposure/analysis
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - MicroRNAs/*blood
MH  - *Military Personnel
MH  - Occupational Exposure/analysis
MH  - Polycyclic Aromatic Hydrocarbons/*blood
MH  - Retrospective Studies
PMC - PMC5661968
MID - NIHMS910864
COIS- There are no conflicts of interest.
EDAT- 2016/08/09 06:00
MHDA- 2018/03/22 06:00
PMCR- 2017/10/30
CRDT- 2016/08/09 06:00
PHST- 2016/08/09 06:00 [entrez]
PHST- 2016/08/09 06:00 [pubmed]
PHST- 2018/03/22 06:00 [medline]
PHST- 2017/10/30 00:00 [pmc-release]
AID - 00043764-201608001-00009 [pii]
AID - 10.1097/JOM.0000000000000742 [doi]
PST - ppublish
SO  - J Occup Environ Med. 2016 Aug;58(8 Suppl 1):S62-71. doi: 
      10.1097/JOM.0000000000000742.