PMID- 27512072
OWN - NLM
STAT- MEDLINE
DCOM- 20170509
LR  - 20220208
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Print)
IS  - 0022-538X (Linking)
VI  - 90
IP  - 20
DP  - 2016 Oct 15
TI  - Interrelationship of Primary Virus Replication, Level of Latency, and Time to 
      Reactivation in the Trigeminal Ganglia of Latently Infected Mice.
PG  - 9533-42
LID - 10.1128/JVI.01373-16 [doi]
AB  - We sought to determine the possibility of an interrelationship between primary 
      virus replication in the eye, the level of viral DNA in the trigeminal ganglia 
      (TG) during latency, and the amount of virus reactivation following ocular herpes 
      simplex virus type 1 (HSV-1) infection. Mice were infected with virulent (McKrae) 
      or avirulent (KOS and RE) strains of HSV-1, and virus titers in the eyes and TG 
      during primary infection, level of viral gB DNA in TG on day 28 postinfection 
      (p.i.), and virus reactivation on day 28 p.i. as measured by explant reactivation 
      were calculated. Our results suggest that the avirulent strains of HSV-1, even 
      after corneal scarification, had lower virus titers in the eye, had less latency 
      in the TG, and took a longer time to reactivate than virulent strains of HSV-1. 
      The time to explant reactivation of avirulent strains of HSV-1 was similar to 
      that of the virulent LAT((-)) McKrae-derived mutant. The viral dose with the 
      McKrae strain of HSV-1 affected the level of viral DNA and time to explant 
      reactivation. Overall, our results suggest that there is no absolute correlation 
      between primary virus titer in the eye and TG and the level of viral DNA in 
      latent TG and time to reactivation. IMPORTANCE: Very little is known regarding 
      the interrelationship between primary virus replication in the eye, the level of 
      latency in TG, and the time to reactivate in the mouse model. This study was 
      designed to answer these questions. Our results point to the absence of any 
      correlation between the level of primary virus replication and the level of viral 
      DNA during latency, and neither was an indicator of how rapidly the virus 
      reactivated following explant TG-induced reactivation.
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Matundan, Harry H
AU  - Matundan HH
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research 
      Institute, Los Angeles, California, USA.
FAU - Mott, Kevin R
AU  - Mott KR
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research 
      Institute, Los Angeles, California, USA.
FAU - Allen, Sariah J
AU  - Allen SJ
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research 
      Institute, Los Angeles, California, USA.
FAU - Wang, Shaohui
AU  - Wang S
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research 
      Institute, Los Angeles, California, USA.
FAU - Bresee, Catherine J
AU  - Bresee CJ
AD  - Biostatistics & Bioinformatics Core at the Samuel Oschin Comprehensive Cancer 
      Institute, Cedars-Sinai Burns & Allen Research Institute, Los Angeles, 
      California, USA.
FAU - Ghiasi, Yasamin N
AU  - Ghiasi YN
AD  - Zilkha Neurogenetic Institute, Department of Physiology & Biophysics, and Program 
      in Global Medicine, Keck School of Medicine, University of Southern California, 
      Los Angeles, California, USA.
FAU - Town, Terrence
AU  - Town T
AD  - Zilkha Neurogenetic Institute, Department of Physiology & Biophysics, and Program 
      in Global Medicine, Keck School of Medicine, University of Southern California, 
      Los Angeles, California, USA.
FAU - Wechsler, Steven L
AU  - Wechsler SL
AUID- ORCID: 0000-0001-8509-5229
AD  - The Gavin Herbert Eye Institute and Department of Ophthalmology, University of 
      California, Irvine, School of Medicine, Irvine, California, USA.
FAU - Ghiasi, Homayon
AU  - Ghiasi H
AD  - Center for Neurobiology and Vaccine Development, Ophthalmology Research 
      Laboratory, and Department of Surgery, Cedars-Sinai Burns & Allen Research 
      Institute, Los Angeles, California, USA ghiasih@cshs.org.
LA  - eng
GR  - R21 AI093941/AI/NIAID NIH HHS/United States
GR  - R01 EY024649/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160929
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Animals
MH  - Cornea/virology
MH  - DNA, Viral/genetics
MH  - Disease Models, Animal
MH  - Female
MH  - Herpes Simplex/*virology
MH  - Herpesvirus 1, Human/*genetics
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Trigeminal Ganglion/*virology
MH  - Viral Load/methods
MH  - Virus Activation/*genetics
MH  - Virus Latency/*genetics
MH  - Virus Replication/*genetics
PMC - PMC5044812
EDAT- 2016/08/12 06:00
MHDA- 2017/05/10 06:00
PMCR- 2017/03/29
CRDT- 2016/08/12 06:00
PHST- 2016/07/11 00:00 [received]
PHST- 2016/08/04 00:00 [accepted]
PHST- 2016/08/12 06:00 [entrez]
PHST- 2016/08/12 06:00 [pubmed]
PHST- 2017/05/10 06:00 [medline]
PHST- 2017/03/29 00:00 [pmc-release]
AID - JVI.01373-16 [pii]
AID - 01373-16 [pii]
AID - 10.1128/JVI.01373-16 [doi]
PST - epublish
SO  - J Virol. 2016 Sep 29;90(20):9533-42. doi: 10.1128/JVI.01373-16. Print 2016 Oct 
      15.