PMID- 27515534
OWN - NLM
STAT- MEDLINE
DCOM- 20171117
LR  - 20190122
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 7
IP  - 1
DP  - 2016 Aug 11
TI  - Human umbilical cord-derived mesenchymal stem cells protect against experimental 
      colitis via CD5(+) B regulatory cells.
PG  - 109
LID - 10.1186/s13287-016-0376-2 [doi]
LID - 109
AB  - BACKGROUND: To clarify the effect of human umbilical cord-derived mesenchymal 
      stem cell (hUC-MSCs) treatment on colitis and to explore the role of CD5(+) B 
      cells in MSC therapy. METHODS: The trinitrobenzenesulfonic acid (TNBS)-induced 
      colitis mouse model was used. HUC-MSCs were transferred peritoneally. Survival 
      rates, colitis symptoms, and macroscopic and histologic scores were evaluated. 
      CD4(+) T helper (Th) cell subgroups and CD5(+) regulatory B cell (Bregs) in 
      lymphocytes were quantitated by flow cytometry. Cytokine levels were detected by 
      ELISA and Bio-plex. CD5(+) B cells were isolated for in vitro co-culture and 
      adaptive transfer. RESULTS: HUC-MSC treatment alleviated TNBS-induced colitis by 
      increasing survival rates, relieving symptoms, and improving macroscopic and 
      histologic scores. Labeled hUC-MSCs were located in the inflamed areas of colitis 
      mice. Increases in regulatory T cells (Tregs) and CD5(+) B cells and decreases in 
      Th1 cells, Th17 cells, and several pro-inflammatory cytokines were observed with 
      hUC-MSC treatment. After adaptive transfer, CD5(+) B cells, which were located 
      mainly in the peritoneal lavage fluid, improved TNBS-induced colitis by 
      correcting Treg/Th1/Th17 imbalances. CD5(+) B cells also inhibited T-cell 
      proliferation and produced interleukin (IL)-10. CONCLUSIONS: HUC-MSCs protected 
      against experimental colitis by boosting the numbers of CD5(+) B cells and 
      IL-10-producing CD5(+) Bregs, and correcting Treg/Th17/Th1 imbalances.
FAU - Chao, Kang
AU  - Chao K
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
AD  - Division of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510655, People's Republic of China.
FAU - Zhang, Shenghong
AU  - Zhang S
AUID- ORCID: 0000-0002-1647-0563
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China. 
      shenghongzhang@163.com.
FAU - Qiu, Yun
AU  - Qiu Y
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
FAU - Chen, Xiaoyong
AU  - Chen X
AD  - Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem 
      Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, 
      Guangzhou, 510080, People's Republic of China.
FAU - Zhang, Xiaoran
AU  - Zhang X
AD  - Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem 
      Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, 
      Guangzhou, 510080, People's Republic of China.
FAU - Cai, Chuang
AU  - Cai C
AD  - Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem 
      Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, 
      Guangzhou, 510080, People's Republic of China.
FAU - Peng, Yanwen
AU  - Peng Y
AD  - Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem 
      Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, 
      Guangzhou, 510080, People's Republic of China.
FAU - Mao, Ren
AU  - Mao R
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
FAU - Pevsner-Fischer, Meirav
AU  - Pevsner-Fischer M
AD  - Department of Immunology, Weizmann Institute of Science, Rehovot, 7610001, 
      Israel.
FAU - Ben-Horin, Shomron
AU  - Ben-Horin S
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
FAU - Elinav, Eran
AU  - Elinav E
AD  - Department of Immunology, Weizmann Institute of Science, Rehovot, 7610001, 
      Israel.
FAU - Zeng, Zhirong
AU  - Zeng Z
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
FAU - Chen, Baili
AU  - Chen B
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
FAU - He, Yao
AU  - He Y
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China.
FAU - Xiang, Andy Peng
AU  - Xiang AP
AD  - Center for Stem Cell Biology and Tissue Engineering, The Key Laboratory for Stem 
      Cells and Tissue Engineering, Ministry of Education, Sun Yat-Sen University, 
      Guangzhou, 510080, People's Republic of China. xiangp@mail.sysu.edu.cn.
FAU - Chen, Minhu
AU  - Chen M
AD  - Division of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, People's Republic of China. chenminhu@vip.163.com.
LA  - eng
PT  - Journal Article
DEP - 20160811
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (CD5 Antigens)
RN  - 130068-27-8 (Interleukin-10)
RN  - 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid)
SB  - IM
EIN - Stem Cell Res Ther. 2019 Jan 21;10(1):33. PMID: 30665468
MH  - Animals
MH  - CD5 Antigens/*immunology
MH  - Cell Proliferation/physiology
MH  - Colitis/chemically induced/*immunology/*therapy
MH  - Disease Models, Animal
MH  - Humans
MH  - Inflammation/immunology
MH  - Interleukin-10/immunology
MH  - Male
MH  - Mesenchymal Stem Cells/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Stem Cells/*immunology
MH  - Survival Rate
MH  - T-Lymphocytes, Regulatory/*immunology
MH  - Th1 Cells/immunology
MH  - Th17 Cells/immunology
MH  - Trinitrobenzenesulfonic Acid/pharmacology
MH  - Umbilical Cord/*immunology
PMC - PMC4981968
OTO - NOTNLM
OT  - B regulatory cell
OT  - Colitis
OT  - Crohn's disease
OT  - Mesenchymal stem cells
OT  - T helper cell
EDAT- 2016/08/16 06:00
MHDA- 2017/11/29 06:00
PMCR- 2016/08/11
CRDT- 2016/08/13 06:00
PHST- 2016/05/07 00:00 [received]
PHST- 2016/07/26 00:00 [accepted]
PHST- 2016/07/13 00:00 [revised]
PHST- 2016/08/13 06:00 [entrez]
PHST- 2016/08/16 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2016/08/11 00:00 [pmc-release]
AID - 10.1186/s13287-016-0376-2 [pii]
AID - 376 [pii]
AID - 10.1186/s13287-016-0376-2 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2016 Aug 11;7(1):109. doi: 10.1186/s13287-016-0376-2.