PMID- 27501706
OWN - NLM
STAT- MEDLINE
DCOM- 20170323
LR  - 20181202
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 60
IP  - 4
DP  - 2016 Dec
TI  - Effect of Laminin on Neurotrophic Factors Expression in Schwann-Like Cells 
      Induced from Human Adipose-Derived Stem Cells In Vitro.
PG  - 465-473
AB  - The Schwann-like cells can be considered as promising in stem cell therapies, at 
      least in experimental models. Human adipose-derived stem cells (ADSCs) are 
      induced into Schwann-like cells (SC-like cells) and are cultured on either a 
      plastic surface or laminin-coated plates. The findings here reveal that laminin 
      is a critical component in extracellular matrix (ECM) of SC-like cells at in 
      vitro. The survival rate of SC-like cells on a laminin matrix are measured 
      through MTT assay and it is found that this rate is significantly higher than 
      that of the cells grown on a plastic surface (P < 0.05). Schwann cell markers and 
      the myelinogenic ability of SC-like cells at the presence versus absence of 
      laminin are assessed through immunocytochemistry. The analysis of GFAP/S100beta and 
      S100beta/MBP markers indicate that laminin can increase the differentiated rate and 
      myelinogenic potential of SC-like cells. The expression levels of SCs markers, 
      myelin basic proteins (MBP), and neurotrophic factors in two conditions are 
      analyzed by real-time reverse transcription polymerase chain reaction (RT-PCR). 
      The findings here demonstrated that gene expression of SCs markers, MBP, and 
      brain-derived neurotrophic factors (BDNF) increase significantly on laminin 
      compared to plastic surface (P < 0.01). In contrast, the nerve growth factor 
      (NGF) expression is downregulated significantly on laminin-coated plates 
      (P < 0.05). The obtained data suggest that production of neurotrophic factors in 
      SC-like cell in presence of laminin can induce appropriate microenvironment for 
      nerve repair in neurodegenerative diseases.
FAU - Zarinfard, Giti
AU  - Zarinfard G
AD  - Department of Anatomical Sciences, School of Medicine, Isfahan University of 
      Medical Sciences, Isfahan, 81744-176, Iran.
AD  - Department of Anatomical Sciences, School of Veterinary Medicine, Shiraz 
      University, Shiraz, 71345-1731, Iran.
FAU - Tadjalli, Mina
AU  - Tadjalli M
AD  - Department of Anatomical Sciences, School of Veterinary Medicine, Shiraz 
      University, Shiraz, 71345-1731, Iran.
FAU - Razavi, Shahnaz
AU  - Razavi S
AD  - Department of Anatomical Sciences, School of Medicine, Isfahan University of 
      Medical Sciences, Isfahan, 81744-176, Iran. razavi@med.mui.ac.ir.
FAU - Kazemi, Mohammad
AU  - Kazemi M
AD  - Department of Molecular Biology and Genetic, School of Medicine, Isfahan 
      University of Medical Sciences, Isfahan, Iran.
LA  - eng
PT  - Journal Article
DEP - 20160809
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glial Fibrillary Acidic Protein)
RN  - 0 (Laminin)
RN  - 0 (Myelin Basic Protein)
RN  - 0 (S100 Proteins)
RN  - 9061-61-4 (Nerve Growth Factor)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Adult
MH  - Brain-Derived Neurotrophic Factor/genetics/metabolism
MH  - Cell Differentiation/*drug effects
MH  - Cells, Cultured
MH  - Female
MH  - Glial Fibrillary Acidic Protein/genetics/metabolism
MH  - Humans
MH  - Laminin/*pharmacology
MH  - Mesenchymal Stem Cells/cytology/*drug effects/metabolism
MH  - Middle Aged
MH  - Myelin Basic Protein/genetics/metabolism
MH  - Nerve Growth Factor/genetics/metabolism
MH  - S100 Proteins/genetics/metabolism
MH  - Schwann Cells/cytology/*drug effects/metabolism
OTO - NOTNLM
OT  - Adipose-derived stem cells
OT  - Laminin
OT  - Myelin
OT  - Neurotrophic factors
OT  - Schwann cell
EDAT- 2016/08/10 06:00
MHDA- 2017/03/24 06:00
CRDT- 2016/08/10 06:00
PHST- 2016/06/15 00:00 [received]
PHST- 2016/07/28 00:00 [accepted]
PHST- 2016/08/10 06:00 [pubmed]
PHST- 2017/03/24 06:00 [medline]
PHST- 2016/08/10 06:00 [entrez]
AID - 10.1007/s12031-016-0808-6 [pii]
AID - 10.1007/s12031-016-0808-6 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2016 Dec;60(4):465-473. doi: 10.1007/s12031-016-0808-6. Epub 2016 
      Aug 9.