PMID- 27502455
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20181202
IS  - 1614-7499 (Electronic)
IS  - 0944-1344 (Linking)
VI  - 23
IP  - 21
DP  - 2016 Nov
TI  - A self-sufficient system for removal of synthetic dye by coupling of 
      spore-displayed triphenylmethane reductase and glucose 1-dehydrogenase.
PG  - 21319-21326
AB  - Biodegradation of triphenylmethane dyes by microorganisms is hampered by the 
      transport barrier imposed by cell membranes. On the other hand, cell-free systems 
      using enzyme-based biodegradation strategy are costly. Therefore, an efficient 
      and inexpensive approach circumventing these problems is highly desirable. Here, 
      we constructed a self-sufficient system for synthetic dye removal by coupling of 
      spore surface-displayed triphenylmethane reductase (TMR) and glucose 
      1-dehydrogenase (GDH) for the first time. Display of both TMR and GDH 
      significantly enhanced their stability under conditions of extreme pH and 
      temperature. These engineered spores also exhibited more robust long-term 
      stability than their purified counterparts. Furthermore, we observed that a high 
      ratio of spore-displayed GDH is necessary for high dye degradation efficiency. 
      These results indicate that this continuous dye removal system with cofactor 
      regeneration offers a promising solution for dye biodegradation applications.
FAU - Gao, Fen
AU  - Gao F
AD  - College of Life Science, Zhejiang University, Hangzhou, 310058, China.
FAU - Ding, Haitao
AU  - Ding H
AD  - Polar Research Institute of China, Shanghai, 200136, China.
FAU - Xu, Xiaohong
AU  - Xu X
AD  - College of Life Science, Zhejiang University, Hangzhou, 310058, China.
FAU - Zhao, Yuhua
AU  - Zhao Y
AD  - College of Life Science, Zhejiang University, Hangzhou, 310058, China. 
      yhzhao225@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20160808
PL  - Germany
TA  - Environ Sci Pollut Res Int
JT  - Environmental science and pollution research international
JID - 9441769
RN  - 0 (Coloring Agents)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Trityl Compounds)
RN  - 8O4UTW9E17 (triphenylmethane)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.1.1.47 (Glucose 1-Dehydrogenase)
SB  - IM
MH  - Bacillus subtilis/*metabolism
MH  - Biodegradation, Environmental
MH  - Coloring Agents/*isolation & purification/*metabolism
MH  - Environmental Pollutants/isolation & purification/metabolism
MH  - Glucose 1-Dehydrogenase/metabolism
MH  - Oxidoreductases/*isolation & purification/*metabolism
MH  - Spores, Bacterial/*metabolism
MH  - Temperature
MH  - Trityl Compounds/*metabolism
OTO - NOTNLM
OT  - Bacillus subtilis
OT  - Biodegradation
OT  - Cofactor regeneration
OT  - Glucose 1-dehydrogenase (GDH)
OT  - Spore surface display (ssd)
OT  - Triphenylmethane dye
OT  - Triphenylmethane reductase (TMR)
EDAT- 2016/08/10 06:00
MHDA- 2017/04/04 06:00
CRDT- 2016/08/10 06:00
PHST- 2016/02/20 00:00 [received]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/08/10 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
PHST- 2016/08/10 06:00 [entrez]
AID - 10.1007/s11356-016-7330-9 [pii]
AID - 10.1007/s11356-016-7330-9 [doi]
PST - ppublish
SO  - Environ Sci Pollut Res Int. 2016 Nov;23(21):21319-21326. doi: 
      10.1007/s11356-016-7330-9. Epub 2016 Aug 8.