PMID- 27510626
OWN - NLM
STAT- MEDLINE
DCOM- 20170522
LR  - 20210109
IS  - 1432-1084 (Electronic)
IS  - 0938-7994 (Linking)
VI  - 27
IP  - 4
DP  - 2017 Apr
TI  - DNA damage in lymphocytes induced by cardiac CT and comparison with physical 
      exposure parameters.
PG  - 1660-1666
LID - 10.1007/s00330-016-4519-8 [doi]
AB  - OBJECTIVES: To investigate whether physical exposure parameters such as the dose 
      index (CTDI), dose length product (DLP), and size-specific dose estimate (SSDE) 
      are predictive of DNA damage. METHODS: In vitro, we scanned a phantom containing 
      blood samples from five volunteers at CTDI 50, 100, and 150 mGy. One sample was 
      not scanned. We also scanned samples in three different-size phantoms at CTDI 100 
      mGy. In vivo, we enrolled 45 patients and obtained blood samples before and after 
      cardiac CT. The gamma-H2AX foci were counted. RESULTS: In vitro, in the control and 
      at CTDI 50, 100, and 150 mGy, the number of gamma-H2AX was 0.94 +/- 0.24 (standard 
      error, SE), 1.28 +/- 0.30, 1.91 +/- 0.47, and 2.16 +/- 0.20. At SSDE 180, 156, and 135 
      mGy, it was 2.41 +/- 0.20, 1.91 +/- 0.47, and 1.42 +/- 0.20 foci/cell. The gamma-H2AX foci 
      were positively correlated with the radiation dose and negatively correlated with 
      the body size. In vivo, the gamma-H2AX foci were significantly increased after CT 
      (from 1.21 +/- 0.19 to 1.92 +/- 0.22 foci/cell) and correlated with CTDI, DLP, and 
      SSDE. CONCLUSIONS: DNA damage was induced by cardiac CT. There was a correlation 
      between the physical exposure parameters and gamma-H2AX. KEY POINTS: * DNA damage was 
      induced by radiation exposure from cardiac CT. * The gamma-H2AX foci number was 
      correlated with the CT radiation dose. * Physical exposure parameters reflect the 
      DNA damage by CT radiation exposure.
FAU - Fukumoto, Wataru
AU  - Fukumoto W
AUID- ORCID: 0000-0001-7645-8446
AD  - Department of Diagnostic Radiology, Institute of Biomedical Health Sciences, 
      Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima, 734-8551, Japan. 
      wfukumoto@hiroshima-u.ac.jp.
FAU - Ishida, Mari
AU  - Ishida M
AD  - Department of Cardiovascular Physiology and Medicine, Institute of Biomedical and 
      Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Sakai, Chiemi
AU  - Sakai C
AD  - Department of Cardiovascular Physiology and Medicine, Institute of Biomedical and 
      Health Sciences, Hiroshima University, Hiroshima, Japan.
FAU - Tashiro, Satoshi
AU  - Tashiro S
AD  - Department of Cellular Biology, Research Institute for Radiation Biology and 
      Medicine, Hiroshima University, Hiroshima, Japan.
FAU - Ishida, Takafumi
AU  - Ishida T
AD  - Institute of Clinical Research West Medical Center, Hiroshima, Japan.
FAU - Nakano, Yukiko
AU  - Nakano Y
AD  - Department of Cardiovascular Medicine, Hiroshima University Hospital, Hiroshima, 
      Japan.
FAU - Tatsugami, Fuminari
AU  - Tatsugami F
AD  - Department of Diagnostic Radiology, Institute of Biomedical Health Sciences, 
      Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima, 734-8551, Japan.
FAU - Awai, Kazuo
AU  - Awai K
AD  - Department of Diagnostic Radiology, Institute of Biomedical Health Sciences, 
      Hiroshima University, 1-2-3 Kasumi, Minamiku, Hiroshima, 734-8551, Japan.
LA  - eng
PT  - Journal Article
DEP - 20160810
PL  - Germany
TA  - Eur Radiol
JT  - European radiology
JID - 9114774
RN  - 0 (H2AX protein, human)
RN  - 0 (Histones)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Arrhythmias, Cardiac/diagnostic imaging
MH  - Body Size
MH  - *DNA Damage
MH  - Female
MH  - Histones/analysis/drug effects
MH  - Humans
MH  - Lymphocytes/*radiation effects
MH  - Male
MH  - Middle Aged
MH  - Phantoms, Imaging
MH  - Radiation Dosage
MH  - Radiation Exposure
MH  - Tomography, X-Ray Computed/*methods
OTO - NOTNLM
OT  - CT dose index
OT  - Computed tomography
OT  - DNA
OT  - Size-specific dose estimates
OT  - gamma-H2AX
EDAT- 2016/08/12 06:00
MHDA- 2017/05/23 06:00
CRDT- 2016/08/12 06:00
PHST- 2016/02/16 00:00 [received]
PHST- 2016/07/19 00:00 [accepted]
PHST- 2016/07/13 00:00 [revised]
PHST- 2016/08/12 06:00 [pubmed]
PHST- 2017/05/23 06:00 [medline]
PHST- 2016/08/12 06:00 [entrez]
AID - 10.1007/s00330-016-4519-8 [pii]
AID - 10.1007/s00330-016-4519-8 [doi]
PST - ppublish
SO  - Eur Radiol. 2017 Apr;27(4):1660-1666. doi: 10.1007/s00330-016-4519-8. Epub 2016 
      Aug 10.