PMID- 27503149
OWN - NLM
STAT- MEDLINE
DCOM- 20180118
LR  - 20181113
IS  - 1872-7549 (Electronic)
IS  - 0166-4328 (Print)
IS  - 0166-4328 (Linking)
VI  - 314
DP  - 2016 Nov 1
TI  - A sex difference in oxidative stress and behavioral suppression induced by 
      ethanol withdrawal in rats.
PG  - 199-214
LID - S0166-4328(16)30492-2 [pii]
LID - 10.1016/j.bbr.2016.07.054 [doi]
AB  - Ethanol withdrawal (EW) is referred to the abrupt termination of long-term heavy 
      drinking, and provokes oxidative brain damage. Here, we investigated whether the 
      cerebellum and hippocampus of female rats are less affected by prooxidant EW than 
      male rats due to the antioxidant effect of 17beta-estradiol (E2). Female and male 
      rats received a four-week ethanol diet and three-week withdrawal per cycle for 
      two cycles. Some female rats were ovariectomized with E2 or antioxidant (Vitamin 
      E+Co-Q10) treatment. Measurements were cerebellum (Rotarod) and hippocampus 
      (water-maze)-related behaviors, oxidative markers (O2(-), malondialdehyde, 
      protein carbonyls), mitochondrial membrane swelling, and a key mitochondrial 
      enzyme, cytochrome c oxidase (CcO). Separately, HT22 (hippocampal) cells were 
      subjected to ethanol-exposure and withdrawal for two cycles to assess the effect 
      of a CcO inhibitor on E2's protection for mitochondrial respiration and cell 
      viability. Ethanol-withdrawn female rats showed a smaller increase in oxidative 
      markers in cerebellum and hippocampus than male rats, and E2 treatment decreased 
      the oxidative markers. Compared to male counterparts, ethanol-withdrawn female 
      rats showed better Rotarod but poorer water-maze performance, accompanied by more 
      severe mitochondrial membrane swelling and CcO suppression in hippocampus. E2 or 
      antioxidant treatment improved Rotarod but not water-maze performance. In the 
      presence of a CcO inhibitor, E2 treatment failed to protect mitochondrial 
      respiration and cell viability from EW. These data suggest that antioxidant E2 
      contributes to smaller oxidative stress in ethanol-withdrawn female than male 
      rats. They also suggest that EW-induced severe mitochondrial damage in 
      hippocampus may blunt E2's antioxidant protection for hippocampus-related 
      behavior.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Jung, Marianna E
AU  - Jung ME
AD  - Department of Pharmacology and Neuroscience, University of North Texas Health 
      Science Center at Fort Worth, 3500 Camp Bowie Blvd., Fort Worth, TX 76107-2699, 
      USA. Electronic address: Marianna.Jung@unthsc.edu.
FAU - Metzger, Daniel B
AU  - Metzger DB
AD  - Department of Pharmacology and Neuroscience, University of North Texas Health 
      Science Center at Fort Worth, 3500 Camp Bowie Blvd., Fort Worth, TX 76107-2699, 
      USA.
LA  - eng
GR  - R01 AA015982/AA/NIAAA NIH HHS/United States
GR  - R21 AA018747/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20160805
PL  - Netherlands
TA  - Behav Brain Res
JT  - Behavioural brain research
JID - 8004872
RN  - 0 (Antioxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 3K9958V90M (Ethanol)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Estradiol/pharmacology
MH  - Ethanol/*pharmacology
MH  - Female
MH  - Male
MH  - Mice
MH  - Mitochondria/drug effects/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - *Sex Characteristics
MH  - Substance Withdrawal Syndrome/*metabolism
PMC - PMC5002225
MID - NIHMS809951
OTO - NOTNLM
OT  - 17beta-Estradiol
OT  - Antioxidant
OT  - Cerebellum
OT  - Cytochrome c oxidase
OT  - Ethanol withdrawal
OT  - Hippocampus
EDAT- 2016/08/10 06:00
MHDA- 2018/01/19 06:00
PMCR- 2017/11/01
CRDT- 2016/08/10 06:00
PHST- 2016/05/19 00:00 [received]
PHST- 2016/07/25 00:00 [revised]
PHST- 2016/07/30 00:00 [accepted]
PHST- 2016/08/10 06:00 [entrez]
PHST- 2016/08/10 06:00 [pubmed]
PHST- 2018/01/19 06:00 [medline]
PHST- 2017/11/01 00:00 [pmc-release]
AID - S0166-4328(16)30492-2 [pii]
AID - 10.1016/j.bbr.2016.07.054 [doi]
PST - ppublish
SO  - Behav Brain Res. 2016 Nov 1;314:199-214. doi: 10.1016/j.bbr.2016.07.054. Epub 
      2016 Aug 5.