PMID- 27511166
OWN - NLM
STAT- MEDLINE
DCOM- 20171011
LR  - 20171108
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1429
DP  - 2016
TI  - Localization of Low-Copy DNA Sequences on Mitotic Chromosomes by FISH.
PG  - 49-64
LID - 10.1007/978-1-4939-3622-9_5 [doi]
AB  - Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA 
      sequences on mitotic and meiotic chromosomes and interphase nuclei. It was 
      developed in early 1980s and since then it has contributed to numerous studies 
      and important discoveries. Over the decades, the protocol was modified for ease 
      of use, allowing for localizing multiple probes simultaneously and increasing its 
      sensitivity and specificity. Despite the continuous improvements, the ability to 
      detect short single-copy sequences of only a few kilobases or less, such as 
      genes, remains limited. Here, we provide a detailed protocol for detection of 
      short, single- or low-copy sequences on plant mitotic metaphase chromosomes.
FAU - Karafiatova, Miroslava
AU  - Karafiatova M
AD  - Centre of the Region Hana for Biotechnological and Agricultural Research, 
      Institute of Experimental Botany, Slechtitelu 31, CZ-78374, Olomouc, Czech 
      Republic. karafiatova@ueb.cas.cz.
FAU - Bartos, Jan
AU  - Bartos J
AD  - Centre of the Region Hana for Biotechnological and Agricultural Research, 
      Institute of Experimental Botany, Slechtitelu 31, CZ-78374, Olomouc, Czech 
      Republic.
FAU - Dolezel, Jaroslav
AU  - Dolezel J
AD  - Centre of the Region Hana for Biotechnological and Agricultural Research, 
      Institute of Experimental Botany, Slechtitelu 31, CZ-78374, Olomouc, Czech 
      Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - *Chromosomes, Plant
MH  - *DNA Copy Number Variations
MH  - DNA, Plant/*genetics
MH  - Hordeum/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mitosis
OTO - NOTNLM
OT  - Cell cycle synchronization
OT  - Cytogenetic mapping
OT  - Fluorochrome
OT  - Low-copy probe
OT  - Mitotic metaphase chromosomes
OT  - Post-fixation
OT  - Probe purification
OT  - Quantum yield
EDAT- 2016/08/12 06:00
MHDA- 2017/10/12 06:00
CRDT- 2016/08/12 06:00
PHST- 2016/08/12 06:00 [entrez]
PHST- 2016/08/12 06:00 [pubmed]
PHST- 2017/10/12 06:00 [medline]
AID - 10.1007/978-1-4939-3622-9_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1429:49-64. doi: 10.1007/978-1-4939-3622-9_5.