PMID- 27502588
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20201024
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 347
IP  - 1
DP  - 2016 Sep 10
TI  - Inhibition of hydrogen sulfide on the proliferation of vascular smooth muscle 
      cells involved in the modulation of calcium sensing receptor in high 
      homocysteine.
PG  - 184-191
LID - S0014-4827(16)30234-8 [pii]
LID - 10.1016/j.yexcr.2016.08.004 [doi]
AB  - Hyperhomocysteinemia induces the proliferation of vascular smooth muscle cells 
      (VSMCs). Hydrogen sulfide (H2S) inhibits the phenotype switch of VSMCs and 
      calcium-sensing receptor (CaSR) regulated the production of endogenous H2S. 
      However, whether CaSR inhibits the proliferation of VSMCs by regulating the 
      endogenous cystathionine-gamma-lyase (CSE, a major enzyme that produces H2S) 
      pathway in high homocysteine (HHcy) has not been previously investigated. The 
      intracellular calcium concentration, the concentration of H2S, the cell 
      viability, the proliferation and the expression of proteins of cultured VSMCs 
      from rat thoracic aortas were measured, respectively. The results showed that the 
      [Ca(2+)]i and the expression of p-CaMK and CSE increased upon treatment with CaSR 
      agonist. In HHcy, the H2S concentration decrease, the proliferation and migration 
      rate increased, the expression of Cyclin D1, PCNA, Osteopontin and p-Erk1/2 
      increased while the alpha-SM actin, P21(Cip/WAK-1) and Calponin decreased. The CaSR 
      agonist or exogenous H2S significantly reversed the changes of VSMCs caused by 
      HHcy. In conclusion, our results demonstrated that CaSR regulate the endogenous 
      CSE/H2S is related to the PLC-IP3 receptor and CaM signal pathways which inhibit 
      the proliferation of VSMCs, and the latter is involved in the Erk1/2 dependent 
      signal pathway in high homocysteine.
CI  - Copyright (c) 2016 Elsevier Inc. All rights reserved.
FAU - Wang, Yuwen
AU  - Wang Y
AD  - Department of Clinical Laboratory, The second Affiliated Hospital of Harbin 
      Medical University, Harbin 150081, China.
FAU - Wang, Xiyao
AU  - Wang X
AD  - Department of Clinical Laboratory, The second Affiliated Hospital of Harbin 
      Medical University, Harbin 150081, China.
FAU - Liang, Xiaohui
AU  - Liang X
AD  - Department of Radiology, Central Hospital of the Red Cross, Harbin 150080, China.
FAU - Wu, Jichao
AU  - Wu J
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
FAU - Dong, Shiyun
AU  - Dong S
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
FAU - Li, Hongzhu
AU  - Li H
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
FAU - Jin, Meili
AU  - Jin M
AD  - Department of Clinical Laboratory, The second Affiliated Hospital of Harbin 
      Medical University, Harbin 150081, China.
FAU - Sun, Dianjun
AU  - Sun D
AD  - Center for Endemic Disease Control, Chinese Center for Disease Control and 
      Prevention, Harbin Medical University, Harbin 150086, China.
FAU - Zhang, Weihua
AU  - Zhang W
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150081, China.
FAU - Zhong, Xin
AU  - Zhong X
AD  - Department of Pathophysiology, Harbin Medical University, Harbin 150081, China. 
      Electronic address: xzhong1111@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20160805
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 ((R)-2-(1-(1-naphthyl)ethyl-aminom-ethyl)indole)
RN  - 0 (Calmodulin)
RN  - 0 (Indoles)
RN  - 0 (Naphthalenes)
RN  - 0 (Receptors, Calcium-Sensing)
RN  - 0LVT1QZ0BA (Homocysteine)
RN  - 85166-31-0 (Inositol 1,4,5-Trisphosphate)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - G34N38R2N1 (Bromodeoxyuridine)
RN  - SY7Q814VUP (Calcium)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Adolescent
MH  - Animals
MH  - Bromodeoxyuridine/metabolism
MH  - Calcium/metabolism
MH  - Calmodulin/metabolism
MH  - Cell Movement/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cystathionine gamma-Lyase/metabolism
MH  - Homocysteine/*pharmacology
MH  - Humans
MH  - Hydrogen Sulfide/*pharmacology
MH  - Indoles/pharmacology
MH  - Inositol 1,4,5-Trisphosphate/metabolism
MH  - MAP Kinase Signaling System/drug effects
MH  - Muscle, Smooth, Vascular/*cytology
MH  - Myocytes, Smooth Muscle/drug effects/*metabolism
MH  - Naphthalenes/pharmacology
MH  - Phenotype
MH  - Rats
MH  - Receptors, Calcium-Sensing/*metabolism
MH  - Signal Transduction/drug effects
MH  - Type C Phospholipases/metabolism
OTO - NOTNLM
OT  - Calcium sensing receptor
OT  - High homocysteine
OT  - Hydrogen sulfide
OT  - Smooth muscle cells
EDAT- 2016/08/10 06:00
MHDA- 2017/05/16 06:00
CRDT- 2016/08/10 06:00
PHST- 2016/06/04 00:00 [received]
PHST- 2016/08/01 00:00 [revised]
PHST- 2016/08/04 00:00 [accepted]
PHST- 2016/08/10 06:00 [entrez]
PHST- 2016/08/10 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
AID - S0014-4827(16)30234-8 [pii]
AID - 10.1016/j.yexcr.2016.08.004 [doi]
PST - ppublish
SO  - Exp Cell Res. 2016 Sep 10;347(1):184-191. doi: 10.1016/j.yexcr.2016.08.004. Epub 
      2016 Aug 5.